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Chiral analysis combined-phase chromatography-tandem mass spectrometry method of nicotine and nornicotine in electronic cigarette liquid

A technology of electronic cigarette liquid and convergence chromatography, which is applied in the direction of analyzing materials, material separation, and measuring devices, can solve the problems of easy false positives, poor qualitative ability, time-consuming and labor-intensive problems, and achieve chiral analysis, high sensitivity, The effect of short analysis times

Inactive Publication Date: 2017-12-15
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

[0003] At present, the methods reported in the literature for the determination of enantiomers of nicotine and nornicotine mainly include gas chromatography-mass spectrometry (GC-MS), multidimensional gas chromatography-mass spectrometry (MDGC-MS), and capillary electrophoresis (CE). ), etc., however, GC-MS and MDGC-MS need to be derivatized in the pretreatment process, which is time-consuming and laborious. CE only uses retention time for qualitative, relatively poor qualitative ability, prone to false positive results

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  • Chiral analysis combined-phase chromatography-tandem mass spectrometry method of nicotine and nornicotine in electronic cigarette liquid

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Embodiment Construction

[0015] The present invention is further described by the following specific examples, but does not limit the present invention.

[0016] 1. Instruments and Reagents

[0017] ACQUITY UPC2 ultra-high performance convergence chromatograph (Waters, USA), 6410B triple quadrupole mass spectrometer (Agilent, USA), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), high-speed pulverizer (Wuhan Yincai Technology Co., Ltd.), ColeparmerVotex-Genie vortex oscillator in the United States, and SIGMA 3-30K-high-speed desktop refrigerated centrifuge in Germany. Hexane (chromatographically pure), sodium hydroxide (analytical pure), formic acid (chromatographically pure), methanol (chromatographically pure), S-(-)-nicotine standard, R-(+)-nicotine standard, S -(-)-nornicotine standard, R-(+)-nornicotine standard.

[0018] 2. Preparation of standard solution

[0019] Weigh about 100.0 mg of S-(-)-nicotine, R-(+)-nicotine, S-(-)-nornicotine and R-(+)-nornicotine standard, ...

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Abstract

The invention discloses a chiral analysis combined-phase chromatography-tandem mass spectrometry method of nicotine and nornicotine in electronic cigarette liquid. The chiral analysis combined-phase chromatography-tandem mass spectrometry method of the nicotine and nornicotine in electronic cigarette liquid is characterized by comprising the following steps: soaking an electronic cigarette liquid sample into a sodium hydroxide solution, extracting by a normal hexane / methanol solution, performing matrix dispersion, solid-phase extraction and purification on the extracting liquid, performing analysis by a two-chiral-column series connection and combined-phase chromatography-tandem mass spectrometry method, and quantitatively detecting the proportion of S-(-)-nicotine, R-(+)-nicotine, S-(-)-nornicotine and R-(+)-nornicotine in the electronic cigarette liquid. The detection method and the sample extraction and purification method, which are provided by the method, are simple and efficient, pollution to a chromatographic column by the sample liquid can be reduced, the combined-phase chromatography-tandem mass spectrometry method detection method is high in sensitivity and high in specificity, false positive can be excluded, the analysis time is short, and chiral analysis of the nicotine and nornicotine in large-scale electronic cigarette liquid samples can be met.

Description

technical field [0001] The invention relates to a method for determining chemical components of electronic cigarette liquid, in particular to a chiral analysis convergence chromatography tandem mass spectrometry method for nicotine and nornicotine in electronic cigarette liquid. Background technique [0002] Nicotine and nornicotine are the main alkaloids present in Solanaceae plants and one of the important components of tobacco. Nicotine and nornicotine molecules contain a chiral center, so there are two enantiomers: S-(-)-nicotine, R-(+)-nicotine, S-(-)-nicotine and R -(+)-Nornicotine. The two enantiomers of nicotine and nornicotine have completely different contents, mechanisms of metabolism and physiological properties. Therefore, chiral analysis of nicotine and nornicotine in electronic cigarette liquid is required. [0003] At present, the methods reported in the literature for the determination of enantiomers of nicotine and nornicotine mainly include gas chromato...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 张洪非庞永强李翔宇罗彦波朱风鹏姜兴益
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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