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Preparation method and application of bone marrow protein

A protein and bone marrow technology, applied in the field of bone marrow protein preparation, can solve problems such as environmental pollution, environmental pressure, waste of resources, etc.

Active Publication Date: 2017-06-16
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] my country is a big consumer of livestock and poultry, but most of them focus on the consumption of livestock and poultry meat. A large number of livestock and poultry bones are not fully utilized. pollute the environment and cause certain environmental pressure

Method used

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  • Preparation method and application of bone marrow protein
  • Preparation method and application of bone marrow protein
  • Preparation method and application of bone marrow protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Buffer extraction:

[0036] a. Take fresh bone marrow, wash it 3 times, remove the bones and blood, cut it into small pieces, add liquid nitrogen at a weight ratio of 1:5, and grind it repeatedly for 3 times to obtain bone marrow powder, and store it in a low-temperature refrigerator for later use;

[0037] b. Take 100 g of the crushed bone marrow powder obtained in step a, add 0.1 mol / L tris hydrochloride with a weight ratio of 1:10, pH=8.4 buffer solution, and set intensive constant temperature heating at a temperature of 40°C Reflux on a magnetic stirrer and extract repeatedly 2 times until no precipitate is seen to obtain the total extract;

[0038] C, the total extraction that step b obtains is degreased by the sherwood oil of 1:6 according to the solid-liquid ratio, repeats 2-3 times, collects the lower layer extract;

[0039] d. Centrifuge the lower layer extract obtained in step c at 12,000 rpm at 4° C. for 10 minutes, and take the supernatant to obtain a crude...

Embodiment 2

[0043] Inorganic salt solvent extraction:

[0044] a. Take fresh bone marrow, wash it 3 times, remove the bones and blood, cut it into small pieces, add liquid nitrogen at a weight ratio of 1:5, and grind it repeatedly for 3 times to obtain bone marrow powder, and store it in a low-temperature refrigerator for later use;

[0045] b. Take 3 equal portions of 100 g of the pulverized bone marrow sample in step a. In each sample, the ratio of solid to liquid is 1:5, and the mass fraction is added respectively to 0.5%, 2.5% and 5% sodium chloride solution for extraction. Put it on an intensive constant temperature heating magnetic stirrer at a temperature of 40°C and repeatedly extract it twice until no sediment is found under the flat bottom. The reflux time is 3 hours. Filter the extract, add sodium chloride solution to the unfiltered part, and filter , combined filtrate;

[0046] C, the merging filtrate obtained by step b is degreased with a material-to-liquid ratio of 1:4 petr...

Embodiment 3

[0050] Buffer extraction:

[0051] a. Take fresh bone marrow, wash it 3 times, remove the bone and blood, cut it into small pieces, add liquid nitrogen to pulverize according to the weight ratio of 1:5, and pulverize it repeatedly 4 times to obtain bone marrow pulverized powder, and store it in a low-temperature refrigerator for later use;

[0052] b. Take 100 g of the crushed bone marrow powder obtained in step a, add tris hydrochloric acid with a weight ratio of 1:10, 0.1 mol / L, pH=8.4 buffer solution, and set an intensive constant temperature heating magnetic force at a temperature of 40°C Reflux on the mixer and extract repeatedly 3 times until no precipitate is seen to obtain the total extract;

[0053] c, the total extraction that step b obtains is degreased by the n-hexane of 1:6 according to the solid-liquid ratio, repeats 2-3 times, and collects the lower layer extract;

[0054] d. Centrifuge the lower layer extract obtained in step c at 12,000 rpm at 4° C. for 10 mi...

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Abstract

The invention relates to a preparation method and the application of bone marrow protein. The method comprises the following steps: adding fresh bone marrow in bones of cattle, sheep, horses, camels or animals in other animal slaughterhouses as raw materials; separately extracting protein in the fresh bone marrow subjected to water cleaning and pretreatment by using a trihydroxymethyl aminomethane hydrochloric acid buffer solution and a sodium chloride solution, wherein a lipid component is extracted by petroleum ether or normal hexane and no lipid component is left; then concentrating part of a water extract and carrying out dialysis desalting; using a modern spray drying technology or carrying out freeze drying and refining to obtain the bone marrow protein. The bone marrow protein is verified to have bacteria and fungus resisting activities, wide microorganism resisting activity and the functions of enhancing humoral immunity and general immunity, and is rich in pure nature-derived protein which is easily digested and absorbed by a human body; the aims of changing wastes into valuables and sufficiently utilizing resources are achieved while nutrition of the bone marrow protein is retained; the bone marrow protein is used as a natural antibacterial agent, a food additive or a medicine, and also can be used for food and medicine industries.

Description

technical field [0001] The invention relates to a method for preparing bone marrow protein and its application. It is a protein with antibacterial activity prepared by using the bone with rich resources in Xinjiang as a raw material. It has biological activities such as antibacterial and antifungal, and has good applications in the fields of medicine and food. prospect. Background technique [0002] Animal husbandry is the most distinctive traditional industry in Xinjiang, an important part of Xinjiang's agriculture, a pillar industry of Xinjiang's agricultural and rural economy, and an important foundation for the survival and development of people of all ethnic groups. Xinjiang has 48 million hm of usable grassland 2 , accounting for 23% of the total area of ​​the country's available natural pastures; with 4.1246 million hm of arable land 2 , crops and fodder resources are abundant; people of all ethnic groups have a long tradition and rich experience in animal husbandry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/30A61K38/02A61P31/04A61P31/10A23L33/18
CPCA61K38/00C07K1/145C07K1/30
Inventor 阿布力米提·伊力阿吉艾克拜尔·艾萨帕尔哈提·柔孜
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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