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Application of IPCEF1 (Interaction Protein For Cytohesin Exchange Factors 1) in diagnosing and treating osteosarcoma

A technology for osteosarcoma and therapeutic drugs, applied in the field of biomedicine, can solve the problems of low incidence of osteosarcoma, few molecular markers, and no in-depth research, etc., and achieve good application prospects, fast and accurate quantification, and high sensitivity.

Inactive Publication Date: 2017-05-24
BEIJING MEDINTELL BIOMED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low incidence of osteosarcoma, about 0.3 / 10,000, it is difficult to collect samples and has not been studied in depth. Currently, there are not many molecular markers for osteosarcoma

Method used

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  • Application of IPCEF1 (Interaction Protein For Cytohesin Exchange Factors 1) in diagnosing and treating osteosarcoma
  • Application of IPCEF1 (Interaction Protein For Cytohesin Exchange Factors 1) in diagnosing and treating osteosarcoma
  • Application of IPCEF1 (Interaction Protein For Cytohesin Exchange Factors 1) in diagnosing and treating osteosarcoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 High-throughput sequencing and analysis

[0039] 10 samples of osteosarcoma cases and 5 cases of normal control tissues were collected, among which 5 were primary samples and 5 were metastatic samples in the 10 cases of osteosarcoma. Perform RNA extraction and agarose gel electrophoresis after RNA extraction. From the electrophoresis results, it can be preliminarily judged whether the quality of the extracted RNA sample is qualified or not, and whether it can be used for further transcriptome analysis. Then, the extraction of RNA samples was detected by NanoDrop1000 spectrophotometer, and the sample requirements for RNA-seq sequencing: OD260 / OD280 was 1.8-2.2.

[0040] The sequencing platform is the HiSeq 2500 high-throughput sequencing platform of Illumina, which performs high-throughput transcriptome deep sequencing. After sequencing, we use Fast-QC software to evaluate the quality of the sequencing data as a whole, including the quality value distribution o...

Embodiment 2

[0041] Example 2 Osteosarcoma group and normal group IPCEF1 gene expression

[0042] 1. Materials and methods

[0043] 1. Materials

[0044] 17 osteosarcoma tissue samples, 28 osteosarcoma blood samples, 6 normal bone tissue controls, and 20 normal blood samples were collected, grouped and numbered.

[0045] Tissue extraction: During the patient's first operation, tissue samples were extracted during the operation under aseptic operation, and each piece was cut into the size of soybeans. After taking it out, it was quickly put into a cryopreservation tube, and placed in liquid nitrogen as soon as possible.

[0046] Blood extraction: After the patient is confirmed, collect 3-5 ml of peripheral blood samples in time, put them into EDTA anticoagulant tubes quickly, and place them in a -80°C refrigerator as soon as possible.

[0047] 2. Method

[0048] 2.1 Extraction of osteosarcoma and normal control total RNA

[0049] use Reagent (invitrogen, Cat. No. 15596-018) was used t...

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Abstract

The invention relates to application of IPCEF1 (Interaction Protein For Cytohesin Exchange Factors 1) in diagnosing and treating osteosarcoma. In recent years, the treatment of the osteosarcoma encounters the bottleneck, and particularly in an aspect of early diagnosis of osteosarcoma transfer, a related molecular tumor marker is clinically in urgent need. Osteosarcoma case samples are collected for carrying out high-throughput sequencing by an inventor, genes IPCEF1 which are closely related to the osteosarcoma are picked out by adopting bioinformatics analysis, the relevance of the IPCEF1 and the osteosarcoma is verified in a large sample through a molecular biology experiment, the molecular biology experiment shows that the genes IPCEF1 are expected to be a diagnosis molecular marker of the osteosarcoma, and an ideal is provided for early diagnosis of the osteosarcoma.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of IPCEF1 in the diagnosis and treatment of osteosarcoma. Background technique [0002] Osteosarcoma is a primary malignant bone tumor originating from bone mesenchymal cells. The age of onset is mostly 8-25 years old. It is extremely harmful to society. Its hematogenous metastasis occurs early and has a high incidence and rapid progress. 80% of The patient had micrometastases at the time of diagnosis. With the development of chemotherapy, surgical techniques, bone reconstruction and other treatment methods, the 5-year overall survival rate has been greatly improved. However, in recent years, the treatment of osteosarcoma has encountered a bottleneck, especially in the early metastasis of osteosarcoma, that is, the early diagnosis of osteosarcoma has become a clinical problem to be solved urgently. [0003] The study of tumor markers makes the early diagnosis of osteo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68G01N33/577G01N33/574
Inventor 石小峰任静向常娟
Owner BEIJING MEDINTELL BIOMED CO LTD
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