Cultivation method for stevia rebaudiana tetraploid

A cultivation method and technology of stevia, which is applied in the field of stevia tetraploid cultivation, can solve the problems of low productivity of stevia and the inability of conventional growth to meet market demand, and achieve the effects of environmental friendliness, increased yield and broad application prospects

Inactive Publication Date: 2017-05-10
MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the technical problems of low Stevia rebaudiana productivity and conventional growth that cannot meet the market demand in the prior art, the present invention provides a method for cultivating stevia tetraploids. Stevia tetraploid plants bred by the method are due to chromosome Doubling, its medicinal ingredients increase significantly, the plant grows robustly, and has a broad application prospect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The present embodiment relates to a method for cultivating stevia tetraploids, the method for cultivating comprises the following steps:

[0026] (1) Material selection: select the seeds of Stevia rebaudiana plants that grow robustly and grow well;

[0027] (2) Seed treatment: Soak stevia seeds in detergent for 15 minutes, clean them, place them in alcohol with a volume fraction of 75%, sterilize them under ultrasonic conditions for 15 seconds, and finally wash them with sterile water for 3 times; After cleaning and disinfection, cut the seeds in a sterile culture dish, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+NAA 0.2mg / L On the culture medium, under the condition of 25~30°C, culture in shading for 10 days, switch to light culture for 28 days for sterile culture, so that the germination of the seed embryo becomes a green seed embryo protocorm;

[0028] (...

Embodiment 2

[0032] The present invention relates to a kind of cultivation method of stevia tetraploid, and described cultivation method comprises the following steps:

[0033] (1) Material selection: select the seeds of Stevia rebaudiana plants that grow robustly and grow well;

[0034] (2) Seed treatment: Soak stevia seeds in detergent for 15 minutes, clean them, place them in alcohol with a volume fraction of 75%, sterilize them under ultrasonic conditions for 20 seconds, and finally wash them with sterile water for 5 times; After cleaning and disinfection, cut the seeds in a sterile culture dish, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+NAA 0.2mg / L On the culture medium, under the condition of 25°C, culture in shading for 7 days, then switch to light culture for 30 days for sterile culture, so that the seed embryos germinate into green seed embryo protocorms;

[0035] (...

Embodiment 3

[0039] The present invention relates to a kind of cultivation method of stevia tetraploid, and described cultivation method comprises the following steps:

[0040] (1) Material selection: select the seeds of Stevia rebaudiana plants that grow robustly and grow well;

[0041](2) Seed treatment: Soak stevia seeds in detergent for 20 minutes, clean them, place them in alcohol with a volume fraction of 75%, sterilize them under ultrasonic conditions for 10 seconds, and finally wash them with sterile water for 5 times; After cleaning and disinfection, cut the seeds in a sterile culture dish, disperse the seed embryos in a small amount of sterile water, use a straw to absorb the sterile water containing the seed embryos, and inoculate them into B5+NAA 0.2mg / L On the culture medium, under the condition of 30°C, culture in shading for 10 days, then switch to light culture for 25 days for sterile culture, so that the seed embryos germinate into green seed embryo protocorms;

[0042] (...

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Abstract

The invention provides a cultivation method for a stevia rebaudiana tetraploid. The cultivation method comprises the following steps: seed selection; seed treatment: a step of subjecting stevia rebaudiana seeds to cleaning and disinfection, then cutting the stevia rebaudiana seeds open in an aseptic culture medium vessel, taking embryos out of the aseptic culture medium vessel, carrying out aseptic culturing, and allowing the embryos to germinate into green embryo protocorms; and inducing culturing: a step of inoculating protocorms on a budding inducing culture medium added with dimethyl sulfoxide and colchicine, carrying out first inducing culturing, then carrying out transferring to a culture medium only containing colchicine, carrying out secondary inducing culturing, then carrying out transferring to a culture medium without dimethyl sulfoxide and colchicine, and carrying out culturing so as to obtain a stevia rebaudiana tetraploid plant. With the cultivation method provided by the invention, the cultivated stevia rebaudiana tetraploid plant has doubled chromosome, so stevia rebaudiana has high medicinal ingredients; the yield of stevioside can be greatly improved; meanwhile, the cultivation method has the advantages of simplicity, stability, environmental friendliness, applicability to industrial production, and extensive application prospects.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for cultivating stevia tetraploids. Background technique [0002] Stevia is a perennial herbaceous plant of Compositae, native to subtropical South America. The roots, stems and leaves of Stevia rebaudiana contain steviol glycosides. Stevioside has the characteristics of high sweetness (200-300 times that of sucrose), low calorie (only 1 / 300 of sucrose), and can increase blood sugar, lower blood pressure, and promote metabolism. Heart disease, dental caries, etc. have a certain auxiliary effect, so it has increasingly attracted people's attention and attention, and has become the "third sugar source" after sucrose and synthetic sugar. [0003] Polyploid breeding is an important means of cultivating new plant varieties. Polyploid breeding originated in the early 20th century and has been widely used in the cultivation of new plant varieties. Due to the increase ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/08
CPCA01H1/08A01H4/001A01H4/008
Inventor 贾德军
Owner MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP
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