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Markers, reagent and reagent box for indicating directional differentiation potential of mesenchymal stem cells (MSCs)

A kind of stem cell, directional differentiation technology, applied in the field of stem cells, can solve the problems of long screening time, low screening efficiency, unsuitable for high-throughput screening, etc., and achieve the effect of high efficiency and high accuracy

Inactive Publication Date: 2017-03-29
南京千年健干细胞基因工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage of this method is that the screening time is long, the screening efficiency is low, and it is not suitable for high-throughput screening.

Method used

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  • Markers, reagent and reagent box for indicating directional differentiation potential of mesenchymal stem cells (MSCs)
  • Markers, reagent and reagent box for indicating directional differentiation potential of mesenchymal stem cells (MSCs)
  • Markers, reagent and reagent box for indicating directional differentiation potential of mesenchymal stem cells (MSCs)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Discovery of markers predicting the potential of bone marrow mesenchymal stem cells BMSCs to differentiate into cardiomyocytes

[0030] 1. Experimental basis (inducers: 5-azacytidine, angiotensin II, dimethyl sulfoxide)

[0031] 5-azacytidine [Li Xue et al., Experimental study on the differentiation of rat bone marrow mesenchymal stem cells into cardiomyocytes induced by 5-azacytidine, Heart Journal 2004, 15(6)], angiotensin II [Shi Jingbao et al. Different concentrations of angiotensin Ⅱ induce the directed differentiation of bone marrow mesenchymal stem cells into cardiomyocytes, China Tissue Engineering Research Vol. Differentiation of Stem Cells into Cardiomyocytes, Chinese Tissue Engineering Research Volume 20, Issue 1] is a well-recognized inducer in the field that can induce bone marrow mesenchymal stem cells BMSCs to differentiate into cardiomyocytes. Therefore, in this example, these three substances were selected as inducers, and the differential m...

Embodiment 2

[0074] Example 2: Discovery of markers predicting the potential of bone marrow mesenchymal stem cells BMSCs to differentiate into nerve cells

[0075] 1. Experimental basis (inducers: salidroside, all-trans retinoic acid, methylcobalamin)

[0076] Salidroside [Zhang Quanwei et al., salidroside induced rat bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro, Chinese Tissue Engineering Research Volume 16 Issue 14], all-trans retinoic acid [Zhang Quanwei et al., Hong Sedroside induces rat bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro, Chinese Tissue Engineering Research Volume 16 No. 14], methylcobalamin [Yang Mingzhi et al., methylcobalamin induces rat bone marrow mesenchymal stem cells in vitro Differentiation of Stem Cells into Neuron-like Cells, Chinese Tissue Engineering Research Volume 17 Issue 32] is recognized in the field as an inducer that can induce bone marrow mesenchymal stem cells BMSCs to differe...

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Abstract

The invention discloses markers, a reagent and a reagent box for indicating the directional differentiation potential of mesenchymal stem cells (MSCs), in particular to markers, a reagent and a reagent box for indicating the potential that bone mesenchymal stem cells (BMSCs) directionally differentiate into cardiomyocyte-like cells. The makers are composed of alpha-oxoglutarate, dihydroxyacetone phosphate, asparagines and arachidonic acid. Results prove that the alpha-oxoglutarate, the dihydroxyacetone phosphate, the asparagines and the arachidonic acid are quite high in united indicating value, can be used as the markers for indicating the potential that the BMSCs differentiate into cardiomyocytes, and are high in efficiency when used for high throughput screening of inducers capable of inducing the BMSCs to differentiate into the cardiomyocytes, and people do not need to wait for several weeks to know an induction result.

Description

technical field [0001] The invention belongs to the field of stem cells, and relates to markers, reagents and kits for predicting the directed differentiation potential of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of multipotent adult stem cells, which exist in many kinds of tissues during human body generation and development. In the mid-1970s, Friedenstein et al [Friedenstein A J, Gorskaja J F, Kulagina N N. Fibroblast precursors in normaland irradiated mouse hematopoietic organs. Exp Hematol, 1976,4(5):267-274] obtained a small number of energy from bone marrow. Adherently growing cells, which can differentiate into osteogenic or chondrogenic deposits, are believed to be the first demonstration of the existence of MSCs. At present, MSCs have been isolated from various mesenchymal tissues of adults, and these cells can be expanded in vitro and differentiated into various connective tissue cells such as bone, cartilage, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02C12N5/077
CPCC12N5/0657C12N2506/1353G01N33/5044G01N33/5073
Inventor 高云雷其他发明人请求不公开姓名
Owner 南京千年健干细胞基因工程有限公司
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