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Method for regulating composition and proportion of monosaccharide in lucid ganoderma exopolysaccharides

A technology of extracellular polysaccharides and Ganoderma lucidum cells, which is applied in the field of regulating the composition and ratio of monosaccharides in the exopolysaccharides of Ganoderma lucidum, and can solve problems such as difficult to effectively expand, difficult to achieve complete consistency of monosaccharide composition and ratio, and difficult polysaccharide components. , to achieve the effect of inhibiting biological activity, uniform structure and high biological activity

Active Publication Date: 2017-05-31
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production and secretion of edible fungal polysaccharides including Ganoderma lucidum polysaccharides is a very complicated process. It is difficult to obtain polysaccharide components with the same molecular weight between different culture batches. Even if polysaccharide components with the same molecular weight can be obtained, their It is also difficult to achieve complete consistency in the composition and ratio of monosaccharides
Many Ganoderma lucidum polysaccharides with different structures have been reported to show certain biological activities, but the above-mentioned inhomogeneity makes it difficult to effectively conduct basic and applied basic research on functional foods or food components. in-depth development

Method used

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  • Method for regulating composition and proportion of monosaccharide in lucid ganoderma exopolysaccharides
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  • Method for regulating composition and proportion of monosaccharide in lucid ganoderma exopolysaccharides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Ganoderma lucidum seed culture: take 0.5cm 2 Large and small bacterial clumps are inoculated into the basic medium of 80mL / 250mL Erlenmeyer flask, 150r·min -1 , Cultivate at 30℃ for 7 days.

[0028] Ganoderma lucidum fermentation culture: add 150ml culture medium to 500ml Erlenmeyer flask, initial pH 6.0, sterilize at 110°C for 20 minutes. The inoculation amount is 0.5g wet weight Ganoderma lucidum mycelium, 150r·min -1 , Cultivate at 30℃ for 7 days.

[0029] After the fermentation, the monosaccharide composition in the Ganoderma lucidum extracellular polysaccharide was determined. The results showed that the proportions of glucose, mannose and galactose in Ganoderma lucidum polysaccharides were 81.64%, 9.44% and 6.98%, respectively.

Embodiment 2

[0031] Ganoderma lucidum seed culture: take 0.5cm 2 Large and small bacterial clumps are inoculated into the basic medium of 80mL / 250mL Erlenmeyer flask, 150r·min -1 , Cultivate at 30℃ for 7 days.

[0032] Ganoderma lucidum fermentation culture: add 150ml culture medium to 500ml Erlenmeyer flask, add glucose-6-phosphate at 0.1g / L, initial pH 6.0, sterilize at 110°C for 20 minutes. The inoculation amount is 0.5g wet weight Ganoderma lucidum mycelium, 150r·min -1 , Cultivate at 30℃ for 7 days.

[0033] After the fermentation, the monosaccharide composition in the Ganoderma lucidum extracellular polysaccharide was determined. The results showed that when the added amount of glucose-6-phosphate was 0.1g / l, the proportions of glucose, mannose and galactose in Ganoderma lucidum polysaccharides were 69.47%, 16.66% and 8.89%, respectively.

Embodiment 3

[0035] Ganoderma lucidum seed culture: take 0.5cm 2 Large and small bacterial clumps are inoculated into the basic medium of 80mL / 250mL Erlenmeyer flask, 150r·min -1 , Cultivate at 30℃ for 7 days.

[0036] Ganoderma lucidum fermentation culture: add 150ml medium to 500ml Erlenmeyer flask, add 0.3g / L glucose-6-phosphate, initial pH 6.0, sterilize at 110°C for 20 minutes. The inoculation amount is 0.5g wet weight Ganoderma lucidum mycelium, 150r·min -1 , Cultivate at 30℃ for 7 days.

[0037] After the fermentation, the monosaccharide composition in the Ganoderma lucidum extracellular polysaccharide was determined. The results showed that when the added amount of glucose-6-phosphate was 0.3g / L, the proportions of glucose, mannose and galactose were 53.02%, 29.02% and 15.51%, respectively.

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Abstract

The invention discloses a method for regulating the composition and proportion of monosaccharide in lucid ganoderma exopolysaccharides, and belongs to the field of microbial fermentation. According to the method, glucose 6-phosphate is added to regulate the proportion of glucose, mannose and galactose in the lucid ganoderma exopolysaccharides, synthesis of lucid ganoderma exopolysaccharides with specific monosaccharide composition and proportion is realized, and the lucid ganoderma exopolysaccharides prepared by applying the method has a bioactivity for effectively inhibiting tumor cells and has an inhibition rate of over 80 percent. The method lays foundation for lucid ganoderma exopolysaccharides with relatively high bioactivity, uniform structure and controllable preparation.

Description

Technical field [0001] The invention relates to a method for regulating the composition and ratio of monosaccharides in Ganoderma lucidum extracellular polysaccharides, and belongs to the field of microbial fermentation. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is a valuable edible and medicinal fungus. In ancient my country, its fruiting body was called "Palaceae". The Ministry of Health has approved Ganoderma lucidum as a new food resource. Ganoderma lucidum polysaccharide is one of the main effective chemical substances of Ganoderma lucidum. It is a kind of polysaccharide or heteropolysaccharide macromolecular substance connected by β-(1,3) and β-(1,4) bonds based on glucose. The production and secretion of polysaccharides from edible fungi, including Ganoderma lucidum polysaccharides, is a very complicated process. It is difficult to obtain polysaccharides with the same molecular weight between different culture batches. Even if polysaccharides with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04A23L33/125
CPCA23V2002/00C12P19/04A23V2250/51
Inventor 丁重阳王琼艾连中李进伟苑畅汪瑞石贵阳
Owner JIANGNAN UNIV
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