Immobilized lipase particle
A technology for immobilizing lipase and lipase, applied in the direction of enzyme, immobilized on or in inorganic carrier, hydrolase, etc., can solve the problems of fragility, mass transfer limitation, poor stability of enzyme activity, etc., and achieve high enzymatic activity. Activity, mass transfer limitation reduction, effect of improving catalytic efficiency
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Embodiment 1
[0062] With 100ml TL (Thermomyces lanuginose) lipase liquid (Novozyme Company, trade name lipozyme TL100L), 20g maltodextrin (Henan Yuzhong Biotechnology Co., Ltd.), 1.5g sodium carboxymethylcellulose (Sinopharm Reagent), 80ml water, mix well, add 10 g soybean oil (Kerry Foods), shear in a homogenizer (Fluka) for 2 min, set aside. Another 100g of white carbon black (model: 5162Q, Qiubo Engineering Materials Co., Ltd.; average particle size 50 μm) was put into a high-speed mixing granulator (Changzhou Jiafa Drying Equipment Company; model GHL-7.5), and the prepared enzyme The liquid is sprayed into the granulator. When the particle becomes larger to 200-600 microns, take out the wet material and extrude it in a laboratory extrusion granulator whose model is (JZL-80 Changzhou Yongchang Granulating and Drying Equipment Co., Ltd.). The extruded soft material is sent to a shot blasting machine (Changzhou Jiafa Drying Equipment Co., Ltd.; model QZL MINI) for granulation, and the th...
Embodiment 2
[0063] Embodiment 2 - comparative example
[0064] Mix 100ml TL (Thermomyces lanuginose) lipase solution (Novozymes, trade name lipozyme TL100L), 20g maltodextrin (Henan Yuzhong Biotechnology Co., Ltd.), 1.5g sodium carboxymethylcellulose, and 80ml water spare. Another 100g zeofree white carbon black (model: 5162Q, Huber Engineering Materials Co., Ltd.; average particle size 50 μm) was put into a high-speed mixing granulator (Changzhou Jiafa Drying Equipment Company; model GHL-10), and the prepared The enzyme solution was sprayed into a granulator (model QZL MINI; Changzhou Jiafa Drying Equipment Co., Ltd.). When the particles become larger to 200-600 microns, the wet material is taken out and extruded in an extruder. The extruded soft material is sent to the shot blasting machine for granulation, and the thrown sample is dried under ventilated natural conditions until the moisture content is below 8% by weight.
Embodiment 3
[0066] Mix 100ml TL (Thermomyces lanuginose) lipase solution, 20g maltodextrin (Henan Yuzhong Biotechnology Co., Ltd.), 1.5g sodium carboxymethylcellulose, and 80ml water and set aside. Another 120g Aerosil 200 white carbon black (Degussa Company; average particle size 30 μm) and 15g palm stearin powder (Kerry Special Oil Shanghai Co., Ltd.) were put into a high-speed mixing granulator (Changzhou Jiafa Drying Equipment Company; model GHL-10), spray the prepared enzyme liquid into the granulator. When the particle becomes larger to 200-600 microns, take out the wet material and extrude it in the extruder. The extruded soft material is sent to the shot blasting machine for granulation, and the thrown sample is dried under ventilated natural conditions until the moisture content is below 8% by weight.
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