Culture medium additive for preparing autophagosomes-type tumor vaccine and preparing method thereof

Abstract

The invention discloses a culture medium additive for preparing autophagosome-type tumor vaccine. A freeze-dried powder preparation comprises one group of compositions, and the compositions comprise a proteasome inhibitor, an autophagy inducer, a lysosomal alkalifier, an excipient, and an antioxidant. The culture medium additive for preparing autophagy small-body-type tumor vaccine and a preparing method thereof have the remarkable advantages that the additive compositions comprise the proteasome inhibitor, the lysosomal alkalifier and the autophagy inducer which synthesize with one another, on one hand, a proteasome degradation pathway and an autolysosome degradation pathway of a tumour cell can be inhibited simultaneously so that tumour antigens can be preserved; on the other hand, autophagosomes are induced to take shape, thus a large quantity of tumour antigens are preserved in the autophagosomes, and then the autophagosomes-type tumor vaccine can be prepared by extracting the autophagosomes.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a cell culture medium additive freeze-dried powder for preparing autophagy small subcellular tumor vaccines and a preparation method thereof. Background technique [0002] The proteins produced by the metabolism of tumor cells can be divided into two categories: long-lived proteins and short-lived proteins. The former has a long average half-life and stable structure, and is mainly degraded through the autophagy-lysosome pathway; the latter has a short average half-life and is mainly degraded by ubiquitin- Degradation by the proteasomal pathway. Studies have shown that more than 80% of the antigenic polypeptides directly presented by tumor cells are derived from short-lived proteins, and short-lived proteins are difficult to preserve due to their extremely short half-life. Proteasome inhibitors are applied to tumor cells to block the degradation of tumor short-lived proteins an...

AI Technical Summary

Problems solved by technology

Considering that mTOR, the target of rapamycin, has many complex and important functions in organisms, such as regulating ribosome generation and protein translation, it may not be conducive to the secretion of certain tumor antigens in cells while inducing autophagy in tumor cells. Synthesis will eventually affect the quality of autophagy small subcellular tumor vaccines, coupled with the adverse effects of rapamycin on the body and immunosuppression, so it is necessary to develop a new type of vaccine that is not affected by rapamycin and is specially used for the preparation of autophagy Cell culture medium additive composition for small body subcellular tumor vaccine

At present, there is no public report on the lyophilized powder preparation of the cell culture medium additive composition specially used for the preparation of the autophagy small subcellular tumor vaccine

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