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Application of perhexiline in treating stomach cancers and intestinal cancers

A perhexiline and gastric cancer technology, applied in the field of medicine, can solve problems such as no perhexiline reports

Active Publication Date: 2017-05-31
SUN YAT SEN UNIV CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are no reports on the application of perhexiline in other diseases such as gastric cancer and intestinal cancer

Method used

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  • Application of perhexiline in treating stomach cancers and intestinal cancers
  • Application of perhexiline in treating stomach cancers and intestinal cancers
  • Application of perhexiline in treating stomach cancers and intestinal cancers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The effect of peroxilin on gastric cancer and colorectal cancer cells and their tumor formation

[0031] 1. Experimental materials

[0032] (1) Medicine: Perxicillin is commonly used clinically as its maleate salt. In this example, perxicillin maleate is used as the experimental drug.

[0033] The chemical structure of pipexillin maleate is shown below:

[0034]

[0035] (2) Cancer cells:

[0036] Gastric cancer cells: human gastric cancer cells HGC27 and MGC803;

[0037] Colorectal cancer cells: human colon cancer cell HCT 116 and human colon adenocarcinoma cell DLD-1.

[0038] (3) A commercially available cell apoptosis kit.

[0039] 2. Experimental grouping

[0040] (1) Control group: blank control, that is, cancer cells are not treated with any drugs.

[0041] (2) Experimental group: Treat cancer cells with pipexillin maleate.

[0042] 3. Flow cytometry to detect the effect of peroxilin maleate on the apoptosis of gastric cancer and colorectal cancer cells

[0043] (1) We ...

Embodiment 2

[0060] Example 2 The effect of peroxilin on the proliferation of gastric cancer and colorectal cancer cells

[0061] 1. Experimental materials

[0062] (1) Medicine: Same as Example 1.

[0063] (2) Cancer cells: the same as in Example 1.

[0064] (3) A commercially available MTS kit.

[0065] 2. Detect the viability of HGC27 and MGC803 cells, as well as HCT 116 and DLD-1 cells treated with perxicillin maleate by MTT method

[0066] (1) Pour gastric cancer and colorectal cancer cells in a 96-well plate. After they adhere to the wall, add a medium containing 0μM, 5μM, 10μM, 20μM perxicillin maleate to treat the cells, and then treat the cells at 24h, 48h At 72h, the activities of HGC27 and MGC803 cells, HCT 116 and DLD-1 cells were detected by MTT method.

[0067] The specific method is as follows:

[0068] 1) After digestion of HGC27 and MGC803 cells, HCT 116 and DLD-1 cells in logarithmic growth phase with trypsin, take 4×10 3 The cells are spread evenly in a 96-well plate.

[0069] 2) Aft...

Embodiment 3

[0073] Example 3 The effect of perxirin on the metastasis of gastric cancer and colorectal cancer

[0074] 1. Experimental materials

[0075] (1) Medicine: Same as Example 1.

[0076] (2) Gastric cancer cells: same as in Example 1.

[0077] (3) Transwell cells with and without pre-coated Matrigel.

[0078] 2. Detect the effect of perhexiline maleate treatment on the invasion and migration of HGC27 and MGC803 cells, HCT116 and DLD-1 cells through the Transwell chamber

[0079] (1) Pour gastric cancer and colorectal cancer cells in a six-well plate. After they adhere to the wall, add 0μM, 3μM and 4μM (or 5μM) perxicillin maleate to treat gastric cancer cells for 48h. Add 0μM, 4μM and Intestinal cancer cells were treated with 6μM pipexillin maleate for 48 hours, and then the effects of the invasion and migration of HGC27 and MGC803 cells, HCT 116 and DLD-1 cells were detected by the Transwell chamber.

[0080] The specific method is as follows:

[0081] 1) After digesting HGC27 and MGC803 ce...

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PUM

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Abstract

The invention discloses an application of perhexiline in treating stomach cancers and intestinal cancers. After proofing by study, the perhxiline can obviously inhibit the growth, proliferation and coloning formation abilities of stomach cancer and colorectal cancer cells, induce the withering of the stomach cancer and colorectal cancer cells, inhibit the invasion and transfer abilities of the stomach cancer and colorectal cancer cells, and have obvious treatment effect on the stomach cancer and colorectal cancer, so that the perhxiline can be used as a medicine for treating the stomach cancer and intestinal cancer. The application has the advantages that a new application of the perhxiline is provided, the clinical application field of the perhxiline is widened, and a new chemical therapy medicine and a therapy path are provided for the therapy of stomach cancer and colorectal cancer.

Description

Technical field [0001] The invention belongs to the field of medical technology. More specifically, it relates to the application of perxicillin in the treatment of gastric cancer and intestinal cancer. Background technique [0002] Gastric cancer and bowel cancer are common gastrointestinal tumors worldwide, ranking first among various malignant tumors in my country, and are one of the main causes of death worldwide, and there are great difficulties in clinical treatment. [0003] Perhexiline is a calcium ion channel antagonist, which has the effect of inhibiting Ca2+ influx, can relax vascular smooth muscle, significantly dilate coronary arteries, increase coronary blood flow, and has a good effect on angina pectoris. It is mainly used in the treatment of angina pectoris. At the same time, it is also clinically used for ventricular arrhythmia, especially for patients whose other antiarrhythmic drugs are ineffective, this product often works. Further studies have found that pero...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4458A61P35/00
CPCA61K31/4458
Inventor 徐瑞华王运鞠怀强
Owner SUN YAT SEN UNIV CANCER CENT
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