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A Convergence Chromatography Tandem Mass Spectrometry Method for Chiral Analysis of Nicotine in Jieyanling

A technology of convergence chromatography and tandem mass spectrometry, applied in the directions of analysis of materials, material separation, instruments, etc., can solve the problems such as nicotine in quit smoking spirit, can not meet the detection of a large number of samples, complex processing steps, etc., and achieve high sensitivity and specificity. Good sex, avoid false positive effect

Active Publication Date: 2019-04-12
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

[0003] At present, the chiral analysis of nicotine is mainly studied by gas chromatography. For example, the patent application number 201610663534.1 "A Method for Chiral Analysis of Nicotine in Cigarette Tobacco". It can reach 140min, which cannot meet the detection of a large number of samples. At present, there is no method for chiral analysis of nicotine in Jieyanling

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  • A Convergence Chromatography Tandem Mass Spectrometry Method for Chiral Analysis of Nicotine in Jieyanling
  • A Convergence Chromatography Tandem Mass Spectrometry Method for Chiral Analysis of Nicotine in Jieyanling
  • A Convergence Chromatography Tandem Mass Spectrometry Method for Chiral Analysis of Nicotine in Jieyanling

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Embodiment Construction

[0015] The present invention is further described by the following specific examples, but does not limit the present invention.

[0016] 1. Instruments and Reagents

[0017] ACQUITY UPC2 ultra-high performance convergence chromatograph (Waters, USA), 6410B triple quadrupole mass spectrometer (Agilent, USA), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), high-speed pulverizer (Wuhan Yincai Technology Co., Ltd.), ColeparmerVotex-Genie vortex oscillator in the United States, and SIGMA 3-30K-high-speed desktop refrigerated centrifuge in Germany. MTBE (chromatographically pure), sodium hydroxide (analytical pure), formic acid (chromatographically pure), methanol (chromatographically pure), S-(-)-nicotine standard (CAS: 54-11-5 ), R-(+)-nicotine standard (CAS: 25162-00-9).

[0018] 2. Preparation of standard solution

[0019] Weigh about 100.0 mg of S-(-)-nicotine standard substance, dilute it with methanol solution to a 50 mL brown volumetric flask, and ...

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Abstract

The invention discloses a bonded-phase chromatography-tandem mass spectrometry for a chiral analysis on nicotine in a smoking cessation drug. The bonded-phase chromatography-tandem mass spectrometry is characterized by comprising the following steps: drying, smashing and sieving smoking cessation drug samples; after the samples are soaked by sodium hydroxide solution, extracting the solution with methyl tertiary butyl ether and a methanol solution; after matrix solid-phase dispersion extraction and purification are performed on extract liquor, making an analysis with two chiral columns by a tandem bonded-phase chromatography-tandem mass spectrometry; quantitatively detecting a proportion of S-(-)-nicotine and R-(+)-nicotine in the smoking cessation drug by adopting a peak area normalization method. According to the detection method provided by the invention, the extraction and purification methods of the samples are simple and efficient, so that the pollution of a sample solution to a chromatographic column can be reduced; the detection method of the bonded-phase chromatography-tandem mass spectrometry has the advantages that the sensitivity is high, the specificity is good, false positive can be eliminated, the analysis time is short, the requirement for the chiral analysis on the nicotine in a large batch of smoking cessation drug samples can be met.

Description

technical field [0001] The invention relates to a method for determining the chemical components of Jieyanling, in particular to a chiral analysis convergence chromatography tandem mass spectrometry method for nicotine in Jieyanling. Background technique [0002] Nicotine, also called nicotine, is an alkaloid found in plants of the nightshade family and an important component of tobacco. The nicotine molecule contains a chiral center, which is the 2-position carbon atom on the tetrahydropyrrole ring, so nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. The two enantiomers of nicotine have completely different contents, metabolic mechanisms and physiological properties. Therefore, it is necessary to carry out smoking quitting spirit (quitting smoking spirit is a smoke-like lighting product refined from more than 30 kinds of precious Chinese herbal medicines by using the medical principle of traditional Chinese medicine aroma, refreshing and resuscitation and a...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/72
CPCG01N30/06G01N30/7266G01N2030/062
Inventor 庞永强费婷张洪非姜兴益罗彦波吴达戚大伟李翔宇朱风鹏胡少东付亚宁陈欢韩书磊
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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