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Immobilized lipase particle

A technology for immobilizing lipase and lipase, applied in the direction of enzyme, immobilized on or in inorganic carrier, hydrolase, etc., can solve the problems of fragility, mass transfer limitation, poor stability of enzyme activity, etc., and achieve high enzymatic activity. Activity, mass transfer limitation reduction, effect of improving catalytic efficiency

Active Publication Date: 2017-07-07
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such products have problems such as poor stability of enzyme activity, limited mass transfer, and easy breakage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] With 100ml TL (Thermomyces lanuginose) lipase liquid (Novozyme Company, trade name lipozyme TL100L), 20g maltodextrin (Henan Yuzhong Biotechnology Co., Ltd.), 1.5g sodium carboxymethylcellulose (Sinopharm Reagent), 80ml water, mix well, add 10 g soybean oil (Kerry Foods), shear in a homogenizer (Fluka) for 2 min, set aside. Another 100g of white carbon black (model: 5162Q, Qiubo Engineering Materials Co., Ltd.; average particle size 50 μm) was put into a high-speed mixing granulator (Changzhou Jiafa Drying Equipment Company; model GHL-7.5), and the prepared enzyme The liquid is sprayed into the granulator. When the particle becomes larger to 200-600 microns, take out the wet material and extrude it in a laboratory extrusion granulator whose model is (JZL-80 Changzhou Yongchang Granulating and Drying Equipment Co., Ltd.). The extruded soft material is sent to a shot blasting machine (Changzhou Jiafa Drying Equipment Co., Ltd.; model QZL MINI) for granulation, and the th...

Embodiment 2

[0063] Embodiment 2 - comparative example

[0064] Mix 100ml TL (Thermomyces lanuginose) lipase solution (Novozymes, trade name lipozyme TL100L), 20g maltodextrin (Henan Yuzhong Biotechnology Co., Ltd.), 1.5g sodium carboxymethylcellulose, and 80ml water spare. Another 100g zeofree white carbon black (model: 5162Q, Huber Engineering Materials Co., Ltd.; average particle size 50 μm) was put into a high-speed mixing granulator (Changzhou Jiafa Drying Equipment Company; model GHL-10), and the prepared The enzyme solution was sprayed into a granulator (model QZL MINI; Changzhou Jiafa Drying Equipment Co., Ltd.). When the particles become larger to 200-600 microns, the wet material is taken out and extruded in an extruder. The extruded soft material is sent to the shot blasting machine for granulation, and the thrown sample is dried under ventilated natural conditions until the moisture content is below 8% by weight.

Embodiment 3

[0066] Mix 100ml TL (Thermomyces lanuginose) lipase solution, 20g maltodextrin (Henan Yuzhong Biotechnology Co., Ltd.), 1.5g sodium carboxymethylcellulose, and 80ml water and set aside. Another 120g Aerosil 200 white carbon black (Degussa Company; average particle size 30 μm) and 15g palm stearin powder (Kerry Special Oil Shanghai Co., Ltd.) were put into a high-speed mixing granulator (Changzhou Jiafa Drying Equipment Company; model GHL-10), spray the prepared enzyme liquid into the granulator. When the particle becomes larger to 200-600 microns, take out the wet material and extrude it in the extruder. The extruded soft material is sent to the shot blasting machine for granulation, and the thrown sample is dried under ventilated natural conditions until the moisture content is below 8% by weight.

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Abstract

The invention relates to a stable immobilized lipase product and a preparation method thereof. By the immobilized lipase product with high random ester exchange enzyme activity, mass transfer limitation is reduced.

Description

technical field [0001] The invention relates to the field of enzyme products. Specifically, the invention relates to an immobilized lipase particle and a preparation method thereof. Background technique [0002] The full name of lipase (lipase, EC 3.1.1.3) is triacylglycerol hydrolase. Lipases from different microorganisms also have functions such as esterification, transesterification, alcoholysis, and racemate resolution, and are therefore widely used in various fields including food, medicine, and daily chemicals. Because it is difficult to separate and cannot be reused, liquid lipase has limited industrial application. For this reason, the enzyme has been shaped on a carrier with certain physical properties through immobilization technology, so as to facilitate separation and reuse. [0003] At present, enzyme immobilization technology is mainly divided into two categories: inorganic or organic polymer powder materials, and organic polymer resin. Among them, the poly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N9/20C12R1/645C12R1/785C12R1/38C12R1/845C12R1/685C12R1/01
CPCC12N9/20C12N11/14C12Y301/01003
Inventor 辛本荣郑妍杨武林李效刚
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
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