Method for removing foam during simultaneous saccharification and fermentation of lignocellulose with high solid content

A lignocellulosic, high solid content technology, applied in fermentation, biofuel, etc., can solve problems such as ineffectiveness

Inactive Publication Date: 2017-06-09
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these defoaming methods commonly used in conventional liquid fermentation do not work well in fermentation systems containing high solids

Method used

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  • Method for removing foam during simultaneous saccharification and fermentation of lignocellulose with high solid content
  • Method for removing foam during simultaneous saccharification and fermentation of lignocellulose with high solid content

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Add 1200g of dry straw after dedusting and crushing and 600g of dilute sulfuric acid solution with 5% mass fraction into a pretreatment reactor equipped with a ribbon-type stirring paddle to carry out high-temperature pretreatment reaction. The reaction conditions are 175°C, 2.5% dilute sulfuric acid consumption, The stirring speed is 50 rpm, and the temperature rises to 175°C and reacts for 5 minutes. Take out the pretreated material from the reactor, take 1500g and add it to a 5L bioreactor with a ribbon stirring paddle, and turn on the stirring speed of 50 rev / min, after adjusting the pH value to 5.5 with a calcium hydroxide solution of 20% mass concentration, insert 90 g of solid detoxified seeds cultivated in stalks with an inoculum size of 10% by mass ratio, stop stirring after mixing evenly, and start Solid-state biodegradation process, add 750ml deionized water after 48 hours of detoxification, saccharify with 85g of Youtel cellulase (15FPU / g) at 50°C and 150rpm ...

Embodiment 2

[0021] Add 1200g of dry straw after dedusting and crushing and 600g of dilute sulfuric acid solution with 5% mass fraction into a pretreatment reactor equipped with a ribbon-type stirring paddle to carry out high-temperature pretreatment reaction. The reaction conditions are 175°C, 2.5% dilute sulfuric acid consumption, The stirring speed is 50 rpm, and the temperature rises to 175°C and reacts for 5 minutes. Take out the pretreated material from the reactor, take 1500g and add it to a 5L bioreactor with a ribbon stirring paddle, and turn on the stirring speed of 50 rev / min, after adjusting the pH value to 5.5 with a calcium hydroxide solution of 20% mass concentration, insert 90 g of solid detoxified seeds cultivated in stalks with an inoculum size of 10% by mass ratio, stop stirring after mixing, and start Solid-state biodegradation process, add 750ml deionized water after 48 hours of detoxification, saccharify with 85g of Youtel cellulase (15FPU / g) at 50°C and 150rpm stirrin...

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Abstract

The invention relates to a method for removing foam during simultaneous saccharification and fermentation of lignocellulose with high solid content. The method specifically comprises the steps of 1, processing a lignocellulose material to increase the conversion rate of cellulose during enzymolysis; 2, conducting synchronous saccharification and fermentation on the processed material under high solid content, wherein continuous stirring is conducted in the early stage of fermentation, stirring is slowed down or stopped when a bubble layer appears, fermentation is conducted in a low-speed stirring state or a standing state, bubble aggregation can be effectively avoided, and bubble release is facilitated; and 3, conducting rectification, dehydration and subsequent processing on ethyl alcohol fermentation broth obtained in step 2. The method has the advantages that by conducting alcoholic fermentation in a low-speed stirring state or a standing state, power consumption during fermentation is reduced, overflow caused by generation of a large amount of carbon dioxide is fundamentally avoided, and a basis is laid for industrialization of bio-refinery of lignocellulose.

Description

technical field [0001] The invention relates to the fields of biorefinery and bioenergy, in particular to a method for eliminating foam in synchronous saccharification and fermentation of lignocellulose with high solid content. Background technique [0002] The main process of lignocellulose biorefinery includes pretreatment, enzymatic hydrolysis, saccharification and fermentation, and product separation. At present, the development trend of cellulosic ethanol is to use high solid content for ethanol fermentation to reduce the energy consumption of the subsequent separation and purification process and the production cost of fuel ethanol. However, there are many problems in high-concentration lignocellulose ethanol fermentation, such as high viscosity and accumulation of air bubble layer. However, the carbon dioxide bubbles accumulated in the fermentation are always in the form of small bubbles under the action of stirring, and are not easy to release. This is not the resu...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P7/10
CPCY02E50/10
Inventor 鲍杰侯伟亮张建
Owner EAST CHINA UNIV OF SCI & TECH
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