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High-performance liquid chromatography for chiral analysis of nicotine in smokeless tobacco products

A high-performance liquid chromatography and smokeless tobacco technology, applied in the field of chiral analysis high-performance liquid chromatography, can solve the problems of complex processing steps and inability to meet the detection of a large number of samples, and achieve high sensitivity, reduce pollution, and short analysis time Effect

Inactive Publication Date: 2017-06-13
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

[0003] At present, the chiral analysis of nicotine is mainly studied by gas chromatography. For example, the patent application number 201610663534.1 "A Method for Chiral Analysis of Nicotine in Cigarette Tobacco" has complicated pretreatment steps and a long detection time. Up to 140min, which cannot meet the detection of a large number of samples. At present, there is no high performance liquid chromatography method for the chiral analysis of nicotine in smokeless tobacco products

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  • High-performance liquid chromatography for chiral analysis of nicotine in smokeless tobacco products
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  • High-performance liquid chromatography for chiral analysis of nicotine in smokeless tobacco products

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Embodiment Construction

[0014] The present invention is further described by the following specific examples, but does not limit the present invention.

[0015] 1. Instruments and Reagents

[0016] Agilent 1260 high performance liquid chromatograph equipped with UV detector (Agilent, USA), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), high-speed grinder (Wuhan Yincai Technology Co., Ltd.), Coleparmer Votex-Genie, USA Oscillator, Germany SIGMA 3-30K-high-speed bench-top refrigerated centrifuge. Hexane (chromatographically pure), sodium hydroxide (analytical pure), methanol (chromatographically pure), S-(-)-nicotine standard (CAS: 54-11-5), R-(+)-nicotine standard Products (CAS: 25162-00-9).

[0017] 2. Preparation of standard solution

[0018] Weigh about 100.0 mg of S-(-)-nicotine standard, dilute it with n-hexane and methanol solution (98:2, V / V) into a 50 mL brown volumetric flask, and prepare a concentration of about 2.0 mg / mL of S-(-)-nicotine standard stock solution; weigh ...

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Abstract

The invention discloses a high-performance liquid chromatography for chiral analysis of nicotine in smokeless tobacco products. The high-performance liquid chromatography is characterized by including drying smokeless tobacco product samples prior to grinding and sieving, impregnating the sieved sample in a sodium hydroxide solution, extracting through methyl tertiary butyl ether and a methanol solution, purifying an extraction solution by matrix solid-phase dispersion-extraction, analyzing by a chiral column-normal-phase liquid chromatography ultraviolet detector, and quantitatively determining ratios of S-(-)- nicotine and R-(+)- nicotine in the smokeless tobacco products through a peak-area normalization method. The high-performance liquid chromatography for chiral analysis of the nicotine in the smokeless tobacco products has the advantages that a sample extraction and purification method is simple and efficient, contamination of a sample solution to a chromatographic column can be reduced, a liquid-phase detection method is high in sensitivity and short in analysis method, and accordingly the high-performance liquid chromatography can meet chiral analysis requirements of the nicotine in big-batch smokeless tobacco products.

Description

Technical field [0001] The invention relates to a method for determining the chemical components of smokeless tobacco products, in particular to a high performance liquid chromatography method for chiral analysis of nicotine in smokeless tobacco products. Background technique [0002] Nicotine, also called nicotine, is an alkaloid found in Solanaceae plants and an important component of tobacco. The nicotine molecule contains a chiral center, that is, the 2 carbon atom on the tetrahydropyrrole ring, so nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. The two enantiomers of nicotine have completely different contents, metabolic mechanisms and physiological properties. Therefore, chiral analysis of nicotine in smokeless tobacco products is required. [0003] At present, the chiral analysis of nicotine is mainly carried out by gas chromatography. For example, the patent application number 201610663534.1 "A Chiral Analysis Method for Nicotine in Cigarette Tobacco" has...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/042G01N2030/062
Inventor 张洪非庞永强付亚宁朱风鹏罗彦波姜兴益李翔宇胡少东陈欢韩书磊刘彤侯宏卫
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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