Isothermal PCR (Polymerase Chain Reaction) detector and detection method thereof

Abstract

The invention discloses an isothermal PCR detector and a detection method thereof. The isothermal PCR detector comprises: a power supply module, an incubation module, one or more detection modules, an ultraviolet irradiation module, an optical processing module, an optical signal acquisition module, and a CPU control calculation module. The analysis module and the human-computer interaction module, the present invention mainly solves the problem of slow detection speed of the isothermal PCR instrument through the multi-channel design, and realizes that sample incubation and detection can be completed in the same equipment by integrating the incubation module on the detector Problems, the quantitative calculation of detection results is realized through advanced optical sensors and perfect algorithms.

Description

technical field [0001] The invention relates to an isothermal PCR detector and a detection method thereof. Background technique [0002] At present, isothermal PCR detectors mostly use the method of visual observation to judge the test results, and the incubation and detection of most of the existing equipment need to be carried out separately. The incubation and detection of this equipment are completed in the same equipment, and there is no need for intermediate Human operation. [0003] Although the method of naked eye observation is simple and clear, the method of naked eye observation can only achieve qualitative analysis, not quantitative analysis. Moreover, the method of naked eye observation is seriously affected by personal level, and cannot accurately and intuitively reflect the real situation of the measurement results. In addition, because most of the current equipment adopts the method of incubation and detection separation, it is not convenient for rapid on-si...

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Problems solved by technology

[0003] Although the method of naked eye observation is simple and clear, the method of naked eye observation can only achieve qualitative analysis, and cannot complete quantitative analysis. Moreover, the method of naked eye observation is seriously affected by the personal level, and cannot accurately and intuitively reflect the real situation of the measurement results

In addition, because most of the current equipment adopts the method of incubation and detection separation, it is not convenient for rapid on-site detection

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