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Preparation method for agent capable of promoting growth gene expression of pigs

A technology of gene expression and methyl group, applied in the field of synthesis of amino acid derivatives, can solve problems such as long synthesis route, and achieve the effects of stable quality, simple preparation method and growth promotion.

Inactive Publication Date: 2017-07-04
CHANGSHA POWERLIFE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since these methods are only suitable for the N-methylation of some amino acids, in addition, the synthetic routes in these methods are relatively long, and there is also racemization of the substrate during the methylation process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method of a pig growth gene expression promoter, comprising the steps of:

[0019] (1) Preparation of D-aspartic acid dimethyl ester hydrochloride: add 53.3 grams (0.4mol) of D-aspartic acid and 300ml of methanol in the reaction vessel, slowly add 44.2ml (0.6mol) ) of thionyl chloride, control the reaction temperature at 0°C, after the addition of thionyl chloride, continue stirring at room temperature at 28°C, after 18 hours of reaction, the reaction solution is decompressed to remove excess methanol and thionyl chloride, The remaining oil was crystallized with 2 times of diethyl ether, and the obtained white crystals were suction-filtered, washed three times with diethyl ether, and dried to obtain 45.2 grams of D-aspartic acid dimethyl hydrochloride as a white solid, melting point 114-116°C ;

[0020] (2) Preparation of N-hydroxymethyl-D-aspartic acid dimethyl ester: add 31.2 grams (0.2mol) of D-aspartic acid dimethyl ester hydrochloride and 50ml of 40%...

Embodiment 2

[0025] A preparation method of a pig growth gene expression promoter, comprising the steps of:

[0026] (1) Preparation of D-aspartic acid dimethyl ester hydrochloride: add 79.8 grams (0.6mol) of D-aspartic acid and 400ml of methanol in the reaction vessel, slowly add 56.2ml (0.8mol) ) of thionyl chloride, control the reaction temperature at 0°C, after the addition of thionyl chloride, continue to stir at room temperature at 30°C, and react for 20 hours, distill off excess methanol and thionyl chloride from the reaction solution under reduced pressure, The remaining oil was crystallized with 2-4 times of diethyl ether, and the obtained white crystals were suction filtered, washed three times with diethyl ether, and dried to obtain 69.6 grams of D-aspartic acid dimethyl hydrochloride as a white solid, melting point 143- 145°C;

[0027] (2) Preparation of N-hydroxymethyl-D-aspartic acid dimethyl ester: add 62.4 grams (0.4mol) of D-aspartic acid dimethyl ester hydrochloride and ...

Embodiment 3

[0032] A preparation method of a pig growth gene expression promoter, comprising the steps of:

[0033] (1) Preparation of D-aspartic acid dimethyl ester hydrochloride: add 106.8 grams (0.8mol) of D-aspartic acid and 700ml of methanol in the reaction vessel, slowly add 84.4ml (1.2mol) of methanol under stirring ) of thionyl chloride, control the reaction temperature at 0°C, after the addition of thionyl chloride, continue stirring at room temperature at 29°C, after 30 hours of reaction, the reaction solution is decompressed to remove excess methanol and thionyl chloride, The remaining oil was crystallized with 3.5 times of diethyl ether, and the obtained white crystals were suction filtered, washed three times with diethyl ether, and dried to obtain 107.8 grams of D-aspartic acid dimethyl ester hydrochloride as a white solid;

[0034] (2) Preparation of N-hydroxymethyl-D-aspartic acid dimethyl ester: add 78 grams (0.5mol) of D-aspartic acid dimethyl ester hydrochloride and 120...

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Abstract

The invention discloses a preparation method for an agent capable of promoting growth gene expression of pigs. The invention relates to synthesis of amino acid derivatives, specifically to a preparation method for a growth-gene-expression promoting agent N-methyl-D-aspartic acid, i.e., a derivative of D-aspartic acid. The preparation method comprises the following steps: with D-aspartic acid as a raw material, reacting D-aspartic acid with methanol and thionyl chloride so as to produce dimethyl-D-aspartate hydrochloride; then subjecting dimethyl-D-aspartate hydrochloride and 40% formaldehyde to hydroxymethylation under an alkaline condition so as to produce N-hydroxymethyl-D-aspartic acid; and then carrying out hydrogenation at normal temperature and normal pressure under the action of a Pd / C catalyst so as to obtain N-methyl-D-aspartic acid. The preparation method is simple; the prepared N-methyl-D-aspartic acid is stable in quality; no environmental pollution is produced during preparation; and the prepared N-methyl-D-aspartic acid can promote the growth of livestock and poultry, improves the utilization rate of feeds, the carcass quality of pigs and the rate of lean meat, and is convenient for promotion and application.

Description

technical field [0001] The invention relates to the synthesis of an amino acid derivative, in particular to a method for preparing a derivative of pig growth gene expression promoter D-aspartic acid, N-methyl-D-aspartic acid. Background technique [0002] N-methyl-D-aspartic acid is the methylation product of D-aspartic acid. Generally only exists in the neuroendocrine tissues of humans and animals. Many studies have shown that an appropriate amount of N-methyl-D-aspartic acid can promote the secretion of growth hormone in the pituitary gland of animals, and is a new type of feed additive with high efficiency and growth promotion. In recent years, with the continuous development of molecular biology and cell biology, the in-depth research on its mechanism of action has confirmed that N-methyl-D-aspartic acid is a strong inhibitor of neuroexcitatory amino acid transmitter aspartic acid. It is an effective activator, participates in neuroendocrine function, and effectively i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C227/18C07C229/24
CPCC07C227/16C07C227/18C07C229/24
Inventor 刘继根
Owner CHANGSHA POWERLIFE BIOTECH
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