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Method for extracting thuja essential oil by fermentation with microbial system

A technology for thuja essential oil and microorganisms is applied in the extraction of thuja essential oil, and the field of thuja essential oil is extracted by fermentation with a microbial system, so as to achieve the effects of simple process, improved extraction rate and increased yield

Active Publication Date: 2019-08-06
广州美崖子生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings and deficiencies of various essential oil extraction methods in the prior art, the object of the present invention is to provide a method for extracting thuja essential oil by fermentation with a microbial system

Method used

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  • Method for extracting thuja essential oil by fermentation with microbial system
  • Method for extracting thuja essential oil by fermentation with microbial system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Get 10kg of thuja raw material, crush it into sawdust; adjust the moisture content to 40%, add 2% sucrose, 0.5% yeast extract, 0.5% Trichoderma viride spore powder (200 million / g), mix well and place in a solid reactor Fermentation at 25-30°C with intermittent aeration and stirring for 7-15 days, the aeration rate is 0.07-0.1vvm, 1-3 minutes each time, to promote the disintegration of thuja raw material cellulose or lignin structure; then fermented The Thuja raw material was placed in a steam distillation extractor for distillation and extraction for 7 hours, the oil yield was 1.13kg, and the essential oil yield was 11.3%.

Embodiment 2

[0021] Get 10kg of thuja raw material, crush it into sawdust; adjust the moisture content to 50%, add 2% sucrose, 0.5% yeast extract, 5% Trichoderma viride spore powder (2 billion / g), mix well and place in a solid reactor Fermentation at 25-30°C with intermittent aeration and stirring for 7-15 days, the aeration rate is 0.07-0.1vvm, 1-3 minutes each time, to promote the disintegration of thuja raw material cellulose or lignin structure; then fermented The Thuja raw material was placed in a steam distillation extractor for distillation and extraction for 7 hours, the oil yield was 1.511kg, and the essential oil yield was 15.1%.

Embodiment 3

[0023] Get 10kg of thuja raw material, crush it into sawdust; adjust the moisture content to 45%, add 2% sucrose, 0.5% yeast extract, 2.5% Trichoderma korningii spore powder (200 million / g) and 2.5% Trichoderma viride spore powder (1,000,000,000 / g), mixed evenly, placed in a solid reactor, and fermented at 25-30°C with intermittent aeration for 7-15 days, with an aeration rate of 0.07-0.1vvm, 1-3 minutes each time, to promote The cellulose or lignin structure of the cypress raw material was disintegrated; then the fermented thuja raw material was distilled and extracted for 7 hours in a steam distillation extractor, and the oil yield was 1.31kg, and the yield of essential oil was 13.1%.

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Abstract

The invention discloses a method for extracting platycladus orientalis (L.) franco essential oil through microorganism system fermentation and belongs to the field of biological extraction. The method comprises the steps of smashing a platycladus orientalis (L.) franco raw material into wood bits, adding water to adjust moisture content of the wood bits, adding mycotic spore powder, then adding cane sugar and yeast extractive, evenly mixing, aerating at intervals and stirring to ferment; finally performing steam distillation on the fermented platycladus orientalis (L.) franco raw material to extract essential oil and collecting oil to obtain platycladus orientalis (L.) franco essential oil. The method disclosed by the invention has a simple technology, utilizes characteristics that specific mould resolves fiber and a wooden structure to control a decomposition effect of effective microorganism and accordingly effectively improves the extracting rate of the platycladus orientalis (L.) franco essential oil; the yield of the platycladus orientalis (L.) franco essential oil is 10% to 15% and is far higher than yield as 1% to 4% of a general extracting method; the content of a main functional ingredient alpha-cedrol in the essential oil is about 100% higher than that of the general steam distillation method; the method has strong operability, is suitable for large-scale production, improves the yield of the platycladus orientalis (L.) franco essential oil and is suitable for extracting essential oil from varieties of sources and portions of the latycladus orientalis (L.) franco raw material.

Description

technical field [0001] The invention belongs to the field of biological extraction, and relates to a method for extracting thuja essential oil, in particular to a method for extracting thuja essential oil by fermentation with a microbial system. Background technique [0002] Platycladus orientalis (L.) Franco belongs to the green tree, Cupressaceae plant, also known as cypress, cypress, etc., is a special product of our country. Arborvitae has a long lifespan, and there are often ancient trees of hundreds of years or more. "Compendium of Materia Medica" defines cypress as "top-grade fragrant wood", and "Historical Records" even defines cypress as "the best of all trees". The arborvitae that grow on rocks or cliffs are commonly known as thuja. Because Thuja mostly grows in areas with poor nutrition and harsh environment, its root system winds among the rocks, so its wood density is high and oily. Thuja is mainly distributed in the Taihang Mountains, Qinling Mountains, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/00C11B9/02C12R1/885
CPCC11B9/027C12P33/00
Inventor 俞巍蔚林彩娟司卫丽任杰申彦晶
Owner 广州美崖子生物科技有限公司
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