Lactobacillus paracasei n1115 freeze-dried powder starter and preparation method thereof

Abstract

The invention provides a lyophilizing protectant which enables lyophilizing matrix to exhibit unique loose spatial network structure; such structure provides spatial protective barrier for bacteria and helps the lyophilizing protectant the bacteria to play a role in anti-freezing protection. The invention also provides Lactobacillus paracasei N1115 lyophilized powder fermenting agent and a preparation method thereof; bacteria are enriched by pre-freezing and low-temperature high-speed centrifuging (5 DEG C and 8000 r / min) to obtain Lactobacillus paracasei N1115 concentrate, the yield of the bacteria acquired by the method is high, production efficiency is improved significantly, and production cost is lowered.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and relates to a freeze-dried protective agent and a Lactobacillus paracasei N1115 freeze-dried powder starter containing the freeze-dried protective agent; meanwhile, the invention also relates to a freeze-dried powder of the Lactobacillus paracasei N1115 Method of preparation of leavening agent. Background technique [0002] Lactobacillus paracasei N1115 is a lactic acid bacterium with probiotic function, which has been used in lactic acid bacteria drinks and instant probiotic powder. Probiotic freeze-dried powder products have the advantages of low production cost, strong vitality, good stability and long storage period. However, in the production of freeze-dried preparations of probiotics, there are disadvantages of high cell death rate and poor stability, resulting in low content of viable bacteria in the product, large dosage, poor fermentation effect, and high manufacturing and use...

AI Technical Summary

Problems solved by technology

The method pointed out in this patent application can be used for multiple species of probiotics. Since different species of microorganisms have unique metabolic and stress resistance characteristics, the general production process has different effects on different strains, such as the application of Lactobacillus paracasei When the N1115 freeze-dried powder starter is used, the yield of viable bacteria is low and the stability is poor

[0004] After the microorganisms are freeze-dried, the free water in the cells will be removed in a frozen state, and all life activities of the cells will stop and be in a dormant state, which can be stored for a long time, but the process of freeze-drying will cause damage, death and cell damage to the microbial cells. Inactivation of certain enzyme protein molecules, so it is necessary to add a lyoprotectant to reduce cell damage

In order to reduce cell damage, in the prior art, usually in the freeze-drying process, a protective agent is added, such as using glycerol as a protective agent when cryopreserving bacterial strains, providing effective protection for the freeze-drying process, but glycerol freeze-drying process Not easy to dry, can not achieve better protection effect

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