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Method of cultivating biological flocs and application thereof in breeding litopenaeus vannamei

A technology of biological flocs and culture medium, applied in microorganism-based methods, biochemical equipment and methods, applications, etc., can solve problems such as low conversion rate, and achieve the effects of short cycle, strong vitality, and increased food intake

Inactive Publication Date: 2017-05-31
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conversion rate of microorganisms to residual bait and organic waste in traditional aquaculture systems is extremely low, only 5% to 7% of nitrogen and 4% to 6% of phosphorus are transformed by microorganisms, and the carbon and nitrogen of aquaculture water are well regulated. The conversion rate of microorganisms to residual bait feces will be significantly improved. If carbon sources are added to the aquaculture water according to C / N=8-10, both organic nitrogen and inorganic nitrogen can be used in the aquaculture system, and ammonia can be consumed net, with a concentration of 10mg / L of ammoniacal nitrogen can be completely removed within 5 hours

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of microbial preparation thalline for cultivating biofloc

[0045] Lactobacillus acidophilus (Lactobacillus acidophilus) PL7 preservation number CCTCC NO: M2013294 cultivation: the starting strains were cultured in the order of slant, liquid seeds, and liquid fermentation, and the slant and seed medium were conventional MRS medium; lactic acid bacteria fermentation was added with 0.5% low Polyfructose is used as an agent to promote the growth of lactic acid bacteria. Fermentation medium formula (W / V): molasses 6%, brown sugar 3%, yeast extract 0.03%, peptone 0.6%, oligosaccharides 0.5%, magnesium sulfate 0.05%, manganese sulfate 0.05% , calcium carbonate 0.5%; the fermentation condition is 37 ℃ static culture 36 ~ 48h, after the fermentation is finished, dilute 2 times, adjust the pH to 3 ~ 4, and add trehalose, vitamin C, fructooligosaccharides, mannitol, starch At least one of the dextrins, mixed uniformly at a ratio of 1% to 3%, and the measured...

Embodiment 2

[0048] Example 2 The test of rapidly cultivating biofloc

[0049] 1. Materials and methods

[0050] 1. Test product: the compound probiotics of embodiment 1.

[0051] 2. Test water body: The test water is taken from sand-filtered seawater.

[0052] 3. Test site: The test is carried out in a glass fiber cylinder of 80cm×100cm×100cm (length×width×height). The test tank was placed indoors under natural conditions near the window, and nano-aeration tubes were arranged in the same direction around the glass fiber tank, and placed at the height of 10CM and 50CM at the bottom of the tank respectively.

[0053] 4. Test method: 100 tails of Penaeus vannamei prawns with a length of 3 cm in 6 glass fiber tanks with specifications of 80 cm × 100 cm × 100 cm (length × width × height) were used for the test, of which 3 were the experimental group and 3 Mouth is the control group; the cycle is 30 days, and the experimental group adopts molasses to adjust the C / N ratio of the water body to...

Embodiment 3

[0062] Embodiment 3 Application test 1 of rapid cultivation biofloc of the present invention in prawn culture

[0063] 1. Test product: the compound probiotics of Example 1.

[0064] 2. Experimental prawns: The prawns from seedlings to 30 days in the test period are used throughout the whole process. Set up 5 experimental pools and 5 control pools, a cement pool with a volume of 5m×5m×1.5m and a water depth of 1.2m. Two rows of nano-aeration tubes are arranged around, and the heights are 10cm and 60cm from the bottom of the pool respectively.

[0065] 3. Test site: a prawn farm in Dongshan Island, Zhangzhou.

[0066] 4. Test method: during daily feeding, take the compound bacterial agent of Example 1 and sprinkle it in the breeding pond, and the daily usage and dosage: 50g of bacillus and yeast, 200mL of lactic acid bacteria liquid and 200g of corn cob powder are mixed and fermented, fermented overnight at 37°C and sprinkled; and Add diluted molasses to control the carbon-to...

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Abstract

The invention relates to a method of cultivating biological flocs and an application thereof in breeding litopenaeus vannamei and relates to cultivation of biological flocs. The cultivated biological flocs comprise bacillus pumilus, lactobacillus acidophilus and pichia guilliermondii. The method comprises the following steps: preparing a bacillus pumilus inoculant, a lactobacillus acidophilus inoculant and pichia guilliermondii powder; adopting a way of splashing bacillus pumilus powder, saccharomycetes powder and a lactic acid bacteria inoculant for a water body per mu every day, and arranging an nano aerating tube in the lower layer around a cultivation pond to aerate to form the flocs; adjusting the C / N weight of a cultivating water body and controlling the C / N at (12-14): 1; and after the biological flocs are cultivated, splashing the bacillus pumilus inoculant, pichia guilliermondii powder and a lactobacillus acidophilus inoculant to the water body per mu every day. The cultivated biological flocs can be applied to breeding litopenaeus vannamei.

Description

technical field [0001] The invention relates to the cultivation of biological flocs, in particular to a method for cultivating biological flocs and its application in the culture of Penaeus vannamei. Background technique [0002] The core of biofloc technology is to add carbon sources and probiotics to aquaculture water, increase the C / N ratio of water, and promote heterotrophic microorganisms to purify water quality and flocculate. The conversion rate of microorganisms to residual bait and organic waste in traditional aquaculture systems is extremely low, only 5% to 7% of nitrogen and 4% to 6% of phosphorus are transformed by microorganisms, and the carbon and nitrogen of aquaculture water are well regulated. The conversion rate of microorganisms to residual bait feces will be significantly improved. If carbon sources are added to the aquaculture water according to C / N=8-10, both organic nitrogen and inorganic nitrogen can be used in the aquaculture system, and ammonia can ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F3/34A01K61/59C12R1/07C12R1/23C12R1/84C02F103/20
CPCC02F3/34C02F2103/20C12N1/16C12N1/20Y02A40/81
Inventor 邵宗泽单大鹏李旭林剑辉王丽萍
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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