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Method for real-time detection of monoamine neurotransmitter change in brains of animals under smoke exposure condition

A neurotransmitter, real-time detection technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problem of inability to achieve simultaneous smoke inhalation exposure and monoamine neurotransmitters, and achieve short sampling intervals and high sensitivity. , the effect of fast analysis

Active Publication Date: 2017-05-10
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the inability to simultaneously realize smoke inhalation exposure and online determination of monoamine neurotransmitters, and a specially developed analysis method for real-time monitoring of changes in monoamine neurotransmitters in animal brains under conditions of smoke exposure method

Method used

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  • Method for real-time detection of monoamine neurotransmitter change in brains of animals under smoke exposure condition
  • Method for real-time detection of monoamine neurotransmitter change in brains of animals under smoke exposure condition
  • Method for real-time detection of monoamine neurotransmitter change in brains of animals under smoke exposure condition

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Experimental program
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Effect test

Embodiment 1

[0045] Detection test of the changes of monoamine neurotransmitters in the brain of rats after a reference cigarette smoke exposure

[0046] Rat brain microdialysis probe catheter implantation test was performed according to step (1) above, followed by reference cigarette smoking and rat smoke inhalation exposure according to step (2) to expose experimental rats to TPM The concentration is 200 mg / m 3 Then follow step (3) for microdialysis test, follow step (4) for sample detection, and finally follow step (5) for sample data analysis.

[0047] image 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7 Schematic diagrams of the concentration-time curves of DA, DOPAC, HVA, 5-HT and 5-HIAA in rat brain before and after exposure to cigarette smoke, respectively.

[0048] The results showed that during exposure to cigarette smoke, the release of DA and 5-HT in the nucleus accumbens of rats continued to increase, the content of DOPAC, the primary metabolite of DA, did not chan...

Embodiment 2

[0050] Detection test of monoamine neurotransmitter changes in rat brain after exposure to electronic cigarette smoke

[0051] According to the above step (1), the catheter implantation test of the microdialysis probe in the rat brain was carried out, and then according to the step (2), the aerosol generator was used to generate the electronic cigarette aerosol and the rat smoke was inhaled through the mouth and nose, so that Experimental rats were exposed to a TPM concentration of 1000 mg / m 3 Then follow step (3) for microdialysis test, follow step (4) for sample detection, and finally follow step (5) for sample data analysis.

[0052] Figure 8 , Figure 9 , Figure 10 , Figure 11 and Figure 12 Schematic diagrams of the concentration-time curves of DA, DOPAC, HVA, 5-HT and 5-HIAA in rat brains before and after exposure to e-cigarette smoke, respectively. The results showed that the changes of DA and 5-HT in the nucleus accumbens of rats during exposure to electronic ...

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Abstract

A method for real-time detection of monoamine neurotransmitter change in brains of animals under a smoke exposure condition is characterized by exposing the animals to smoke in real time by adopting an animal oro-nasal inhalation smoke exposure system, collecting an animal brain tissue sample through an on-line microdialysis technique, and quickly analyzing monoamine neurotransmitters and metabolite thereof in a brain dialysate sample by using a high performance liquid chromatography / coularray detector, so as to detect monoamine neurotransmitter change characteristics in the brains of the animals before and after smoke exposure in real time. According to the method provided by the invention, exposure to the smoke of the animals is more similar to the manner of human beings smoking tobacco products, the technical difficulty of incapability of real-time simulating and evaluating of monoamine neurotransmitter change caused by smoke inhaling (nicotine intaking) is overcome, the method has the advantages of simple operation, accurate and reliable result and the like, and compared with the prior art, a new method for monitoring monoamine neurotransmitter metabolic change in the brains of the animals after smoke exposure is created.

Description

technical field [0001] The invention relates to a method for real-time detection and analysis of changes in monoamine neurotransmitters in animal brains under conditions of smoke exposure. The method uses online microdialysis technology to collect experimental animal brain tissue samples exposed to smoke in real time, and then uses high-performance liquid phase The chromatographic-coulometric array electrochemical detector was used to conduct rapid online analysis of monoamine neurotransmitters and their metabolites in brain dialysate samples, so as to evaluate the change characteristics of monoamine neurotransmitters before and after smoke inhalation exposure in real time. Background technique [0002] Both cigarette smoke and e-cigarette aerosol contain an important characteristic component of tobacco - nicotine (Nicotine). The occurrence of nicotine dependence is the main cause of smoking addiction, and it is also the root cause of continuous smoking of tobacco products t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/042
Inventor 毛健刘俊辉张文娟张其东李鹏柴国璧张建勋孙世豪宗永立张启东曾世通席辉
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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