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A kind of preparation method of chickpea polypeptide part and application thereof

A chickpea polypeptide, chickpea technology, applied in the preparation method of peptides, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve problems such as toxic and side effects, achieve antioxidant and hypoglycemic activity, Enhance humoral immunity and systemic immunity, simple equipment effect

Active Publication Date: 2020-04-10
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its toxic and side effects, synthetic antioxidants are controlled by the governments of various countries to prevent excessive addition.

Method used

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  • A kind of preparation method of chickpea polypeptide part and application thereof
  • A kind of preparation method of chickpea polypeptide part and application thereof
  • A kind of preparation method of chickpea polypeptide part and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of chickpea watercress polypeptide powder:

[0043] a. Take the watercress after germination and tooth removal of the chickpea, rinse it with water 3 times until it is completely clean, filter with gauze respectively, combine the cleaned chickpea and watercress, dry at low temperature, control the temperature at 40 ° C, and drench the dried chickpea and watercress. Douban was pulverized by a pulverizer, and the pulverized particle size was 200 mesh. According to the material-liquid weight ratio of 1:5, petroleum ether was added, stirred at room temperature for 1 hour, and repeatedly extracted 3 times until no oil was extracted, suction filtration, and combined Precipitation, naturally dried until there is no petroleum ether solvent residue, to obtain degreasing chickpea flour;

[0044] b. Add 50% edible ethanol to the fully defatted chickpea flour in step a, with a solid-liquid ratio of 1:5, stir and extract at room temperature for 2 hours, extract 3 times e...

Embodiment 2

[0048] a. Take the chickpea dregs after extracting the small molecular compounds with a dry organic solvent, pulverize, the pulverized particle size is 200 mesh, add petroleum ether according to the material-liquid weight ratio of 1:5, stir at room temperature for 2 hours, and repeatedly extract 4 times Until no oil is extracted, suction filtration, combined precipitation, and natural drying until no petroleum ether solvent remains to obtain degreasing chickpea flour;

[0049]b. Add 50% edible ethanol to the fully defatted chickpea flour in step a, with a solid-liquid ratio of 1:5, stir and extract at room temperature for 3 hours, extract 4 times each time, filter under reduced pressure, and combine Extracting solution, separating impurities, and obtaining crude polypeptide extraction solution for subsequent use;

[0050] c. Dilute the crude polypeptide extraction solution obtained in step b to 20% ethanol concentration with distilled water, and apply 20% ethanol to the dilute...

Embodiment 3

[0053] Preparation of chickpea watercress polypeptide powder:

[0054] a. Take the watercress after germination and tooth removal of the chickpea, rinse it with water 3 times until it is completely clean, filter with gauze respectively, combine the cleaned chickpea and watercress, dry at low temperature, control the temperature at 40 ° C, and drench the dried chickpea and watercress. Douban was pulverized by a pulverizer, and the pulverized particle size was 200 meshes. Petroleum ether was added according to the material-liquid weight ratio of 1:5, stirred at room temperature for 2 hours, repeatedly extracted 4 times until no oil was extracted, suction filtered, and combined Precipitation, naturally dried until there is no petroleum ether solvent residue, to obtain degreasing chickpea flour;

[0055] b. Add 50% edible ethanol to the fully defatted chickpea flour in step a, with a solid-liquid ratio of 1:5, stir and extract at room temperature for 3 hours, extract 4 times each ...

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Abstract

The invention relates to a preparation method and application of a chickpea polypeptide part. The chickpea polypeptide part is chickpea polypeptide powder prepared by crushing and degeasing chickpeas or chickpeas residues obtained after extracting small molecule compounds with an organic solvent, extracting with ethanol, purifying with C18 reversed-phase column chromatography, preparing total polypeptide part with an ultrafiltration membrane technology, refining with a modern spray drying technology or freeze drying technology. Through verification, the chickpea polypeptide powder part has antibacterial and antifungal activities, has wide antimicrobial activity and a certain antioxidant activity and hypoglycemic activity, has the effects of enhancing the humoral immunity and general immunity and is enriched in pure natural plant source polypeptides and proteins which are easily digested and absorbed by the human body. The chickpea polypeptide powder part has the characteristics that the chickpea polypeptide proteins are remained, and the aims of changing the wastes into valuable materials and fully utilizing the resources are achieved; and moreover, the chickpea polypeptide powder serves as natural antibacterial agents, food additives or medicines and can be applied to the food and medicine industries.

Description

technical field [0001] The invention relates to a preparation method and application of a chickpea polypeptide part, which is a polypeptide part with antibacterial activity prepared by using chickpea watercress and chickpea dregs as raw materials, and has biological activities such as antibacterial, antifungal, etc. , The food field has a good application prospect. Background technique [0002] The discovery of antibiotics was a milestone in the history of human medicine. However, due to the abuse of antibiotics, the emergence of drug-resistant bacteria from time to time, and the frequency of emergence exceeds the development speed of new antibiotics, making many once highly effective antibiotics ineffective, so as to bring many new treatments to infectious diseases. The use of traditional antibiotics is facing severe challenges. It has become a development trend to research and apply new antibacterial agents that are non-toxic, pollution-free and drug-resistant to replace...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/20A23L33/18A61K38/16A61P31/04A61P31/10
Inventor 阿布力米提·伊力阿吉艾克拜尔·艾萨艾合米丁·外力肖凯提·萨利赫
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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