Kit for detecting MyD88 gene expression of odontobutis potamophila based on fluorescence RT-RCR and applications of kit

Abstract

The invention discloses a kit for detecting MyD88 gene expression of odontobutis potamophila based on fluorescence RT-RCR and applications of the kit. According to the kit and the applications of the kit, due to the establishment of the odontobutis potamophila MyD88 gene real-time fluorescence RT-PCR detection method, a theoretical basis is laid for researching the evolutionary status of the MyD88 gene in the immune system of odontobutis potamophila and the relevance of the MyD88 gene with disease resistance, further researching the biological activity, functions and expression regulation of the MyD88 gene and disclosing the immune anti-infection mechanism of odontobutis potamophila. MyD88 mediates the most of TLR signal transduction, and plays the important role in the inherent immune reaction, the expressive abundance of the MyD88 gene reflects the immune system activity of odontobutis potamophila to a certain degree, and can be used for judging that whether fish bodies are infected with diseases or not before the fish bodies show disease features as the important detection index for preventing and treating the diseases of odontobutis potamophila, so that effective preventing measures can be taken in advance, and the great financial loss caused by diseases can be prevented.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a fluorescent RT-RCR detection kit for detecting the expression of the MyD88 gene of the river sand pond snakehead and the application thereof. Background technique [0002] Fish resist the invasion of foreign pathogens through specific and non-specific immune systems. The body's innate immunity relies on limited pattern recognition receptors to recognize pathogen-related molecular patterns. Currently identified pattern recognition receptors mainly fall into two categories: cytoplasmic pattern recognition receptors (Cytosolic PRRs) and membrane-bound Toll-like receptors (Membrane-bound Toll-like receptors, TLRs). [0003] Toll-like receptors are the most studied pattern recognition receptors, among which MyD88 mediates most TLR signal transduction (except TLR3), is one of the most important adapter molecules, and plays an important role in innate immune resp...

AI Technical Summary

Problems solved by technology

This fish is a freshwater benthic small carnivorous fish with tender meat. In 2011, it was used as a fish for VIPs at the state banquet of the Shanghai World Expo. It is known as the "No. 1 fish in the World Expo". The price has reached more than 160 yuan / kg, which has high development value, but bacterial and fungal diseases are prone to occur in artificial breeding, resulting in serious economic losses

At present, the researches on Channa nama are mainly focused on breeding and breeding, and there are no reports on the immune-related genes and disease resistance factors.

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