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Application of verbascoside in preparation of medicine for treating oral squamous cell carcinoma

A technology of verbasin and oral squamous cell carcinoma, applied in the field of medicine

Inactive Publication Date: 2017-05-10
AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Verbacoside has a wide range of pharmacological activities such as anti-cancer (such as: liver cancer, gastric cancer), anti-inflammatory, anti-bacterial, anti-oxidation, immune regulation, etc., but so far there is no report on its treatment of oral squamous cell carcinoma

Method used

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  • Application of verbascoside in preparation of medicine for treating oral squamous cell carcinoma
  • Application of verbascoside in preparation of medicine for treating oral squamous cell carcinoma
  • Application of verbascoside in preparation of medicine for treating oral squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The effect of verbascoside on the proliferation and apoptosis of HN4 cells.

[0031] Experimental purpose: To observe the effect of verbascoside on the proliferation and apoptosis of HN4 cells.

[0032] Experimental cells: HN4 oral squamous cell carcinoma cells, purchased from the Institute of Cells, Shanghai Academy of Biological Sciences.

[0033] Experimental drugs: verbascoside was purchased from the Northwest Institute of Plateau Biology, Chinese Academy of Sciences, dissolved in sterile PBS (phosphate buffer saline), and the negative control was PBS.

[0034] Experimental method: Using MTT colorimetry and AO / EB staining techniques, it was shown that verbascoside can effectively inhibit the proliferation of HN4 cells and induce apoptosis of HN4 cells. Specific steps are as follows:

[0035] 1. MTT colorimetric method to detect cell proliferation

[0036] 1) Cell inoculation: HN4 cells were prepared into a single cell suspension with high-sugar DMEM / F-1...

Embodiment 2

[0064] Example 2 Effect of verbascoside on tumor growth in tumor-bearing nude mice.

[0065] The nude mouse model with subcutaneous tumor implantation was used to detect the effect of verbascoside on the growth of HN4 squamous cell carcinoma tumors, and the specific steps were as follows:

[0066] 1. Animal experiments

[0067] Healthy 3-4 week old female BALB / c nude mice were purchased from the Model Animal Institute of Nanjing University, and those with no adverse reactions and normal diet, drinking water and activities were included in the experiment after feeding for 3 days. The collected HN4 cells were resuspended in serum-free high-glucose DMEM / F-12 and counted (approximately 1×10 7 pieces / ml). Take 100μl (about 10 6 cells) were injected subcutaneously in the anterior right armpit of mice, and after 7-10 days, the tumors were uniform in size (volume about 300 mm) 3 ) tumor-planted mice were randomly divided into 2 groups, 10 in each group. Physiological saline (PBS)...

Embodiment 3

[0095] Example 3 The effect of verbascoside on the main organs of tumor-bearing nude mice.

[0096] Using the nude mouse model of subcutaneous tumor implantation in Example 2 to detect the effect of verbascoside on the main organs of tumor-bearing nude mice, the specific steps are:

[0097] 1. Drug administration experiment

[0098] Prepare tumor-bearing nude mice according to the method of the animal experiment in Example 2, and the tumor size is uniform (volume is about 300mm) 3 ) tumor-planted mice were randomly divided into 2 groups, 10 in each group. Physiological saline (PBS) and verbascoside were injected intraperitoneally into the tumor-bearing mice every day for 2 weeks. During this period, the survival of the tumor-bearing mice was monitored. If there was death, it should be dissected immediately to find the cause of death. After 2 weeks of administration, the mice were sacrificed, and the heart, liver and kidney tissues of the mice in each group were taken out and...

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Abstract

The invention provides an application of verbascoside in preparation of a medicine for treating oral squamous cell carcinoma. By intraperitoneal injection of verbascoside into a tumor-bearing mouse with oral squamous cell carcinoma, it is observed that the verbascoside can effectively inhibit the growth of oral squamous cell carcinoma tumors by promoting tumor cell apoptosis. Moreover, the verbascoside provided by the invention, at cell and animal levels, achieves an effect of effectively resisting squamous cell carcinoma by inhibiting proliferation of HN4 cells of the oral squamous cell carcinoma and inducing apoptosis of the HN4 cells.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the use of verbascoside in the preparation of medicines for treating oral squamous cell carcinoma. Background technique [0002] Oral squamous cell carcinoma (OSCC), referred to as oral squamous cell carcinoma, is one of the most common malignant tumors of the oral and maxillofacial region, with a high incidence rate and a tendency of early extensive lymph node metastasis. At present, surgery, radiotherapy and chemotherapy are still important methods for the treatment of oral squamous cell carcinoma clinically, but these methods all have defects. For example, the application of surgery is limited due to the incompleteness of local treatment and the high recurrence rate after surgery; although radiotherapy and chemotherapy can effectively kill cancer cells, they can also cause damage to normal cells at the same time, leading to various complications in patients , causing great harm to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7032A61P35/00
CPCA61K9/0019A61K31/7032
Inventor 袁毅张亚琴吴金道杨建荣武和明刘宾赵晓辉王晶晶汪瀚
Owner AFFILIATED STOMATOLOGICAL HOSPITAL OF NANJING MEDICAL UNIV
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