Micro-plant hydrotrope allelopathic potential evaluation bioassay method

A bioassay, plant technology, applied in the fields of botanical equipment and methods, horticultural methods, horticulture, etc., can solve problems such as the influence of bioassay results, the influence of the growth of recipient plants, and the vulnerability of plant water extracts to microorganisms. Ease of handling effects

Inactive Publication Date: 2017-05-10
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for rare plants, small quantities, and limited access to materials, the water extract method is not feasible, and if a large number of plant extracts are aseptically processed and stored, the cost of the test will be increased. In addition, due to different concentrations of Differences in osmotic potential of water extracts affect bioassay results 10 Therefore, the existing plant water extract bioassay method has the following defects and limitations: (1) it is not easy to obtain the evaluation of the allelopathic potential of biological materials, (2) the technology that the plant water extract is easily affected by microorganisms in the environment Question, (3) Effect of different concentration osmotic potential of water extract on the growth of recipient plants

Method used

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  • Micro-plant hydrotrope allelopathic potential evaluation bioassay method
  • Micro-plant hydrotrope allelopathic potential evaluation bioassay method
  • Micro-plant hydrotrope allelopathic potential evaluation bioassay method

Examples

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Effect test

Embodiment 1

[0040] The test materials are rice and barnyardgrass, among which rice is the donor plant and the following five varieties are used: the reported non-allelopathic variety Lemont, the reported allelopathic variety PI312777, Koukesumochi, Dongjingbyeo and K21; the recipient plant is barnyardgrass . The test was repeated 3 times.

[0041] The steps of this embodiment are as follows:

[0042] 1. Culture medium preparation: Prepare agar medium with a concentration of 6% in terms of mass fraction with water, sterilize at 120°C and 0.1MPa for 20 minutes, and then pour them into round and square petri dishes for use;

[0043] 2. Donor plants are the 5 kinds of rice mentioned above. After the rice seeds are fully absorbed, put them on a petri dish and place them in an incubator at 28°C for 48 hours to accelerate germination, then sow them in small pots filled with soil, and place them Plant in a greenhouse under 28°C and 12 hours of light / dark conditions for 20 days, cut the abovegro...

Embodiment 2

[0051] The test materials were rice and lettuce. Rice was the donor plant and the following three varieties were used: the reported non-allelopathic variety Lemont, the reported allelopathic variety PI312777 and Koukesumochi; the recipient plant was lettuce. The test was repeated 3 times.

[0052] The steps of this embodiment are as follows:

[0053] 1. Preparation of culture medium: prepare agar medium with a concentration of 6% in terms of mass fraction with water, pour it into round and square petri dishes for use after ordinary high-pressure sterilization;

[0054] 2. Donor plants are the three kinds of rice mentioned above. Put the rice seeds on a petri dish after fully absorbing water and place them in an incubator at 25°C for 48 hours to germinate, then sow them in small pots filled with soil, and place them Plant in a greenhouse under 25°C and 12-hour light / dark conditions for 30 days, cut the above-ground parts of rice, and sterilize the above-ground parts of differe...

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Abstract

The invention discloses a micro-plant hydrotrope allelopathic potential evaluation bioassay method. The method comprises the steps of using an overground part of donor plants with 20-30 days, cutting the overground part into 1-2 cm after surface sterilization, leaching the cut parts in sterile water at 4 DEG C for 24 h, conducting film filtering and sterilization, and obtaining plant water leaching liquor; conducting the same sterilization on receptor plant seeds and then sowing the receptor plant seeds into a petri dish which contains 6% of agal-agal, when roots are downwards perpendicularly cultivated till the length of the root is 0.5-1 cm, adding 5-10 microliter of the donor plant water leaching liquor, continuing cultivation for 2-3 days in a culture room at 25 or 28 DEG C for 12 hours light / dark culture and then taking out the donor plant water leaching liquor to measure amount of growth, comparing the amount of growth of the donor plants with that of the receptor plants which are not affected by the donor plants, and calculating influence conditions of plant root system secretions on the growth of the receptor plants to conduct allelopathic potential evaluation. The micro-plant hydrotrope allelopathic potential evaluation bioassay method has the advantages that only few donor plants are needed to extract water-soluble matter, there is no need to adjust osmotic pressure, bioactivity of plant water-soluble matter can be reflected while microbial interference can be controlled to the greatest extent, and the micro-plant hydrotrope allelopathic potential evaluation bioassay method is a simple, fast, economical and accurate bioassay method.

Description

technical field [0001] The invention relates to a bioassay method for evaluating the allelopathic potential of trace plant water-soluble substances, belonging to the technical field of bioassays. Background technique [0002] The phenomenon of mutual growth and mutual restraint among organisms has been recognized by humans since ancient times, and it is used in agricultural production to arrange crop rotation and stubble. In 1996, the International Allelopathy Society (International Allelopathy Society) defined allelopathy as: an individual organism affects the growth and development of other organisms around it by releasing compounds into the environment. There are organic and inorganic substances released by plants to the environment, and their forms include: root exudation, volatile gases, leaching substances, etc. Plant allelopathy is a chemical expression of plant adaptation to the environment. Plant roots, stems, leaves and other organs or tissues release "allelochemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06
CPCA01G7/06
Inventor 郭怡卿孙一丁付坚陆永良刘亚洲汤东生王玲仙
Owner YUNNAN AGRICULTURAL UNIVERSITY
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