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Method for inhibiting and/or damaging biological membrane

A biofilm and compound technology, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve problems such as insensitivity to antibiotics, slow growth of bacteria, and inability to eradicate biofilms, so as to avoid Drug resistance, the effect of suppressing the development of drug resistance

Active Publication Date: 2017-06-20
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if antibacterial drugs penetrate into the biofilm, the bacteria inside grow slowly or are in a dormant period due to factors such as lack of nutrients and oxygen, and are extremely insensitive to antibiotics, which is the main reason why biofilms cannot be eradicated
Finding new therapeutic strategies to inhibit biofilm formation while disrupting mature biofilms due to drug resistance is challenging

Method used

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  • Method for inhibiting and/or damaging biological membrane
  • Method for inhibiting and/or damaging biological membrane
  • Method for inhibiting and/or damaging biological membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0045] According to a specific embodiment of the present invention, the step of detecting the effect of destroying biofilm may be: after contacting the compound represented by formula (I) with a sample containing biofilm (such as bacterial liquid) and illuminating, ultrasonic vibration Disperse the biofilm; dilute the dispersed bacteria to a certain number of times, spread 100 μL of the diluted solution on the solid medium, and count the number of colonies on the plate after culturing at 37°C for 20 hours; then calculate the inhibition rate of the biofilm according to formula (III) IR. The dilution factor can be determined according to the number of bacteria, so that the number of colonies on the solid medium is convenient for statistics.

[0046] In the present invention, the amount of the sample containing biofilm contacted with the compound represented by formula (I) can vary within a wide range. When the sample containing biofilm is a liquid sample containing bacteria, the...

Embodiment 1

[0063] This embodiment is used to illustrate the synthesis of compound PFP shown in formula (I)

[0064] The specific structure of PFP is:

[0065]

[0066] 9,9-bis(6′-bromohexyl)-2,7-dibromofluorene: 1,6-dibromohexane (97.6g, 400mmol) was added to 100ml of 50% potassium hydroxide solution, and 1.28g was added for phase transfer Catalyst tetrabutylammonium bromide (TBAB), the temperature of the reaction solution was raised to 75°C, and then 2,7-dibromofluorene (12.96g, 40mmol) was added and stirred for 15min. After the reaction was stopped and cooled to room temperature, extracted with dichloromethane (100ml×3), the combined organic phases were washed with 1M hydrochloric acid solution and distilled water, dried over anhydrous magnesium sulfate, filtered, and concentrated. The crude product was separated and purified by silica gel column chromatography, and the developing solvent was dichloromethane / petroleum ether (v / v=1 / 9) to obtain the white solid product 9,9-bis(6′-bro...

Embodiment 2

[0072] This example is used to illustrate the revived culture of Staphylococcus aureus

[0073] Using Staphylococcus aureus ATCC 6538 as a template, use 75% alcohol absorbent cotton to sterilize the outer surface of the purchased ampoule containing Staphylococcus aureus ATCC 6538 in the ultra-clean workbench, heat the top of it with a flame, and drop 400 μL without Bacterial water to the top of the heated ampoule to rupture it. Draw 300 μL of suitable liquid culture medium (can be replaced by sterile water), drop it into the ampoule bottle, shake and blow gently, so that the freeze-dried bacteria dissolve into a suspension, absorb all the bacterial suspension, and transplant them into two NB solid culture medium respectively. Incubate at 37°C for 20 hours. Use an inoculation loop to scrape an appropriate amount of bacterium and inoculate it into a new NB solid medium, and continue to cultivate at 37°C. Continue to culture for 3-4 generations in this way to obtain a stable str...

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Abstract

The invention relates to the field of biological membranes, and discloses a method for inhibiting and / or damaging a biological membrane. The method comprises the step of contacting a compound as shown in a formula (I) and a sample, wherein the sample is a sample containing the biological membrane and / or a sample capable of forming but not forming the biological membrane. By adopting the method provided by the invention, the formation of the biological membrane can be inhibited, and the mature biological membrane can be damaged. The formula (I) is shown in the description, wherein R1#, R2#, R3#, R4#, R1*, R2*, R3* and R4* are independently alkyl groups of C1 to C12; X# and X* independently express halogen; n is an integer ranging from 8 to 15; the number-average molecular weight of the compound as shown in the formula (I) ranges from 5000 to 10000.

Description

technical field [0001] The invention relates to the field of biofilms, in particular to a method for inhibiting and / or destroying biofilms. Background technique [0002] Biofilm refers to a large number of bacterial aggregation films formed by microorganisms in order to adapt to the environment, adhere to the surface of non-biological or active tissues, secrete a large amount of heterogeneous extracellular matrix such as polysaccharides, proteins and nucleic acids, and wrap the bacteria themselves in it. The sample is a common living state of bacteria in nature. The formation of biofilm is divided into the following stages: 1. Initial adhesion, free bacteria adhere to the surface of solid substrate and secrete extracellular nucleic acid to promote adhesion. 2. The formation of micro-colonies, the adhered bacteria form micro-colonies after reproduction and amplification, and secrete extracellular proteins to promote adhesion. 3. When the biofilm matures, the bacteria grow f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/00A01P1/00A01P3/00
CPCA01N25/00
Inventor 王树张鹏博刘礼兵吕凤婷
Owner INST OF CHEM CHINESE ACAD OF SCI
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