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Preparation method of bovine serum albumin/polydopamine composite micro-nanosphere

The technology of bovine serum albumin and micro-nano spheres is applied in the field of preparation of bovine serum albumin/polydopamine composite micro-nano spheres, which can solve the problems that the drug release performance of the albumin nano-sphere carrier is not easy to control and the water solubility is high, and achieves the Good biocompatibility, long release time, good stability

Active Publication Date: 2020-02-21
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high water solubility of albumin molecules, the drug release performance of albumin nanosphere carriers prepared by this method is not easy to control. How to make albumin nanoparticles have good stability in water and not dissolve under dilute conditions It is a difficult point in the current preparation technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for preparing bovine serum albumin / polydopamine composite micro-nanospheres, comprising the following steps: adding dopamine hydrochloride solution into a stirred alcohol ammonia solution, then adding bovine serum albumin aqueous solution and ethanol I, and continuing to stir for 10 hour; filter and dry to obtain bovine serum albumin / polydopamine composite micro-nanospheres; the preparation method of the bovine serum albumin aqueous solution is: 0.1g of bovine serum albumin is dissolved in 50mL of water; the amount of ethanol I 200mL; the preparation method of the alcohol ammonia is: in 100mL of water, add 0.3mL ammonia water and 20mL ethanol II solution to configure the alcohol ammonia solution; the mass percentage of the ammonia water is 25%; the dopamine hydrochloride solution The preparation method is: take 2 mg of dopamine hydrochloride and dissolve it in 5 milliliters of water to configure dopamine hydrochloride solution;

[0022] When the bovine serum al...

Embodiment 2

[0024]A method for preparing bovine serum albumin / polydopamine composite micro-nanospheres, comprising the following steps: adding dopamine hydrochloride solution into a stirred alcohol ammonia solution, then adding bovine serum albumin aqueous solution and ethanol I, and continuing to stir for 24 hour; filter and dry to obtain bovine serum albumin / polydopamine composite micro-nanospheres; the preparation method of the bovine serum albumin aqueous solution is: 0.1g of bovine serum albumin is dissolved in 50mL of water; the amount of ethanol I 400mL; the preparation method of the alcohol ammonia is: in 100mL of water, add 0.3mL ammonia water and 70mL ethanol II solution to configure the alcohol ammonia solution; the mass percentage of the ammonia water is 28%; the dopamine hydrochloride solution The preparation method is: take 8 mg of dopamine hydrochloride and dissolve it in 5 milliliters of water to configure dopamine hydrochloride solution;

[0025] When the bovine serum alb...

Embodiment 3

[0027] A method for preparing bovine serum albumin / polydopamine composite micro-nanospheres, comprising the following steps: adding dopamine hydrochloride solution into a stirred alcohol ammonia solution, then adding bovine serum albumin aqueous solution and ethanol I, and continuing to stir for 12 hour; filter and dry to obtain bovine serum albumin / polydopamine composite micro-nanospheres; the preparation method of the bovine serum albumin aqueous solution is: 0.1g of bovine serum albumin is dissolved in 50mL of water; the amount of ethanol I 300mL; the preparation method of the alcohol ammonia is: in 100mL of water, add 1.5mL ammonia water and 70mL ethanol II solution to configure the alcohol ammonia solution; the mass percentage of the ammonia water is 28%; the dopamine hydrochloride solution The preparation method is: take 2 mg of dopamine hydrochloride and dissolve it in 20 milliliters of water to configure dopamine hydrochloride solution;

[0028] When the bovine serum a...

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Abstract

The invention discloses a preparation method of a bovine serum albumin / polydopamine composite micro-nano sphere. The preparation method comprises the following steps: adding a dopamine hydrochloride solution into a stirred alcohol ammonia solution, then adding a bovine serum albumin aqueous solution and ethyl alcohol I and stirring; filtering and drying to obtain the bovine serum albumin / polydopamine composite micro-nano sphere. The preparation method disclosed by the invention has the benefits that the bovine serum albumin / poly dopamine composite micro-nano sphere is prepared by utilizing good biocompatibility and biodegradability of bovine serum albumin and through combining polydopamine; the composite micro-nano sphere has the controlled release performance for drugs and can be used as a drug controlled release carrier to control the release of doxorubicin hydrochloride and other water-soluble small molecule drugs. As the drug carrier, the bovine serum albumin / polydopamine composite micro-nano sphere prepared by the preparation method disclosed by the invention is large in drug loading capacity, long in slow release time for the drugs, high in release speed and good in stability, and can be preserved for a long time.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a method for preparing bovine serum albumin / polydopamine composite micro-nano spheres. Background technique [0002] Among the medical polymer carrier materials, new drug carrier materials occupy a very important position and have received extensive attention from biology, medicine, and polymer materials. During the use of drugs or biologically active substances, choosing a suitable carrier material will greatly improve the effectiveness of the drug. However, different drug carrier materials have different drug release properties. An ideal drug carrier should have good biocompatibility, biodegradability, physical and chemical and biological stability, and extremely low cytotoxicity without causing any tissue damage. Stimulation and reaction, non-toxic, non-teratogenic and carcinogenic. At the same time, the carrier material must have good biocompatibility and stability with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K47/34A61K9/50A61K31/704A61P35/00
CPCA61K9/5031A61K9/5052A61K31/704
Inventor 张红平丁剑平唐苹苹侯佳良
Owner SOUTHWEAT UNIV OF SCI & TECH
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