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Brown cotton fiber procyanidine transfer-related GST protein gene and application thereof

A technology of proanthocyanidins and brown cotton, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of single fiber color, restricting the production and application of brown cotton, and unsatisfactory quality, and achieve the effect of improving plant color

Inactive Publication Date: 2017-05-31
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, compared with white cotton, the quality of brown cotton is not ideal, and there are problems such as single fiber color, uneven coloring, low color fastness and color saturation, which seriously restrict the production and application of brown cotton.

Method used

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  • Brown cotton fiber procyanidine transfer-related GST protein gene and application thereof
  • Brown cotton fiber procyanidine transfer-related GST protein gene and application thereof
  • Brown cotton fiber procyanidine transfer-related GST protein gene and application thereof

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Embodiment 1

[0026] 1. Materials

[0027] The methods used in the following examples are conventional methods unless otherwise specified. The primers used were synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd., and the sequencing was performed by Shanghai Sangon Bioengineering Technology Service Co., Ltd. PMD-18T, restriction Endonuclease BamHI and XbaI, T 4 ligase. Both RNAprep Pure Plant Kit and FastQuant RT Kit were purchased from TIANGEN Company. DNA gel recovery kit, plasmid extraction kit, and X-Gluc were purchased from Shanghai Sangon Bioengineering Technology Service Co., Ltd., and the methods were carried out according to the instructions.

[0028] 2. Method

[0029] 2.1 Cloning of GhTT19 and construction of eukaryotic expression vector

[0030] 2.1.1GhTT19 gene cloning

[0031] The total RNA of brown cotton leaves was extracted and reverse-transcribed into cDNA. Specific amplification primers GhTT19-F and GhTT19-R were designed, and the cDNA was used...

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Abstract

The invention discloses brown cotton fiber procyanidine transfer-related GST protein gene and application thereof. The gene has a nucleotide sequence shown as SEQ ID NO.1 and an amino acid sequence shown as SEQ ID NO.2. According to the invention, the gene is found to be capable of controlling a formation of fiber pigment and improving plant color and luster through gene coded sequence analysis and genetic transformation of Arabidopsis thaliana; thus, reference basis is provided for utilizing a gene engineering technology to genetically improve brown cotton color characters, and a novel path is provided for cultivating a new brown cotton variety with stable fiber pigment.

Description

technical field [0001] The invention relates to the technical field of plant molecular biology, in particular to a brown cotton GhTT19 gene and its application. Background technique [0002] Cotton is a plant of the genus Gossypium Malvaceae. It is not only an important fiber crop, but also an important oil crop, and it is also a raw material for textiles, fine chemicals, and important strategic materials. Brown cotton is a kind of colored cotton with natural brown fibers. Because of its natural color, it can avoid bleaching, disinfection, dyeing and other processes in the cotton textile production process. It has less pollution and is environmentally friendly and healthy. As the concept of environmental protection is deeply rooted in the hearts of the people, brown cotton caters to the needs of the market and has broad market prospects. [0003] However, compared with white cotton, the quality of brown cotton is not ideal, and there are problems such as single fiber color,...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N15/82C12N1/21C12N9/10A01H5/00
CPCC12N9/1088C12N15/8205C12N15/825C12Y205/01018
Inventor 高俊山许磊陈文孟艳蔡永萍林毅
Owner ANHUI AGRICULTURAL UNIVERSITY
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