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Polygalacturonan rhamnogalacturonan1 (PGRG1) composition

A technology of polygalacturonic acid and galacturonic acid, which is applied in the field of polygalacturonic acid-rhamnogalacturonic acid (PGRG1) composition, can solve the problem of huge changes in composition and potency, and changes in chemical composition , inappropriate use and other issues

Inactive Publication Date: 2017-05-31
PHARMAGENESIS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Herbal medicines contain multiple components, and raw material growth and harvesting conditions can cause wide variations in composition and potency, thus leading to unpredictable variations in the chemical composition of different batches of botanical preparations obtained, making these preparations unsuitable for clinical use

Method used

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  • Polygalacturonan rhamnogalacturonan1 (PGRG1) composition
  • Polygalacturonan rhamnogalacturonan1 (PGRG1) composition

Examples

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preparation example Construction

[0170] Preparation of PGRG1 composition

[0171] When preparing the isolated and purified PGRG1 polysaccharide composition, hot water is first used to extract Astragalus membranaceus for preparation. Typically, treated clean root flakes or segments are obtained by trimming dry roots, cleaning with water and a disinfectant solution such as 70% ethanol, cutting into thin slices, and drying under near-sterile conditions, the output is called Slice the roots. The hot water is usually no lower than 80°C, often no lower than 90°C, and in some embodiments, about 100°C. Furthermore, a number of extraction cycles can be used for substantive and / or optimal extraction of PGRG1 from the roots, typically at least three cycles of three hours at 100°C each. The step of preparing dry root crumbs is usually carried out under near-sterile conditions using clean equipment. Concentrate the hot water extract (such as by evaporation under vacuum at 60-70°C) to bring the root slices to a concentr...

example 1

[0229] Preparation of PGRG1 composition

[0230] Step 1, root slice processing

[0231] The dried Astragalus raw material (root) (220kg) is processed into root slices by aseptic cleaning with ultrafiltered water, the contaminated part is removed, soaked overnight in 70% ethanol, cut into slices with a thickness of 0.3-0.5mm, and placed in Dry in a near-sterile oven at 60-70°C. Obtain about 200kg of dried root flakes, and the loss on drying is less than 10%.

[0232] Step 2, extraction and purification of polysaccharide composition

[0233] The dried root flakes (200 kg) were extracted 3 times with ultrafiltered water at about 100° C. for 3 hours each time. Add 2,000L of water for the first extraction, 1,600L for the second, and 1,200L for the third. A total of 4,500 L of mixed aqueous extracts was obtained and concentrated using a vacuum concentrator system at 60-80°C to obtain a concentrate with a volume of approximately 200 L. 200 L of the extract was precipitated with 70...

example 2

[0248] Preparation of PGRG1-5K compositions

[0249] Step 1, root slice processing (referring to example 1 step 1)

[0250] Step 2, extraction and purification of polysaccharide composition

[0251] The dried root flakes (200 kg) were extracted 3 times with ultrafiltered water at about 100° C. for 3 hours each time. Add 2,000L of water for the first extraction, 1,600L for the second, and 1,200L for the third. A total of 4,500 L of mixed aqueous extracts was obtained and concentrated using a vacuum concentrator system at 60-80°C to obtain a concentrate with a volume of approximately 200 L. 200 L of the extract was precipitated with 70% ethanol to remove small water-insoluble molecules. The resulting precipitate is then suspended in water and precipitated using 30%-35% ethanol to remove low water soluble polysaccharides, proteins and other larger molecules. The supernatant was reprecipitated in 70% ethanol to recover the highly water soluble polysaccharide or PGRG1. The pre...

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Abstract

The present disclosure relates to polygalacturonan rhamnogalacturonan (PGRG1) compositions, as well as methods of making and methods of using said compositions in medicinally useful and pharmaceutically useful forms. Specifically, the present disclosure provides purified PGRG1 compositions isolated from roots of the Astragalus genus of plants, and more particularly from the species Astragalus membranaceus, as well as PGRG1 compositions having a weight average molecular weight of at least 40 kiloDaltons (kDa).

Description

technical field [0001] The present application relates generally to linking compositions and methods of converting natural linking materials into medically useful and pharmaceutically acceptable compositions comprising polygalacturonic-rhamnogalacturonic acid (PGRG1), and methods of making and methods of using the composition. Specifically, the present application provides PGRG1 compositions isolated and purified from the roots of plants of the genus Astragalus, and more particularly from Astragalus species, and PGRG1 compositions having a weight average molecular weight of at least 40 kilodaltons (kDa). Background technique [0002] Astragalus is a large genus (Leguminaceae) found widely distributed throughout the world as annual and perennial flowering herbs and small shrubs. Astragalus has been used for centuries in Chinese and Persian medicine and became increasingly popular in the United States in the 1980s. In China, Astragalus is one of the 50 essential herbs used i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/481A61K36/00A61P31/12A61P13/12A01N65/00
CPCA61K36/481A61K45/06A61K31/715A61K31/732A61P13/12A61P31/12A61P37/02A61P43/00A61P7/00A23L33/105A23V2002/00A61K2236/13A61K2236/15A61K2236/17A61K2236/33C08B37/0048
Inventor 约翰·H·马瑟安金华
Owner PHARMAGENESIS
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