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Detection reagent for in vitro detection of content of iodine in urine

A detection reagent and in vitro detection technology, applied in the field of detection, can solve the problems of inconvenient popularization and application, long time period, long processing time, etc., achieve color gradient and color stability, high detection repeatability and accuracy , The effect of less time-consuming reaction detection

Inactive Publication Date: 2017-03-29
ANHUI HUIBANG BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, when using this method, the urine sample needs to be processed after adding ammonium persulfate and digesting the urine at a high temperature of 100°C for 60 minutes before measurement, which is dangerous and harmful, and the processing time is too long, which is not convenient for operation and time period long, and the reagents of this method, such as arsenic trioxide, which contain heavy metal compounds, are poisonous and harmful, and are easy to pollute the environment, and require special digestion and semi-automatic biochemical analyzers, which are inconvenient to be popularized and applied at the grassroots level where iodine deficiency is serious.

Method used

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  • Detection reagent for in vitro detection of content of iodine in urine
  • Detection reagent for in vitro detection of content of iodine in urine
  • Detection reagent for in vitro detection of content of iodine in urine

Examples

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Effect test

Embodiment 1

[0023] A detection reagent for in vitro detection of iodine content in urine, comprising an oxidizing agent, a color developing agent, a diluent and a urine pretreatment agent;

[0024] The oxidizing agent is composed of the following components according to the mass percentage concentration: 20% of glacial acetic acid, 15% of hydrogen peroxide, 5% of hydrochloric acid, 5% of sulfuric acid, 2.5% of quinolinic acid, and the balance of purified water. The preparation method is as follows: All raw materials, fully mixed, ready to use;

[0025] The color developing agent is a TMB alcohol solution with a mass percent concentration of 0.1%. The preparation method is as follows: take TMB, add absolute ethanol, fully stir and dissolve, then seal it, avoid light, keep away from oxidants, and store at 2-8°C;

[0026] The diluent is a citric acid-sodium citrate buffer solution with pH=4.0, and the preparation method is as follows: first prepare 0.01mol / L citric acid solution and 0.01mol / ...

Embodiment 2

[0029] A detection reagent for in vitro detection of iodine content in urine, comprising an oxidizing agent, a color developing agent, a diluent and a urine pretreatment agent;

[0030] The oxidizing agent is composed of the following components according to mass percentage concentration: 10% glacial acetic acid, 20% hydrogen peroxide, 0.1% hydrochloric acid, 10% sulfuric acid, 0.1% quinolinic acid, and the balance of purified water. The preparation method is as follows: All raw materials, fully mixed, ready to use;

[0031] The color developing agent is a TMB alcohol solution with a mass percent concentration of 0.025%. The preparation method is as follows: take TMB, add absolute ethanol, fully stir and dissolve, then seal it, avoid light, keep away from oxidants, and store at 2-8°C;

[0032] The diluent is a Tris buffer solution with pH=3.0, and the preparation method is as follows: first prepare a 0.01mol / L tris solution and a 0.01mol / L hydrochloric acid solution, and then ...

Embodiment 3

[0035] A detection reagent for in vitro detection of iodine content in urine, comprising an oxidizing agent, a color developing agent, a diluent and a urine pretreatment agent;

[0036] The oxidizing agent is composed of the following components according to mass percentage concentration: 30% of glacial acetic acid, 1% of hydrogen peroxide, 10% of hydrochloric acid, 0.1% of sulfuric acid, 5% of quinolinic acid, and the balance of purified water; the preparation method is as follows: All raw materials, fully mixed, ready to use;

[0037] The color developing agent is a TMB alcohol solution with a mass percent concentration of 0.2%. The preparation method is as follows: take TMB, add absolute ethanol, fully stir and dissolve, then seal it, avoid light, keep away from oxidants, and store at 2-8°C;

[0038] The diluent is an acetate buffer solution with pH=5.0, and the preparation method is: first prepare 0.01mol / L acetic acid solution and 0.01mol / L sodium acetate solution, then m...

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Abstract

The invention discloses a detection reagent for the in vitro detection of the content of iodine in urine. The detection reagent comprises an oxidant and a color developer; the oxidant comprises, by mass, 10-30% of glacial acetic acid, 1-20% of hydrogen peroxide, 0.1-10% of hydrochloric acid, 0.1-10% of sulfuric acid, 0.1-5% of quinaldinic acid, and the balance of purified water; and the color developer is a TMB alcohol solution with the mass percentage concentration of 0.025-0.2%. The detection reagent has the advantages of high repeatability, high accuracy, very good chromogenic gradient and chromogenic stability, simplicity in use and operation, short reaction detection time, no need of too much treatment of a urine sample, simplicity, fastness, no heavy metal compounds or aldehyde condensation products, difficulty in pollution of environment, safety, and environmental protection; and a result of quantitative detection of the content of iodine in the urine through using the detection reagent is used for evaluating the iodine nutrition condition of detected people, so the detection reagent is suitable for being promoted and applied in severe iodine deficiency base medical units.

Description

technical field [0001] The invention relates to the detection field, in particular to a detection reagent for in vitro detection of iodine content in urine. Background technique [0002] Iodine is an indispensable and important element for the synthesis of thyroid hormones and plays an important role in the development of the brain. Iodine deficiency in pregnant women can cause brain damage to fetuses and infants, resulting in irreversible mental retardation and psychomotor dysfunction, manifested as stupidity, stupidity, deaf-mute, paralysis, and poor abstract thinking ability. [0003] The detection method of existing urine iodine is urine iodine arsenic cerium catalytic spectrophotometry method, and its principle is to react yellow Ce 4+ is reduced to colorless Ce 3+ , the higher the iodine content, the faster the reaction rate, and the remaining Ce 4+ The less, the reaction temperature and time are controlled, and the remaining Ce in the system is measured at a wavele...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 王忠亮胡容吴才桂陈军超
Owner ANHUI HUIBANG BIOLOGICAL ENG
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