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Preparation method and application of yellow boletus active substance

An active substance, a technology of boletus serrata, applied in the field of preparation of active substance of boletus serrata, can solve the problems of unsatisfactory yield, long immersion method, low extraction rate of decoction method, etc., and achieves maintenance of antioxidant and extraction efficiency. High, easy-to-use effects

Active Publication Date: 2020-05-19
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the extraction rate of the decoction method is often low, and it is easy to destroy the active ingredient, while the maceration method takes a long time and the yield is not ideal.

Method used

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  • Preparation method and application of yellow boletus active substance
  • Preparation method and application of yellow boletus active substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Dry the boletus fruiting bodies to constant weight, crush them with a medicinal pulverizer, and pass through an 80-mesh sieve to obtain boletus powder; prepare an aqueous ethanol solution with a volume fraction of 45.78%, take 6.07 mL of the aqueous ethanol solution, and add 0.1 g boletus powder, add sodium hydroxide to a final concentration of 0.07mol / L, mix thoroughly in a mixing tube, and fix in an ultrasonic bath device; first, keep a constant temperature water bath at 40°C for 40 minutes; then turn on ultrasonic treatment, 500W ultrasonic frequency , treated for 9.94min, quickly cooled to room temperature in an ice bath, and adjusted the pH to 6.5; the filtrate was collected by suction filtration, and the filtrate was dried to obtain the active substance of Boletus cerevisiae.

Embodiment 2

[0025] Dry the boletus fruiting bodies to constant weight, crush them with a medicinal pulverizer, and pass through an 80-mesh sieve to obtain boletus powder; prepare an aqueous ethanol solution with a volume fraction of 45.78%, take 6.07 mL of the aqueous ethanol solution, and add 0.1 g boletus powder, add sodium hydroxide to a final concentration of 0.05mol / L, mix thoroughly in a mixing tube, and fix in an ultrasonic bath device; first, a constant temperature water bath at 40°C for 60 minutes; then turn on ultrasonic treatment, 400W ultrasonic frequency , treated for 15 minutes, quickly cooled to room temperature in an ice bath, and adjusted to pH 6.5; the filtrate was collected by suction filtration, and the filtrate was dried to obtain the active substance of Boletus cerevisiae.

Embodiment 3

[0027] Dry the boletus fruiting bodies to constant weight, crush them with a medicinal grinder, and pass through a 120-mesh sieve to obtain boletus powder; prepare an ethanol solution with a volume fraction of 40% ethanol, take 6.0 mL of the ethanol solution, and add 0.1 g boletus powder, add sodium hydroxide to a final concentration of 0.1mol / L, mix thoroughly in a mixing tube, and fix in an ultrasonic bath device; first 40°C constant temperature water bath for 60 minutes; then turn on ultrasonic treatment, 500W ultrasonic frequency and then treated for 10 minutes, quickly cooled to room temperature in an ice bath, and adjusted the pH to 7.0; the filtrate was collected by suction filtration, and the filtrate was dried to obtain the active substance of Boletus cerevisiae.

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PUM

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Abstract

The invention discloses a preparation method and an application of a chanterelle active substance. On the basis of an alcohol extraction technology, and under the assistance of alkali treatment and ultrasonic treatment, the active substance is extracted from a chanterelle fruiting body; and antioxidant, anti-cancer and bacteriostatic activities of the extracted chanterelle active substance can be reserved to the greatest extent. The preparation method provided by the invention is simple to operate and high in extraction efficiency, and the preparation method is applicable to large-scale industrial production.

Description

technical field [0001] The present invention relates to a preparation method of an active substance, more specifically, to a preparation method and application of an active substance of boletus flavus. Background technique [0002] Edible fungi are a class of large fungi that are rich in nutrients and can be eaten. Among them, boletus is a widely distributed wild edible fungus. Its wild fruiting bodies are rich in nutrients such as various amino acids, vitamins, mineral elements, proteins, and active polysaccharides, and are deeply loved by people. Traditional Chinese medicine believes that "medicine and food have the same origin, and food and medicine have both." Yellow boletus is not only a good food, but also has great medicinal value. At present, it has been found that Boletus cerevisiae has the functions of scavenging oxygen free radicals, anti-aging, clearing away heat and detoxification, and inhibiting bacteria and disinfection. Therefore, the preparation of the bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/07A61P39/06A61P35/00A61P31/04
CPCA61K36/07A61K2236/333
Inventor 李华斌徐冬平李莎周玥郑洁李娅
Owner SUN YAT SEN UNIV
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