Chondroitin sulfate extraction method

Abstract

The invention relates to a chondroitin sulfate extraction method, which comprises: crushing animal cartilage, placing into a reaction tank, adding demineralized water, adjusting the pH value of the solution by using an acid or alkali, adding the mixture of neutral protease or papaya enzyme and sodium borohydride or potassium borohydride, and carrying out temperature control stirring enzymolysis extraction; adding an acid or alkali to the extraction liquid, adjusting the pH value of the solution, heating the material to a temperature of 80 DEG C, and filtering after heat treatment; adding a composite protein precipitation inhibitor to the filtrate, adding ethanol to achieve an ethanol concentration of 70%, precipitating chondroitin sulfate, carrying out pressure filtration, and separating to obtain a chondroitin sulfate precipitate crude product and a protection filtrate, wherein the use amount of the composite protein precipitation inhibitor is 0.1-0.6% of the mass of the cartilage; adding demineralized water to the chondroitin sulfate precipitate, dissolving, using the composite protein precipitation inhibitor having the amount of 0.01-0.1% of the mass of the cartilage, precipitating the chondroitin sulfate by using ethanol, carrying out pressure filtration to obtain a chondroitin sulfate precipitate, dewatering the precipitate, and carrying out vacuum drying at a temperature of 50-60 DEG C to obtain a chondroitin sulfate product; and feeding the protein filtrate into a protection separation and drying tower, carrying out low temperature recovery on the ethanol, and carrying out spray drying to prepare the protein powder.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of biological extraction, and relates to a method for preparing chondroitin sulfate and protein powder by adopting technological steps such as enzymatic hydrolysis extraction, precipitation separation, dissolution and refining, product dehydration and drying, especially a method for extracting chondroitin sulfate. [0003] technical background: [0004] Chondroitin Sulfate is a natural mucopolysaccharide extracted from animal cartilage, mostly found in the cartilage of pigs, cattle, sheep, chickens and sharks. Natural mucopolysaccharides are mainly used to treat cardiovascular and cerebrovascular diseases and joint diseases, and have the functions of lowering blood fat, antithrombotic, and antitumor. Medicines and nutraceuticals rich in chondroitin sulfate have been deeply developed abroad, and chondroitin sulfate has good tolerance and safety, and will be further developed and applied. my country's ...

AI Technical Summary

Problems solved by technology

[0005] At present, there are nearly a hundred factories producing chondroitin sulfate in China. There are three main production methods: salt hydrolysis, alkali hydrolysis and enzymatic hydrolysis. The method used more in China is the combination of dilute alkali or concentrated alkali and enzymatic hydrolysis. The traditional process of extracting chondroitin sulfate is: cartilage cooking → alkali extraction → enzymatic hydrolysis → hydrogen peroxide decolorization → alcohol precipitation → refining → finished product of chondroitin sulfate. After 3-5 days, the product quality and yield were unstable. During the process of high-temperature cooking, alkali extraction, enzymolysis and decolorization, chondroitin sulfate was severely damaged, the product yield was low, the color of the solution deepened, the consumption of ethanol was large, and the production high cost;

[0006] There are chondroitin sulfate and a large amount of protein in animal cartilage. However, a large amount of animal protein and its degradation products produced in the production process of chondroitin sulfate are discharged without utilization. The untreated organic wastewater is black and smelly, and is eutrophic. Serious pollution of the water environment

At present, only a small number of relatively large-scale chondroitin sulfate production enterprises treat sewage, and generally adopt simple physical and chemical treatment as the main terminal treatment method, that is, a large amount of protein pollutants are all concentrated in the tail treatment, and the one-time investment is relatively large, and the operation cost High, the protein has not been utilized, and it is difficult to meet the discharge standard

Although some processes also delete the alkaline hydrolysis process and directly extract chondroitin sulfate by enzymatic hydrolysis, the end point of enzymatic hydrolysis is difficult to control. After protein hydrolysis, the solution becomes viscous, and it is very difficult to filter. At the same time, the adsorption of a certain amount of chondroitin sulfate affects the yield. , and the protein has not been utilized, still causing pollution to the water environment

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