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Method for preparing galacto-mannan-oligosaccharides (GMOS)

A technology of galactomannan and oligosaccharide, which is applied in the field of preparation of galactomannan oligosaccharide, can solve the problems of inability to rapidly swell and hydrate, high viscosity of the reaction system, waste of time and resources, etc., and achieves good particle solubility, The effect of saving enzymatic hydrolysis time and convenient operation

Active Publication Date: 2017-05-31
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Guar gum has good water solubility and thickening properties, but the original powder often has the following disadvantages: slow dissolution rate, unable to quickly swell and hydrate; difficult to control viscosity; poor thermal stability, etc.
Oxidative degradation is a commonly used method to reduce the viscosity of colloids in chemical degradation, but chemical reagents are easy to cause residues, and the reaction is more violent and difficult to control
Enzymatic reaction conditions are mild, but too high substrate concentration results in high viscosity of the reaction system, slow reaction time, and incomplete enzymatic hydrolysis, while low substrate concentration will cause waste of time and resources in industrial production

Method used

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  • Method for preparing galacto-mannan-oligosaccharides (GMOS)
  • Method for preparing galacto-mannan-oligosaccharides (GMOS)
  • Method for preparing galacto-mannan-oligosaccharides (GMOS)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Dissolve 1mmol of ascorbic acid and 30mmol of food-grade hydrogen peroxide in 1L of water, add 200g of guar gum after dissolving evenly, and react in the reactor at 40°C for 15min at a speed of 40-60r / min.

[0018] 2. Add catalase to the reaction solution at 3000 U / L, and react at 40°C for 15 minutes.

[0019] 3. After the reaction, refer to GB-T 23499-2016, and use the titanium salt colorimetric method to determine whether hydrogen peroxide remains in the reaction system.

[0020] 4. Adjust the pH of the reaction solution to 2.5 with hydrochloric acid, add acidic β-mannanase according to the amount of 200 U / g guar gum substrate for enzymolysis, and react at 50°C for 1 hour.

[0021] 5. Boil the enzymatic hydrolysis solution to kill the enzyme, then centrifuge at 4800rpm for 20min to remove the insoluble enzymatic hydrolysis residue.

[0022] 6. Concentrate the supernatant to 400mL by suspension steaming, add 1200mL 95% ethanol according to 3 times the volume of the...

Embodiment 2

[0026] 1. Dissolve 20mmol of ascorbic acid and 1.5mol of food-grade hydrogen peroxide in 50L of water, steam the jacket of the enzymolysis tank to preheat the water in the tank to 45-50°C, add 12.5kg of guar gum, and ferment the tank at 50 Stir the reaction at ℃ for 25min, and the rotation speed is 55-60r / min.

[0027] 2. Add catalase to the reaction solution at 3000U / L, stir and react at 50°C for 15min, and rotate at a speed of 55-60r / min.

[0028] 3. After the reaction is over, refer to GB-T 23499-2016, and use the titanium salt colorimetric method to determine whether hydrogen peroxide remains in the reaction system.

[0029] 4. Adjust the pH of the reaction solution to 2.5 with hydrochloric acid, add acidic β-mannanase according to the amount of 200U / g guar gum substrate for enzymolysis, and continue to stir and react for about 1 hour at 50°C. After the enzymatic hydrolysis, the temperature was raised to 90°C and kept for 10 minutes to inactivate the enzyme.

[0030] 5. ...

Embodiment 3

[0035] Comparative observation of the laxative effect of oligosaccharide prepared by the present invention technology and commercial oligosaccharide product:

[0036] The galactomannoligosaccharides of the present invention were prepared according to Example 1.

[0037] Dissolve 1400g of guar gum substrate in 7L of water, add 2mmol / L of ascorbic acid and 40mmol / L of hydrogen peroxide to degrade after dissolving evenly, and shake at 40°C for 0.5h. Add catalase to the reaction solution according to 10000U / L, shake and react at 40°C for 20min. The pH of the reaction solution was adjusted to 2.5 with hydrochloric acid, acid β-mannanase was added according to the amount of 200U / g guar gum substrate for enzymolysis, and the reactor was stirred at 50-55°C for 4-5h. Heat the reaction kettle to 90°C for 10 minutes to inactivate the enzyme, centrifuge at 4800rpm for 20min to remove insoluble enzymolysis residues, and concentrate the supernatant to 2L by suspension evaporation, add 6L o...

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Abstract

The invention provides a method for preparing galacto-mannan-oligosaccharides (GMOS). The method comprises the following steps: with guar gum as a substrate, compounding and degrading with hydrogen peroxide and ascorbic acid; removing residual hydrogen peroxide in the system by utilizing catalase, and adding commercial acidic beta-mannanase for performing enzymolysis; performing enzyme deactivation on hydrolysate, centrifuging to remove impurities, concentrating, precipitating to remove carbohydrates of large molecular weight by utilizing 3-5 times ethanol, performing alcohol precipitation on supernatant, performing suspended evaporation and concentrating to the solid content of more than or equal to 20%, performing spray drying, thereby obtaining GMOS dried powder with the molecular weight of less than 10000Da; and performing wet granulation on GMOS dried powder with 80%-95% thanol, thereby obtaining solid particles with the granularity of 40-100 meshes and high solubility.

Description

technical field [0001] The invention belongs to the technical field of biological product preparation, and in particular relates to a preparation method of galactomannan oligosaccharides. Background technique [0002] Guar (Cyamops tetragonooba (L.) Taub.) is a tropical leguminous herb widely planted in India, Pakistan and other places. Guar gum (Guar gum) is obtained by peeling the endosperm part of guar seeds after removing the germ, drying and pulverizing, adding water, carrying out pressurized hydrolysis, precipitating with 20% ethanol, drying and pulverizing after centrifugation. The viscosity is the highest among natural colloids, and it is one of the cheapest and most widely used hydrophilic colloids in the world. Its main chemical composition is galactomannan, the main chain is (1→4)-β-D-mannose unit, and the side chain is a single α-D-galactose with (1→6) bond with the main chain. The ratio of galactose to mannose is 1:1.8, which is about 1:2. Guar gum has good w...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/12C12P19/04C12P19/00
CPCC12P19/00C12P19/04C12P19/12C12P19/14
Inventor 牟海津付晓丹李蓉
Owner OCEAN UNIV OF CHINA
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