A kind of quality standard detection method of 20(r)-ginsenoside rg3
A quality inspection method and ginsenoside technology are applied in the field of quality inspection of 20-ginsenoside Rg3 raw materials, and can solve the problems that the safety and controllability of 20-(R)-ginsenoside Rg3 cannot be satisfied.
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Embodiment 1
[0321] The preparation of embodiment 1 crude drug 20 (R)-ginsenoside Rg3
[0322] Cultivated ginseng coarse powder 500g, extracted three times with hot water at 80°C, each time with 4000ml of hot water, leached for 4 hours each time; combined the extracts, filtered; concentrated the extracts to 2500ml, extracted 5 times with an equal volume of n-butanol , combine the extracts; concentrate the extracts to dryness, dissolve with 1000ml water, filter; the filtrate passes through a macroporous adsorption resin column, elutes with water, 30% ethanol, and 50% ethanol successively, and collects 50% ethanol fractions; concentrates, uses 500ml of 80% ethanol was recrystallized three times, and the resulting precipitate was dried and pulverized to obtain about 2.2g of 20(R)-ginsenoside Rg3. The raw material drug 20(R)-ginsenoside Rg3 is a white solid powder; the taste is slightly bitter.
Embodiment 2
[0323] Example 2 bulk drug 20 (R)-ginsenoside Rg3 quality standard detection
[0324] 1. Determination of 20(R)-ginsenoside Rg3 content
[0325] 1-1) Take an appropriate amount of 20(R)-ginsenoside Rg3 reference substance, grind it finely, accurately weigh 20(R)-ginsenoside Rg3 reference substance (10 mg), add methanol-water mixed solution, dissolve it ultrasonically, and prepare Ginsenoside Rg3 reference substance solution containing 0.1 mg ginsenoside Rg3 per 1 ml;
[0326] 1-2) Take an appropriate amount of the 20(R)-ginsenoside Rg3 crude drug prepared in Example 1, grind it finely and precisely weigh the ginsenoside Rg3 sample (22.1mg), place it in a 100mL volumetric flask, add methanol-water to mix Appropriate amount of solution, shake well, after dissolving, put it in an ultrasonic instrument for ultrasonic treatment for 60 minutes, then cool to room temperature (15-35°C), add methanol-water to mix and dissolve to the scale line of the volumetric flask, shake well, and ...
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