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Chanterelle planting method

A cultivation method, chanterelle technology, applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems of depletion of wild resources and high price, and achieve the effect of fast growth, small investment and quick results

Inactive Publication Date: 2017-04-26
吴明金
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to the destruction of the ecological environment and the high price of wild chanterelles on the Hainan market, over-harvesting has exacerbated the depletion of this wild resource.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0055] Chanterelles were purchased from Guangdong Institute of Microbiology.

[0056] Inoculate the mycelium of chanterelles on the mother seed medium and expand the culture for 14 days to obtain the mother seed of chanterelles, inoculate the mother seed on the original seed medium and cultivate it for 20 days to obtain the original seed, and connect the original seed to the section wood for section wood cultivation Fruiting culture; common PDA medium for mother seed medium: 220 grams of potato, 22 grams of glucose, 17-22 grams of agar, 1200 ml of water; original seed medium: 82% of broad-leaved wood chips, 20% of wheat bran, 3% of sucrose , 3% gypsum powder, add water after stirring evenly, and the final water content of the medium reaches 57-62%; the original seed medium is made into a 500mL original seed bottle;

[0057] The cultivation conditions of the mother species and the original species are 24°C constant temperature darkroom cultivation; the cultivation of the mother...

Embodiment 2

[0068] The strains of chanterelles are isolated from the fruiting body of wild chanterelles, and the strains are separated by tissue separation method, that is, the fruiting bodies of wild chanterelles are cut in half with a sterile scalpel, and small squares of about 0.5 cm are cut with a knife Tissue: use tweezers to place the fungus meat tissue in the center of the PDA culture medium in the petri dish. After 4 to 5 days of culture, white hyphae can be seen growing from the tissue block, and the purified chanterelle mycelia can be obtained by transferring the tube 3 to 4 times.

[0069] Inoculate the mycelium of chanterelles on the mother seed medium and expand the culture for 15 days to obtain the mother seed of chanterelles, inoculate the mother seed on the original seed medium and cultivate it for 17 days to obtain the original seed, and inoculate the original seed on the substitute material for cultivation Fruiting culture; common PDA medium for mother seed medium;

[00...

Embodiment 3

[0090] The strain of chanterelle Ganoderma gibbosum (Nees) Pat is isolated from the fruiting body of wild chanterelle, and the strain is separated by tissue separation method, that is, the fruiting body of wild chanterelle is cut in half with a sterile scalpel, and cut with a knife Use tweezers to place the tissue in a small square of about 0.5 cm in the center of the PDA culture medium in a petri dish. After 3 days of cultivation, white hyphae can be seen growing from the tissue block. Transfer the tube 1 or 2 times to obtain purified chanterelles Silk.

[0091] Inoculate the chanterelle mycelium on the mother seed medium and expand the culture for 12 days to obtain the chanterelle mother seed, inoculate the mother seed on the original seed medium and cultivate it for 27 days to obtain the original seed, and inoculate the original seed on the section wood for section wood cultivation fruiting culture;

[0092] The yeast extract medium screened out for the mother seed medium:...

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Abstract

The invention discloses a chanterelle planting method, chanterelle hypha is planted in a mother fungi culture medium for enlarge cultivation, so that chanterelle mother fungi is obtained. The mother fungi is planted in a stock culture medium, so that the stock is obtained. The stock is grafted to a cut log for cut log cultivation or to a substitute for substitute cultivation for further fruiting cultivation. For 1000 kg log, 44 to 69 kg sporocarp can be harvested in the current year and 27 to 35 kg sporocarp can be harvested in the next year generally. For 1000 kg substitute, 52 to 74 kg sporocarp can be harvested in the current year generally. The method provided by the invention is easy and simple to apply, low in investment, quick in effect realization, easy to promote and avoids shortcomings of wild resources. By adopting the method, hypha growth speed is high and the daily growth speed of hypha can reach 16 mm / day, and the sporocarp biological science can reach 6.9 % to 12 %. The sporocarp is high in yield and good in quality.

Description

technical field [0001] The invention relates to a method for artificially cultivating medicinal fungi, in particular to a method for artificially cultivating chanterelles. Background technique [0002] Chanterelles, distributed in Jiangsu, Zhejiang, Hainan, Fujian, Guangxi and other places, are mainly distributed in Hainan, where they are used to treat stomach problems among the people in Hainan. Other effects are similar to those of tree tongue. Foreign countries are mainly distributed in Indonesia. In recent years, due to the destruction of the ecological environment and the high price of wild chanterelles on the Hainan market, over-harvesting has exacerbated the depletion of this wild resource. [0003] Morphological description of chanterelles: fungal tube dark brown, 0.7-1.2 cm long; pore surface dirty white or brown; tube mouth nearly round, 6-7 per mm. The stipe is short and thick, lateral, 6-10cm long, 3-5cm thick, thicker at the base, and the same color as the cap...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00C05G1/06
CPCA01G18/00C05B7/00C05D3/00C05G3/00C05G5/23C05C11/00C05D9/02C05C9/00C05D1/02C05F9/04
Inventor 吴明金
Owner 吴明金
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