Preparation method of poly hydroxybutyrate caproic-acid copolyester tissue engineering scaffold

A technology of polyhydroxybutyric acid caproic acid copolyester and tissue engineering scaffold, which is applied in the field of biomedical materials and can solve the problems of destroying the biological activity of cells and tissues, cytotoxicity, affecting the formation and repair effect of new tissues, etc.

Inactive Publication Date: 2017-05-10
王淑芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvents and cross-linking agents used in the prior art are difficult to remove, resulting in cytotoxicity when tissue engineering scaffold materials are used, causing inflammatory reactions in surrounding tissues, destroying the biological activity of cells and tissues, and affecting the formation of new tissues and repair effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of polyhydroxybutyrate caproic acid copolyester tissue engineering scaffold is realized through the following process:

[0026] (1) Prepare polyhydroxybutyrate caproic acid copolyester (granule), polyethylene oxide (powder), sodium bicarbonate (powder) according to the volume ratio of 1:1:1 to prepare a mixed solution;

[0027] (2) spinning the raw material mixture in step (1) to obtain polyhydroxybutyrate caproic acid copolyester nanofibers;

[0028] (3) Put the polyhydroxybutyrate caproic acid copolyester nanofiber of step (2) into the tissue engineering scaffold mold, then put the tissue engineering scaffold mold with the nanofiber into the supercritical CO with a pressure of 20MPa 2 Medium treatment, control the treatment temperature at 55°C, treat for 1 hour, and then depressurize and discharge CO 2 , the control time is 1 minute, the temperature during decompression is controlled at 70 ℃, and the intermediate product is obtained. The obtained...

Embodiment 2

[0030] Prepare polyhydroxybutyrate caproic acid copolyester (granular), polyethylene oxide (powder), sodium bicarbonate (powder) according to the volume ratio of 3:2:3, the same steps as in Example 1, parameters adjustment, where the supercritical CO 2 The middle treatment temperature is controlled at 55°C, treated for 1 hour, and then decompressed to discharge CO 2 , the control time is 1 minute, and the temperature during decompression is controlled at 70° C. to prepare a porous material polyhydroxybutyrate caproic acid copolyester tissue engineering scaffold interconnected inside. The porosity of the obtained porous material is 73.8%. The size of the cells is 20 microns to 250 microns, and the size of the internal interconnected channels is 20 microns to 200 microns.

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Abstract

The invention relates to a preparation method of a poly hydroxybutyrate caproic-acid copolyester tissue engineering scaffold. The preparation method comprises the steps that after 25-40% of poly hydroxybutyrate caproic-acid copolyester, 30-45% of polyoxyethylene and 25-40% of sodium bicarbonate are prepared into a mixed solution, spinning is performed to obtain poly hydroxybutyrate caproic-acid copolyester nanofiber, the nanofiber is processed in supercritical CO2, an intermediate product is prepared by controlling the processing pressure and processing temperature, the obtained intermediate product is drained and filtered in a circulating water bath and then is subjected to vacuum drying, and the tissue engineering scaffold with the porosity up to 68-74.0%, the bubble hole size of 20-200 microns and the internal intercommunication channel size of about 20-200 microns and made from a porous material can be obtained. The preparation method can complete fiber crosslinking and fusion between in a low temperature state without solvents, crosslinking agents and the like, and cytotoxicity is not produced.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a preparation method of polyhydroxybutyrate caproic acid copolyester tissue engineering scaffold. Background technique [0002] Tissue engineering scaffold material is a material that can be combined with tissue living cells and can be implanted in organisms. It can provide cells with a place to obtain nutrients, gas exchange, excrete waste, and grow and develop. The material basis of organs. [0003] For tissue engineering scaffolds, tissue engineering scaffold materials are the key to the regeneration of tissue defects. The porosity and pore size of tissue engineering scaffold materials are important factors affecting the performance of tissue engineering scaffold materials. In addition to requiring tissue engineering scaffold materials to have a high porosity, there are also strict requirements on the size of the pores. If the pores are too small, ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/50A61L27/18
CPCA61L27/56A61L27/18A61L27/50
Inventor 王淑芳
Owner 王淑芳
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