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Method for preparing polypeptide and astaxanthin from shrimp and crab processing waste

A technology of astaxanthin and waste, applied in the field of preparation of polypeptide and astaxanthin, to achieve the effect of reducing environmental pollution, mild conditions and good safety

Inactive Publication Date: 2017-04-26
深圳市江牧实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the technical defects of the existing alkaline extraction and solvent extraction, and provide a method for simultaneously preparing polypeptide and astaxanthin from shrimp and crab processing waste. The method also has high raw material utilization rate and mild production process conditions Controllable, energy saving and environmental protection, good product quality, etc.

Method used

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  • Method for preparing polypeptide and astaxanthin from shrimp and crab processing waste

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Embodiment 1

[0047] A method for preparing polypeptide and astaxanthin from shrimp and crab processing waste, comprising the steps of:

[0048] 1) Raw material pretreatment:

[0049] Collect 10kg of shrimp shells, remove moldy shrimp shells, wash with tap water to remove sediment and seaweed, drain, crush and beat the slurry, pass the slurry through a 60-mesh sieve, and add a mixed solution of electrogenerated functional water and hydrochloric acid to make the pH of the system 1. After stirring at 150r / min for 6h, centrifuge at 3000r / min with a flat-panel centrifuge equipped with a 600-mesh filter cloth, collect the precipitate a, and store it at low temperature for later use.

[0050] 2) Material enzymatic hydrolysis:

[0051] Add the precipitate a to the electro-generated functional water with a pH of 8.5-9.0 at a material-to-liquid ratio of 1:10 (m / V), then add the complex protease at 3% of the enzyme amount, and stir at 300r / min at 40°C Hydrolysis 4h. After the enzymatic hydrolysis ...

Embodiment 2

[0057] A method for preparing polypeptide and astaxanthin from shrimp and crab processing waste, comprising the steps of:

[0058] 1) Raw material pretreatment:

[0059] Collect 20kg of crab shells, remove the moldy part, wash with tap water to remove sediment and seaweed, drain, crush and beat the slurry, pass the slurry through an 80-mesh sieve, add a mixed solution of electrogenerated functional water and hydrochloric acid, and make the pH of the system 0. After stirring at 200r / min for 6h, centrifuge at 3500r / min with a flat-panel centrifuge equipped with a 600-mesh filter cloth, collect the precipitate a, and store it at low temperature for later use.

[0060] 2) Material enzymatic hydrolysis:

[0061] Add the precipitate a to the electro-generated functional water with a pH of 9.0-10.0 at a material-to-liquid ratio of 1:15 (m / V), then add the complex protease at 5% of the enzyme amount, and stir at 50°C at a speed of 300r / min Hydrolysis 3h. After the enzymatic hydroly...

Embodiment 3

[0067] A method for preparing polypeptide and astaxanthin from shrimp and crab processing waste, comprising the steps of:

[0068] 1) Raw material pretreatment:

[0069] Collect 15 kg of shrimp shells, remove moldy shrimp shells, wash with tap water to remove sediment and seaweed, drain, crush and beat the slurry, pass the slurry through a 100-mesh sieve, and add a mixed solution of electrogenerated functional water and hydrochloric acid to make the pH of the system 2, After stirring at 200r / min for 9h, centrifuge at 3500r / min with a plate centrifuge equipped with an 800-mesh filter cloth, collect the precipitate a, and store it at low temperature for later use.

[0070] 2) Material enzymatic hydrolysis:

[0071] Add the precipitate a to the electro-generated functional water with a pH of 8.5-9.5 at a material-to-liquid ratio of 1:12 (m / V), then add the compound protease at 5% of the enzyme amount, and stir at 50°C at a speed of 300r / min Hydrolysis 6h. After the enzymatic h...

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Abstract

The invention belongs to the field of comprehensive utilization of subsidiary agricultural products, and particularly relates to a method for preparing polypeptide and astaxanthin from shrimp and crab processing waste. The method particularly comprises the steps that the shrimp and crab processing waste serves as a raw material, cleaning and crushing are conducted, acid soaking is conducted, after enzymatic hydrolysis and centrifugation are conducted, supernatant is separated and concentrated through a membrane, and polypeptide is obtained; centrifugal sediments are dried, and astaxanthin is obtained after subcritical extraction and column chromatography isolation. Compared with traditional alkali extraction, the method has the advantages of being high in raw material utilization rate, energy-saving and environmentally-friendly in production process and high in product purity and additional value. By means of the method, comprehensive utilization of the shrimp and crab resource is promoted, the sources of the astaxanthin and polypeptide are enriched, and the positive economic value is achieved.

Description

technical field [0001] The invention belongs to the technical field of comprehensive processing of agricultural products, and specifically relates to a method for preparing polypeptide and astaxanthin from shrimp and crab processing waste. The method prepares polypeptide and astaxanthin through processes such as enzymatic hydrolysis, subcritical extraction, and membrane separation. . Background technique [0002] China is a large aquatic country, rich in crustaceans such as shrimps and crabs. Taking shrimps as an example, China's total annual output of shrimps is about 2.5 million tons, accounting for 37% of the world's total output, ranking first in the world. In recent years, with the vigorous development of the shrimp and crab processing industry, while bringing substantial benefits to the practitioners, about 40-50% of the processing waste is produced. Due to the backwardness of the domestic processing technology, the utilization rate of these processing wastes is low. E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07C403/24
CPCC07C403/24C07K1/34C12P21/06
Inventor 王俏松芦鑫徐丽
Owner 深圳市江牧实业有限公司
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