Preparation method of heavy metal polluted soil repairing agent
A technology of polluted soil and remediation agent, applied in the field of soil remediation, can solve the problems of large project scale and large investment funds, achieve low investment and operation costs, low investment costs, and improve the effect of immobilization
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[0032] 1. Source of bacteria:
[0033] Bacteria cultured by biochemical treatment of activated sludge from comprehensive wastewater in an electroplating industrial park were activated in a transfer slope and liquid medium, and stored in a refrigerator at 4°C.
[0034] 2. Medium:
[0035] (1) Liquid medium: beef extract 3g, peptone 5g, NaCl 2.5g, distilled water 1000mL.
[0036] (2) Solid medium: beef extract 3g, peptone 5g, NaCl 2.5g, agar 15g, distilled water 15g.
[0037] (3) Screening medium: beef extract 3g, peptone 5g, NaCl 2.5g, distilled water 1000mL, add a certain concentration of cadmium and copper solution.
[0038] 3. Acclimatization, isolation and purification of strains:
[0039] (1) Preparation of soil suspension:
[0040] Take 10g of the mud sample and place it in a conical flask filled with 90mL of sterile water, vibrate with a shaker, and dilute the soil suspension to 10 -3 、10 -4 、10 -5 spare.
[0041] (2) Water sample dilution:
[0042] Take the soi...
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