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DNA structural element of ion concentration response and preparation and application thereof

A technology of ion concentration and structural elements, applied in the field of DNA structural elements, can solve problems such as restricting development, and achieve the effects of meeting production requirements, easy modification, and strong operability

Inactive Publication Date: 2017-06-13
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional electronic components have limited their ultimate development due to their own limitations, such as size, microstructure design and biocompatibility.

Method used

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  • DNA structural element of ion concentration response and preparation and application thereof
  • DNA structural element of ion concentration response and preparation and application thereof
  • DNA structural element of ion concentration response and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Synthesis of DNA triangular origami structure

[0033] The 208 booking chain is dissolved into Milliq water, so that the final concentration of each chain is 200nm. Mix the M13MP18 single-chain DNA (100nm) and the mixed 208 short chain (200nm) at a proportion of Moore to 1:10 to 1 × TAE-MG 2+ In the solution (TRIS), 40mm; acetic acid, 20mm; ethyleine tetraitine (EDTA), 2 mm; magnesium, 12.5mm; pH 8.0), of which the final concentration of M13MP18 single -chain DNA is the final concentration of M13MP18 single -chain DNA is to be 5 nm, the final concentration of the short chain is 50nm. Put the mixed solution in the PCR instrument, set the degree of reaction at 95 ° C for 3 minutes, then slowly cool down to 4 ° C, and the cooling rate is 0.2 ° C / 10s.

[0034] 2. Change the ion concentration and observe the changes in the DNA triangle structure with atomic power microscope

[0035] (1) 1 × ion concentration

[0036] Use 1 × TAE-MG 2+ The solution dilutes the prepared DNA tri...

Embodiment 2

[0042] 1. Synthesis of DNA triangular origami structure

[0043] The ordering chain for the composite rectangular origami structure is dissolved into Milliq water, so that the final concentration of each chain is 200nm. Mix the M13MP18 single-chain DNA (100nm) and the mixed booking nail short chain (200nm) at a proportion of Moore concentration to 1:10 at 1 × TAE-MG 2+ In the solution (TRIS), 40mm; acetic acid, 20mm; ethyleine tetraitine (EDTA), 2 mm; magnesium, 12.5mm; pH 8.0), of which the final concentration of M13MP18 single -chain DNA is the final concentration of M13MP18 single -chain DNA is to be 5 nm, the final concentration of the short chain is 50nm. Put the mixed solution in the PCR instrument, set the degree of reaction at 95 ° C for 3 minutes, then slowly cool down to 4 ° C, and the cooling rate is 0.2 ° C / 10s.

[0044] 2. Change the ion concentration and observe the changes in the DNA quadrilateral structure with the microscope of the atomic power

[0045] (1) 1 × io...

Embodiment 3

[0052] 1. Synthesis of DNA triangular origami structure

[0053] The 208 booking chain is dissolved into Milliq water, so that the final concentration of each chain is 200nm. Mix the M13MP18 single-chain DNA (100nm) and the mixed 208 short chain (200nm) at a proportion of Moore to 1:10 to 1 × TAE-MG 2+ In the solution (TRIS), 40mm; acetic acid, 20mm; ethyleine tetraitine (EDTA), 2 mm; magnesium, 12.5mm; pH 8.0), of which the final concentration of M13MP18 single -chain DNA is the final concentration of M13MP18 single -chain DNA is to be 5 nm, the final concentration of the short chain is 50nm. Put the mixed solution in the PCR instrument, set the degree of reaction at 95 ° C for 3 minutes, then slowly cool down to 4 ° C, and the cooling rate is 0.2 ° C / 10s. Essence

[0054] 2. Change the ion concentration and observe the changes in the DNA triangle structure with atomic power microscope

[0055] (1) 1 × ion concentration

[0056] Use 1 × TAE-MG 2+ The solution dilutes the prepared...

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Abstract

The invention relates to a DNA structural element of ion concentration response, and a preparation and application thereof. The preparation method comprises the following steps: synthesizing a DNA triangular paper folding structure; synthesizing a DNA quadrilateral folding paper structure, changing an ion concentration, and observing the DNA structure variation by utilizing an atomic force microscope. The invention particularly relates to the DNA triangular folding paper structure and the DNA quadrilateral folding paper structure serving as elements, and the two DNA structures have reversible ion response functions. The invention provides two DNA two-dimensional folding paper structure of the ion concentration response. When the ion concentration of a solution is changed, the DNA structural element can have the reversible structural variation; the DNA structural element is precise and controllable in structure, easy in decoration and good in biological compatibility; and the preparation method is simple, high in operability, and capable of further meeting the production requirement of a biological chip and a nano device.

Description

Technical field [0001] The invention involves the DNA structure component of the ion concentration response, which specifically involves the DNA triangular origami structure and the DNA quadrilateral origami structure, and these two DNA structures have reversible ion response functions. The present invention belongs to the field of nano -biomaterials. Background technique [0002] The DNA origami structure is composed of a long DNA single chain and many short DNA single chains. Each short chain is regarded as a few segments, complementary to a certain part of the long chain. When the long chain and short chain are placed in the solution in the same proportion, each short chain will pull the several parts of the long chain like a booking of the book, so the configuration of this long chain Change changes. If the position of each short chain is designed for a targeted position, this long chain will eventually be folded to become a set graphic and become a DNA origami structure. [...

Claims

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Application Information

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IPC IPC(8): C12P19/34G01Q30/20G01Q60/24B82Y5/00B82Y40/00
CPCB82Y5/00B82Y40/00C12P19/34G01Q30/20G01Q60/24
Inventor 何丹农王萍刘婷徐艳陈益金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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