Biscuits containing modified dietary fibers sourced from carrot peel pomace and preparation method of biscuits
A technology of dietary fiber and carrots, which is applied in baked food, food science, dough processing, etc., can solve the problems of weak function of harmful substances, single nutritional components, poor taste, etc., and achieve sufficient sources, strong specificity, and convenient operation Effect
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Embodiment 1
[0036] Example 1: Preparation of Dietary Fiber Extract from Carrot Dregs
[0037] The neutral protease used in the preparation method 1-4 of this example was purchased from Novozymes Bioengineering Company. Ingredient: The high-temperature-resistant α-amylase used in the preparation method 1-4 of this example was purchased from Tianjin Nuoao Technology Development Co., Ltd. It is a liquid with an enzyme activity of 25000U / mL, and it is obtained from Bacillus licheniformis α-amylase whose optimum temperature is high temperature.
[0038] The total dietary fiber content of the raw carrot peel dregs used in this example is 60.5%, of which: the insoluble dietary fiber content is 51.06%, and the soluble dietary fiber content is 9.44%.
[0039] Preparation method 1: enzymatic method
[0040] Accurately weigh 5.00g of carrot peel residue, add it to a beaker filled with 200mL pH 6.5 phosphate buffer solution, then add 2.04mL 1mmol·L -1 CaCl 2 Solution, and add 0.4g neutral proteas...
Embodiment 2
[0052] Example 2: Modification of dietary fiber extract derived from carrot peel
[0053] Modification method 1: compound enzyme treatment
[0054] Add 100 g of the dietary fiber extract derived from carrot peel dregs obtained in Preparation Method 4 of Example 1, add 3000 mL of pH 4.8 citric acid-sodium citrate buffer solution, add 0.9 g of cellulase with an enzyme activity of 10000 U / g and an enzyme activity of 600 U 8g of xylanase / g, form cellulase and xylanase enzymolysis system, 50 ℃ enzymolysis 2.5h, after completion, add 4 times the volume of 95% ethanol, stand at room temperature for 4h, filter dehydration, and then The filter residue was dried at 50°C to obtain the modified dietary fiber derived from carrot skin residue, which was stored in a dry and dark place.
[0055] Modification method 2: high static pressure treatment
[0056] Put 10 g of the dietary fiber extract derived from carrot peel dregs obtained in Preparation Method 4 of Example 1 into a polyethylene ...
Embodiment 3
[0089] Example 3: Properties of modified dietary fiber derived from carrot peel
[0090] 1. Modification of the optimized dietary fiber extract from carrot peel
[0091] Accurately weigh 1.00 g of the dietary fiber extract derived from carrot peel dregs obtained in Example 1, add 30 mL of pH 4.8 citric acid-sodium citrate buffer, add 0.009 g of cellulase with an enzyme activity of 10000 U / g and an enzyme activity of 600 U / g Xylanase 0.08g, placed in a shaking water bath at 50°C for 2.5h. After the enzymatic hydrolysis is completed, add 4 times the volume of 95% ethanol directly, stand at room temperature for 4 hours, filter under reduced pressure, transfer the filter residue remaining on the wall of the cup with 95% ethanol, then dry the filter residue in hot air at 50°C, and store it in a dry and dark place save. The particle size of the modified dietary fiber derived from carrot peel dregs after the above treatment is not greater than 80 mesh.
[0092] 2. Properties of di...
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