Preparation method of bovine serum albumin/polydopamine composite micro-nano sphere
A technology of bovine serum albumin and polydopamine, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, microcapsules, etc. Easy to control and other problems, to achieve the effect of long sustained release time, good biocompatibility and good stability
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Embodiment 1
[0022] A method for preparing bovine serum albumin / polydopamine composite micro-nanospheres, comprising the following steps: adding dopamine hydrochloride solution into a stirred alcohol ammonia solution, then adding bovine serum albumin aqueous solution and ethanol I, and continuing to stir for 10 hour; filter and dry to obtain bovine serum albumin / polydopamine composite micro-nanospheres; the preparation method of the bovine serum albumin aqueous solution is: 0.1g of bovine serum albumin is dissolved in 50mL of water; the amount of ethanol I 200mL; the preparation method of the alcohol ammonia is: in 100mL of water, add 0.3mL ammonia water and 20mL ethanol II solution to configure the alcohol ammonia solution; the mass percentage of the ammonia water is 25%; the dopamine hydrochloride solution The preparation method is: take 2 mg of dopamine hydrochloride and dissolve it in 5 milliliters of water to configure dopamine hydrochloride solution;
[0023] When the bovine serum al...
Embodiment 2
[0025]A method for preparing bovine serum albumin / polydopamine composite micro-nanospheres, comprising the following steps: adding dopamine hydrochloride solution into a stirred alcohol ammonia solution, then adding bovine serum albumin aqueous solution and ethanol I, and continuing to stir for 24 hour; filter and dry to obtain bovine serum albumin / polydopamine composite micro-nanospheres; the preparation method of the bovine serum albumin aqueous solution is: 0.1g of bovine serum albumin is dissolved in 50mL of water; the amount of ethanol I 400mL; the preparation method of the alcohol ammonia is: in 100mL of water, add 0.3mL ammonia water and 70mL ethanol II solution to configure the alcohol ammonia solution; the mass percentage of the ammonia water is 28%; the dopamine hydrochloride solution The preparation method is: take 8 mg of dopamine hydrochloride and dissolve it in 5 milliliters of water to configure dopamine hydrochloride solution;
[0026] When the bovine serum alb...
Embodiment 3
[0028] A method for preparing bovine serum albumin / polydopamine composite micro-nanospheres, comprising the following steps: adding dopamine hydrochloride solution into a stirred alcohol ammonia solution, then adding bovine serum albumin aqueous solution and ethanol I, and continuing to stir for 12 hour; filter and dry to obtain bovine serum albumin / polydopamine composite micro-nanospheres; the preparation method of the bovine serum albumin aqueous solution is: 0.1g of bovine serum albumin is dissolved in 50mL of water; the amount of ethanol I 300mL; the preparation method of the alcohol ammonia is: in 100mL of water, add 1.5mL ammonia water and 70mL ethanol II solution to configure the alcohol ammonia solution; the mass percentage of the ammonia water is 28%; the dopamine hydrochloride solution The preparation method is: take 2 mg of dopamine hydrochloride and dissolve it in 20 milliliters of water to configure dopamine hydrochloride solution;
[0029] When the bovine serum a...
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