Hyperspectrum-based transgenic maize kernel recognition device and method

Abstract

The invention discloses a hyperspectrum-based transgenic maize kernel recognition device and method. The method comprises the following steps that crylAb / cry2Aj-G10evo transgenic maize and nonparent maize hyperspectral images are obtained through a hyperspectrum instrument; spectral information of an area of interest is obtained through an image processing method, and spectroscopic data preprocessing is performed; all spectra and extracted characteristic spectra are used for building a PLS and SVM discriminant analysis model. The recognition rate of the built transgenic maize kernel SG-PLS model is 90% or above. Compared with a traditional molecular detection method, rapid, visual and lossless identification of transgenic maize and non-transgenic maize kernels can be achieved, operation is easy, no large amount of experimental treatment is needed, the cost is low, and the good application prospect and considerable market value are achieved.

Description

technical field [0001] The invention belongs to the technical field of non-destructive detection of transgenic breeding screening, in particular to an identification device and method based on hyperspectral transgenic corn kernels. Background technique [0002] Corn (Zea mays L.) is an important food, feed and energy crop in the world, as well as an important industrial raw material, occupying an important position in the world's food production. In order to meet the continuous growth of the world population, the steady development of animal husbandry, and the large-scale promotion of bio-energy, the genetic traits of maize plants are directional modified, the linkage expression of undesirable traits is avoided, and the breeding cycle is short. Plant transgenic technology has been widely used in maize breeding Rapid development. [0003] The special growth environment of high temperature and high humidity makes cornfields extremely vulnerable to the stress of weeds and inse...

AI Technical Summary

Problems solved by technology

However, the difficulty of maize breeding is much greater than expected, the breeding efficiency is low, the offspring of hybrids often show abnormal segregation, the offspring carrying the target gene are not easy to be homozygous, and each generation must be detected and tracked

[0004] Traditional molecular detection methods will destroy proteins or gene fragments to a certain extent, damage the original appearance of genetically modified crops, time-consuming, laborious, complicated procedures, high cost, difficult for non-professionals, not suitable for real-time detection of genetically modified and non-transgenic crops Online rapid detection and identification, let alone the breeding and cultivation of genetically modified crops

At present, near-infrared spectroscopy technology has been applied in transgenic identification due to its fast and non-destructive characteristics. The technology integrates image and spectral information, and can collect the spectral information and spatial information of the research object at the same time, and can obtain a wider range of internal and external information of the sample, which can not only reflect the color, shape, size and other apparent properties of the detection object, It can also reflect the internal information such as the chemical composition and physiological structure of the test object

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