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Surface plasma resonance and mass spectrum combined device and application method thereof

A surface plasma and combined technology, which is applied in the field of online combined ion resonance and mass spectrometry devices, can solve the problems of low ionization degree, complicated operation process and intolerance, etc., and achieves strong ionization ability and sample signal. Lifting, well-tolerated effect

Inactive Publication Date: 2017-06-13
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

1. It can only be used offline, that is, the analysis solution of surface plasmon resonance cannot be detected by mass spectrometry at the same time as surface plasmon resonance analysis
At present, there is no report that MALDI technology can be used for real-time and direct analysis of the analysis solution in the process of surface plasmon resonance analysis, but only after the surface plasmon resonance analysis process is completed, the analysis solution can be collected or mass spectrometry detection for the analysis chip
2. The operation process is complicated
1. Although it is sometimes named online combination, it is not actually realized directly in real time, and usually requires additional enrichment, elution and other steps or equipment
2. There are restrictions on the properties of samples and solutions
ESI ion sources cannot tolerate solutions containing high concentrations of non-volatile salts, so there are limitations to the analysis system of surface plasmon resonance
Although the interface technology using the DART source realizes the real-time direct online coupling of surface plasmon resonance and mass spectrometry and can tolerate the presence of non-volatile salts to a certain extent, due to the limitation of the ionization principle of the DART source itself, the sample types it is applicable to Limited, low degree of ionization for many samples, affecting the detection sensitivity of the entire coupled system and analytical method

Method used

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  • Surface plasma resonance and mass spectrum combined device and application method thereof
  • Surface plasma resonance and mass spectrum combined device and application method thereof
  • Surface plasma resonance and mass spectrum combined device and application method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1: Using the surface plasmon resonance-mass spectrometry device based on the DBDI ion source of the present invention, a surface plasmon resonance-mass spectrometry experiment was performed to detect the interaction between human serum albumin and acetaminophen and perform mass spectrometry data in real time collection. Specific steps are as follows.

[0029] (1) Surface modification of the surface plasmon resonance detection chip. The detection chip used is a transparent glass plate coated with gold film. After installing the clean detection chip into the surface plasmon resonance instrument, flow the following solutions on the surface of the chip (gold-plated surface) at a flow rate of 0.2mL / min in sequence: A, 4mmol / L 3-mercaptopropionic acid aqueous solution, flow for 60min; B , EDC and NHS mixed aqueous solution, wherein EDC (N-(3-(dimethylamino)propyl)-N'-ethylcarbodiimidehydrochloride) is 12.5g / L, NHS (N-hydroxysuccinimide) is 2.5g / L, circulated for 20m...

Embodiment 2

[0033] Example 2: Using the surface plasmon resonance-mass spectrometry device based on the DBDI ion source of the present invention, performing a surface plasmon resonance-mass spectrometry experiment, detecting the interaction between human serum albumin and hippuric acid and collecting mass spectrometry data in real time . Specific steps are as follows.

[0034] (1) Surface modification of the surface plasmon resonance detection chip. The detection chip used is a transparent glass plate coated with gold film. After installing the clean detection chip into the surface plasmon resonance instrument, flow the following solutions on the surface of the chip (gold-plated surface) at a flow rate of 0.2mL / min in sequence: A, 4mmol / L 3-mercaptopropionic acid aqueous solution, flow for 60min; B , EDC and NHS mixed aqueous solution, wherein EDC (N-(3-(dimethylamino)propyl)-N'-ethylcarbodiimidehydrochloride) is 12.5g / L, NHS (N-hydroxysuccinimide) is 2.5g / L, circulated for 20min; C, hu...

Embodiment 3

[0038] Embodiment 3: Contrast the surface plasmon resonance-mass spectrometry device based on the DBDI ion source of the present invention and the document "Interface for Online Coupling of Surface Plasmon Resonance to DirectAnalysis in Real Time Mass Spectrometry (Zhang Y., Li X., Nie H ., Yang L., Li Z., Bai Y., Niu L., Song D., Liu H. Analytical Chemistry 2015,87,6505-6509)" based on the implementation of direct analytical ion source (DART source) Surface plasmon resonance-mass spectrometry device, the signal intensity results graph when analyzing two samples of acetaminophen and metronidazole. Specific steps are as follows.

[0039] (1) Device construction and experimental preparation based on DBDI source. The tested substances are acetaminophen and metronidazole, respectively dissolved in PBS buffer (phosphate buffered saline, containing 10mmol / L sodium phosphate, 138mmol / L sodium chloride, 2.7mmol / L potassium chloride, pH 7.4 ), the concentration is 20mmol / L. The DBDI...

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Abstract

The invention discloses a surface plasma resonance and mass spectrum combined device and an application method thereof. By the surface plasma resonance and mass spectrum combined device, real-time online combination of the surface plasma resonance technology and the mass spectrum technology is achieved in analytical chemistry. The surface plasma resonance and mass spectrum combined device is characterized in that a dielectric barrier discharge ion (DBDI) source is used as the ion source of a mass spectrometer, the DBDI source is placed at the front end of the inlet of the mass spectrometer, and the outlet of the DBDI source is allowed to face the inlet of the mass spectrometer; the analyzing solution of a surface plasma resonance instrument is introduced into a spray needle; the spray needle is arranged between the DBDI source and the inlet of the mass spectrometer, and a spray direction faces a DBDI source-mass spectrometer connecting line. When the surface plasma resonance and mass spectrum combined device works, the analyzing solution of the surface plasma resonance instrument is atomized by the spray needle, ionized by the DBDI source and introduced into the mass spectrometer for analysis, and surface plasma resonance data and mass spectrum data can be acquired at the same time in the same analysis.

Description

technical field [0001] The invention relates to a combined method of surface plasmon resonance and mass spectrometry in analytical chemistry, in particular to a device for realizing online combined use of surface plasmon resonance and mass spectrometry, and a method for building and using the device. Background technique [0002] Surface plasmon resonance (SPR) technology is an analytical method for detecting the interaction of substances, and is usually used for the analysis of intermolecular interactions (such as protein interactions, protein-drug small molecule interactions, etc.). In the analysis process, one of the reactants of the interaction reaction is modified on the surface of the detection chip of the surface plasmon resonance analysis instrument through covalent bonds, electrostatic interactions, and intermolecular forces, and then the other (or several) The reactants dissolve and flow across the surface of the detection chip. When an interaction reaction occurs...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 刘虎威白玉张一丁
Owner PEKING UNIV
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