Immunochromatography detection kit for simple herpes virus II type IgM antibody

Abstract

The invention belongs to the field of biological medicines, and particularly relates to an immunochromatography detection kit for a simple herpes virus II type IgM antibody. The kit is formed by assembling a reagent strip and a plastic card, wherein the reagent strip comprises a base plate; a reaction membrane with a detection line and a quality control line is adhered to the base plate; a colloidal gold pad and a sample pad are stacked and adhered to one end, which is close to the detection line, of the reaction membrane in sequence; an absorption pad is stacked and adhered to one end, which is close to the quality control line, of the reaction membrane; the detection line is coated with a simple herpes virus II type antigen; the quality control line is coated with a goat anti-mouse IgG antibody; and the colloidal gold pad is coated with a colloidal gold labeled mouse anti-human IgM monoclonal antibody. The kit disclosed by the invention can obviously reduce the influence of environmental factors (such as low temperature and high temperature) on a detection process and a detection result, and guarantee the sensitivity, the accuracy and the stability of the detection result.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an immunochromatographic detection kit for herpes simplex virus type II IgM antibody. Background technique [0002] Herpes Simplex Virus (HSV) belongs to the human herpesvirus α subfamily, is a DNA double-stranded virus, and humans are its only natural host. According to the different antigenic properties, it can be divided into herpes simplex virus type I (HSV-1) type and herpes simplex virus type II (HSV-2 type), and the gene homologous sequence between the two is about 50%. The HSV-1 type usually infects the parts above the waist such as the mouth, face, and eyes, while the HSV-2 type usually infects the parts below the genitals and the perianal area. The typical clinical manifestations of herpes are clustered blisters and pustules on the skin and mucous membranes. Sores and scabs. [0003] HSV-2 is the most common cause of genital herpes, which has become one of the ...

AI Technical Summary

Problems solved by technology

Cell culture and PCR methods have high requirements on experimental conditions such as experimental sites and personnel, and cannot be carried out in most laboratories.

Direct immunofluorescence or ELISA methods for antigen detection need to rely on corresponding equipment, and most of the reagents are imported and expensive

Serum antibody detection, such as the commonly used colloidal gold immunochromatography, is widely used due to its strong specificity, high sensitivity, and simple operation. However, the colloidal gold immunochromatography is greatly affected by environmental and human factors, such as When the temperature is low (such as winter), the chromatography speed is slow, and the reaction time needs to be prolonged, otherwise it is easy to cause missed detection; or it is placed in the air for a long time, resulting in moisture, or when the ambient humidity is too high, the capillary action on the membrane is strengthened, Dot film is easy to cause T line, C line to widen or even spread, affecting the detection process and results

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