Primers for detecting polymorphism of FTO rs79206939 gene and PCR (polymerase chain reaction) method

Abstract

The invention discloses a primer pair FTOF / FTOR for detecting a polymorphic site of a FTO rs16957920 gene by a PCR (polymerase chain reaction) method. Sequences are respectively shown in SEQ ID NO.1 and NO.2. The invention also discloses application of the primer pair FTOF / FTOR in detecting the polymorphic site of the FTO rs16957920 gene of a biological sample, and a kit prepared by the primer pair for detecting the polymorphic site of the FTO rs16957920 gene. The primer pair and the PCR method have the advantages that the gene type of the polymorphic site of the FTO rs16957920 gene can be determined according to the amplification and electrophoresis results, the therapy effect and side effect of a purinethol drug can be predicted, the validity and safety of a patient using the purinethol drug can be predicted on the basis of gene type analysis, the clinical drug application is instructed, and the individualized treatment of the patient can be performed.

Description

technical field [0001] The invention belongs to the field of medical biotechnology. More specifically, it relates to a primer for detecting FTO rs79206939 gene polymorphism and a PCR method thereof. Background technique [0002] Mercaptopurines are represented by 6-mercaptopurine, 6-thioguanine and their precursor form azathioprine, which are effective against hematological tumors, inflammatory bowel disease, villi The treatment of membranous epithelial carcinoma and malignant mole has a good curative effect. In addition, it is also widely used in the treatment of many common clinical diseases such as surgery, gastroenterology and dermatology. [0003] However, the metabolism of these drugs in the body is complex, there are individual differences in efficacy and toxicity, and the incidence of adverse reactions is high, including bone marrow suppression, liver damage, gastrointestinal disorders, pancreatitis, and influenza-like symptoms. Studies have found that although man...

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Problems solved by technology

[0003] However, the metabolism of these drugs in the body is complex, there are individual differences in efficacy and toxicity, and the incidence of adverse reactions is high, including bone marrow suppression, liver damage, gastrointestinal disorders, pancreatitis, and influenza-like symptoms, etc.

Studies have found that although many factors, such as gender, age, liver and kidney function, etc. may affect the final results of drug treatment, genes are still the main reason for individual differences in drug use

[0004] At present, the research on gene polymorphisms of thiopurine drug metabolizing enzymes and transporters is relatively in-depth. For example, TPMT gene polymorphisms may predict the occurrence of adverse reactions

However, the mutation rate is low, the sensitivity is low, and the clinical application prospect is not very clear

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