Method for preparing Nisin/chitosan/polylactic acid composite nanofiber mats by coaxial electrospinning

A technology of coaxial electrospinning and composite nanofibers, which is applied in fiber processing, filament/thread forming, textiles and papermaking, etc., can solve the problems of low efficiency and difficulty in greening the purification process, and achieve improved thermal stability, Improve antibacterial stability, easy processing effect

Inactive Publication Date: 2017-05-17
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In contrast, natural ingredients extracted from plants are safer and less toxic when used as natural antibacterial agents, but it should be noted that the efficiency of extracting natural products from plants is relatively low, and a large amount of organic solvents are used in the extraction process , the purification process is difficult to green

Method used

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  • Method for preparing Nisin/chitosan/polylactic acid composite nanofiber mats by coaxial electrospinning
  • Method for preparing Nisin/chitosan/polylactic acid composite nanofiber mats by coaxial electrospinning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dissolve chitosan in 90 v.% acetic acid solution and stir to dissolve to obtain a chitosan solution with a concentration of 1.5 wt.%; add Nisin and stir to obtain Nisin / Chitosan with a Nisin concentration of 1.5 wt.% Sugar solution, used as shell spinning solution for coaxial electrospinning; dissolving polylactic acid in dichloromethane to obtain a polylactic acid solution with a concentration of 10 wt.%, used as core material for coaxial electrospinning liquid; the shell spinning solution and the core spinning solution were electrospun coaxially, at a spinning voltage of 20 KV, a receiving distance of 12 cm, a flow rate of the shell spinning solution of 1.2 ml / h, and a flow rate of the core spinning solution Under the condition of 1.0 ml / h, the Nisin- / chitosan / polylactic acid composite nanofiber mat was prepared with Nisin and chitosan as the shell layer and polylactic acid as the core material. The fiber diameter was about 185 nm. The scanning electron microscope ima...

Embodiment 2

[0023] Dissolve chitosan in 70 v.% acetic acid solution and stir to dissolve to obtain a chitosan solution with a concentration of 1.2 wt.%. Add Nisin and stir to obtain Nisin / chito with a Nisin concentration of 2.4 wt.%. Sugar solution, used as shell spinning solution for coaxial electrospinning; dissolving polylactic acid in dichloromethane to obtain a polylactic acid solution with a concentration of 8 wt.%, used as core material for coaxial electrospinning liquid; the shell spinning solution and the core spinning solution were electrospun coaxially, at a spinning voltage of 8 KV, a receiving distance of 18 cm, a flow rate of the shell spinning solution of 1.5 ml / h, and a flow rate of the core spinning solution Under the condition of 1.2 ml / h, a Nisin / chitosan / polylactic acid composite nanofiber mat with Nisin and chitosan as the shell layer and polylactic acid as the core material was prepared, with a fiber diameter of about 210 nm.

[0024] Antibacterial performance test: ...

Embodiment 3

[0026]Dissolve chitosan in 80 v.% acetic acid solution and stir to dissolve to obtain a chitosan solution with a concentration of 2.0 wt.%; add Nisin and stir to obtain Nisin / chito with a Nisin concentration of 4.0 wt.% Sugar solution is used as the shell spinning solution of coaxial electrospinning; polylactic acid is dissolved in methylene chloride to obtain a concentration of 9% polylactic acid solution, which is used as the core spinning solution of coaxial electrospinning; The shell spinning solution and the core spinning solution were electrospun coaxially, at a spinning voltage of 30 KV, a receiving distance of 15 cm, a flow rate of the shell spinning solution of 0.8 ml / h, and a flow rate of the core spinning solution of 0.6 ml Nisin / chitosan / polylactic acid composite nanofiber mat with Nisin and chitosan as the shell layer and polylactic acid as the core material was prepared under the condition of / h, and the fiber diameter was about 150 nm.

[0027] Antibacterial per...

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Abstract

The invention discloses a method for preparing Nisin/chitosan/polylactic acid composite nanofiber mats by coaxial electrospinning. The method comprises the following steps: dissolving chitosan in an acetic acid solution, stirring and dissolving to prepare a chitosan solution; adding Nisin, stirring to prepare a Nisin/chitosan solution which is used as a shell spinning solution for coaxial electrospinning; dissolving polylactic acid in dichloromethane to prepare a polylactic acid solution which is used as a core spinning solution for coaxial electrospinning; and carrying out coaxial electrospinning on the shell spinning solution and the core spinning solution to prepare the Nisin/chitosan/polylactic acid composite nanofiber mat with Nisin and chitosan as the shell and with polylactic acid as the core. The method is simple and feasible, and cost is low. The prepared biodegradable bioactive antiseptic composite nanofiber mat can be used in the fields of wound dressing, tissue engineering and food package and the like, has excellent antibacterial effects, is safe and harmless, and will not cause drug resistance of bacteria.

Description

technical field [0001] The invention relates to the field of preparation of antibacterial nanofiber materials, in particular to a method for preparing Nisin / chitosan / polylactic acid composite nanofiber felt by coaxial electrospinning. Background technique [0002] Electrospinning is a very efficient and simple method to prepare ultrafine or even nanofibers. Nanofibers and nanofibrous membranes prepared by electrospinning technology have large specific surface area and porosity, making them exhibit good performance in many fields such as filtration, adsorption, nanocomposites, tissue engineering scaffolds, and wound dressings. The nanofibrous membrane prepared by electrospinning can block wound infection caused by bacteria and dust, and has excellent air permeability, so it has a wide range of applications in the field of wound dressing. Ideal wound dressings often have high standards and requirements, and have high requirements for the biocompatibility and biological acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/64A61L15/46A61L15/32A61L15/28A61L15/26D04H1/728D01D5/00
CPCA61L15/32A61L15/26A61L15/28A61L15/46A61L15/64D01D5/0015D04H1/728C08L89/00C08L5/08C08L67/04
Inventor 胡梦欣董峰峰李吉年
Owner ZHEJIANG UNIV
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