Mouse cell L615-based HIV-1 infectible host cell
A technology for host cells and HIV-1, applied in the field of HIV-1 infectable host cells and their preparation, and can solve the problems of complete replication of difficult-to-maintain viruses and the like
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[0028] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.
[0029] 1. Preparation of HIV-1 infectable host cells
[0030] 1) Construction of lentiviral expression vector
[0031] The target genes were hCD4 (GenBank sequence number: NM_000616.4), hCCR5 (GenBank sequence number: NM_000579.3) and human Cyclin T1 (GenBank sequence number: NM_001240.3) to construct a double-promoter lentiviral overexpression vector, from The international standard gene bank was retrieved, and the expression method of bicistronic vector (Bicistronic) was used to prepare the lentiviral packaging vector. The schematic diagram of the structure of the lentiviral vector is shown in figure 1 .
[0032] Using Gateway technology, first construct pDown-CD4-T2A-Cyclin T1, pUP-CMV, pTail-IRES / CCR5; small amount of extraction plasmid pDown-...
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