Preparation method of short-peptide/silicon dioxide/hydroxyapatite porous composite material

A porous composite material, hydroxyapatite technology, which is applied in medical science, tissue regeneration, prosthesis, etc. The effect of regulating mechanical properties, facilitating adhesion proliferation, and environment-friendly preparation process

Active Publication Date: 2017-05-17
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods either require the addition of organic solvents, or require harsh conditions such as high temperature and high pressure, and the pore structure of the prepared porous material is unevenly distributed, and the control of the pore size is also relatively difficult, which makes the prepared material subject to greater clinical application. limit

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  • Preparation method of short-peptide/silicon dioxide/hydroxyapatite porous composite material
  • Preparation method of short-peptide/silicon dioxide/hydroxyapatite porous composite material
  • Preparation method of short-peptide/silicon dioxide/hydroxyapatite porous composite material

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preparation example Construction

[0029] The embodiment of the present invention provides a preparation method of short peptide / silicon dioxide / hydroxyapatite porous composite material, including:

[0030] S1: Dissolve short peptide molecules in water, ultrasonically disperse them, adjust to a specified pH value, place them at a certain temperature for assembly, and obtain a solution containing short peptide self-assembled bodies, that is, short peptide solutions.

[0031] In this step, the non-covalent interaction between amphiphilic short peptide molecules is used to construct a short peptide self-assembly with mineralization activity. Specifically, dissolve the weighed short peptide powder in ultrapure water, disperse it with ultrasound, adjust it to a specified pH value and let it stand for assembly. Before using it in the next step, ensure that it is fully assembled. You can use an atomic force microscope or The assembly was observed by transmission electron microscopy. Through this step, a short peptide...

Embodiment 1

[0049] First prepare a 4mM amphiphilic short peptide solution, and place it at room temperature until it self-assembles; add 40 μL tetraethyl orthosilicate and 1wt% polyvinyl alcohol to 1 mL of the assembled amphiphilic short peptide solution, shake evenly Then react at 25°C for 3 days to obtain a short peptide / silica gel; replace the obtained gel with ultrapure water for 6 times, then conduct freezing induction treatment with a -80°C refrigerator, lyophilize and soak in simulated body fluid After 3 days, the short peptide / silica / hydroxyapatite porous composite material 1 was obtained.

Embodiment 2

[0051] First prepare a 10mM amphiphilic short peptide solution, and place it at room temperature until it self-assembles; add 200 μL tetraethyl orthosilicate and 2wt% sodium alginate to 1 mL of the assembled amphiphilic short peptide solution, shake evenly Then react at 25°C to obtain a short peptide / silica hydrogel; replace the obtained gel with ultrapure water for 6 times, and then conduct freezing induction treatment with liquid nitrogen at -196°C, freeze-dry it and place it in simulated body fluid After soaking for 5 days, the short peptide / silicon dioxide / hydroxyapatite porous composite material 2 was obtained.

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Abstract

The invention relates to a preparation method of a short-peptide / silicon dioxide / hydroxyapatite porous composite material, belonging to the technical field of preparation of biological composite materials. The short-peptide / silicon dioxide / hydroxyapatite porous composite material can be used as a bone-repairing three-dimensional scaffold material, has good biological activity, mechanical property and degradation characteristic, and is beneficial to improvement of the bone repairing effect. The preparation method comprises the steps of dissolving short-peptide molecules into water for assembly; mixing the assembled short-peptide solution, a silicon precursor and an adhesive, and reacting to form short-peptide / silicon dioxide gel; replacing the gel by using ultra-pure water and then freezing a sample by using a condensation induction method and carrying out freeze-drying to obtain a short-peptide / silicon dioxide porous material; soaking the material by using simulated body fluid, and carrying out freeze-drying to obtain the short-peptide / silicon dioxide / hydroxyapatite porous composite material. The short-peptide / silicon dioxide / hydroxyapatite porous composite material is used for preparing an organic / inorganic porous composite material, has a hierarchical porous structure which is easy to regulate and control, and can be applied to the field of bone repairing as a three-dimensional scaffold of an artificial bone material.

Description

technical field [0001] The invention belongs to the technical field of biological composite material preparation, and in particular relates to a preparation method of a short peptide / silicon dioxide / hydroxyapatite porous composite material for repairing bone damage. Background technique [0002] my country is a large country with a population of more than 1.3 billion. Every year, the number of patients with bone injuries caused by traffic and sports accidents reaches more than one million, and diseases such as osteoporosis and bone tissue necrosis caused by the aging population are increasing day by day. Although bone tissue has a certain self-repair ability, self-repair can only be carried out in the case of small defects. The current main treatment method is to promote the regeneration and repair of bone tissue by implanting bone repair materials in the lesion area. Therefore, bone repair materials are currently one of the most in-demand biomedical materials. [0003] Acc...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/02A61L27/12A61L27/50A61L27/56A61L27/58
CPCA61L27/025A61L27/12A61L27/22A61L27/50A61L27/56A61L27/58A61L2430/02
Inventor 王生杰部勇夏永清王晓娟曹美文
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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