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Method for micropropagation of arbuscular mycorrhizal fungi

A technology of arbuscular mycorrhizal fungi and inoculum amount, which is applied in horticultural methods, botanical equipment and methods, planting substrates, etc., can solve the problems of high technical difficulty and high atomization cost, and achieves high mycorrhizal infection rate and operation. Simple and efficient multiplication

Inactive Publication Date: 2017-05-31
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0010] The host plants pre-inoculated with arbuscular mycorrhizal fungi are placed in the air in an aerosol incubator, and then the roots are sprayed, that is, ultrasonically atomized nutrient solution is sprayed to the roots of the host plants every few seconds to meet the needs of the roots for water and nutrients , can produce a large number of fibrous roots containing arbuscular mycorrhizal fungi under soilless conditions. The advantages of this method are that the bacterial agent has high purity and light weight, but due to the high cost of atomization, and the parameters required by the atomization culture system for the nutrient solution Many, especially for the control of phosphorus and pH, so the technical difficulty also affects the technical application
[0011] Although arbuscular mycorrhizal fungi have good efficacy as bio-fertilizers, there is still a lack of a method for the proliferation of arbuscular mycorrhizal fungi suitable for large-scale industrial production

Method used

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  • Method for micropropagation of arbuscular mycorrhizal fungi
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  • Method for micropropagation of arbuscular mycorrhizal fungi

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Mix zeolite, pumice, vermiculite and peat with a particle size of less than 2.5mm at a volume ratio of 12:3:3:1. After mixing, sterilize with high-temperature hot water (100°C) and keep warm at 60°C for 3 hours; spread clean water on the ground and set up an insect-proof net, place a perforated pot with a height of 80 cm and a diameter of 50 cm on the ground, and then place the sterilized matrix in the pot.

[0042]Inoculate Glomus intraradices in the matrix and mix evenly, the inoculation amount is 5% of the mass of the matrix; in the early spring season, the matrix is ​​watered thoroughly, and corn, marigold and clover are sown successively in the matrix after inoculation; the density of the sowing 12 pieces / m 3 ; Cover a layer of white film on the top of the pot, and remove the film after emergence. After 1 week, remove the weak plants, leave 10 plants in the tank, and then apply 0.25 mg / m to the root zone 3 of spermidine. After 5 months of multiplication, remove ...

Embodiment 2

[0050] The results of the G.i mycorrhizal mycorrhizal agents inoculated on different substrates were compared, and the results are shown in Table 1.

[0051] Two kinds of cultivation substrates were used in the test: substrate 1 was mixed with zeolite, pumice, vermiculite and peat (particle size less than 2.5) in a volume ratio of 12:3:3:1, and substrate 2 was zeolite, vermiculite and peat (particle size was less than 2.5 ) were mixed at a volume ratio of 12:3:1 to inoculate arbuscular mycorrhizal fungi in different cultivation substrates by means of multiplication, compare their infection rates, and obtain arbuscular mycorrhizal fungal inoculants with good infection rates.

[0052] Table 1 Root infection rate of arbuscular mycorrhizal fungus propagation

[0053]

[0054] It can be seen from Table 1 that the mycorrhizal rate of the bacterial agent propagated by substrate 1 is significantly higher than that of substrate 2, so zeolite, pumice, vermiculite and peat (particle s...

experiment example 3

[0056] Effects of Polyamines on Mycorrhizal Infection Rate and Root Vitality

[0057] figure 1 It is a graph showing the effect of exogenous polyamines on AMF and root vigor of host plants, figure 2 It is the result graph of the effect of exogenous polyamines on the infection rate of AMF roots. As shown in the figure, adding AMF and Spd to the mixed matrix significantly increased the root activity of the host plant; adding Spd and AMF to the mixed matrix at the same time, compared with Gv, the root activity and infection rate of the host plant increased significantly . The above results indicated that Spd and AMF could increase the root activity of host plants, and Spd could significantly increase the mycorrhizal infection rate of AMF, thereby promoting the proliferation of the strain.

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Abstract

The invention relates to a method for micropropagation of arbuscular mycorrhizal fungi and belongs to the technical field of micropropagation of mycorrhiza fungi. The method for micropropagation of the arbuscular mycorrhizal fungi includes the following steps that firstly, the arbuscular mycorrhizal fungi are inoculated into a substrate to be cultivated, wherein the substrate comprises zeolite, pumice, vermiculite and peat; secondly, corn, marigold and clover are sequentially sown in the substrate in the first step, wherein the sowing density of each kind of plants is independently 8-12 seeds per square meter; thirdly, the sowing time of the corn serves as the reference, after the plants grow for 4-6 months, underground mycorrhizas are dried and smashed to be mixed with the substrate for cultivating the arbuscular mycorrhizal fungi. The method is high in mycorrhiza infection rate, high in fungus yield and good in micropropagation effect.

Description

technical field [0001] The invention relates to the technical field of mycorrhizal fungus propagation, in particular to a method for the propagation of arbuscular mycorrhizal fungi. Background technique [0002] Arbuscular mycorrhizal fungi are widely distributed soil microorganisms. Arbuscular mycorrhizal fungi can improve the nutrient absorption of host roots and enhance the comprehensive resistance of crops. At present, there are many methods for the propagation of fungi. For arbuscular mycorrhizal fungi, ordinary soil or fine sand is used to cultivate plants such as clover, and the symbiosis of roots and mycorrhizal fungi is used to cultivate and propagate. The specific cultivation methods are as follows: [0003] 1. Sandy soil pot culture method: [0004] Most of the conventional cultures are cultivated in sandy soil pots, that is, the spores of arbuscular mycorrhizal fungi are inoculated into the pot substrate where plants such as active clover grow, and finally a mix...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G7/06
CPCA01G31/00A01G7/06A01G24/00
Inventor 贺超兴李衍素闫妍于贤昌
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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