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Method for producing oligopeptide for feed from intestinal residues

A technology of oligopeptides and intestinal residues, which is applied in animal feed, animal feed, applications, etc., can solve the problems of no pretreatment of intestinal residues, increase energy consumption, and affect enzymatic hydrolysis efficiency, so as to increase product competitiveness and reduce Product cost, the effect of improving product quality

Inactive Publication Date: 2017-03-29
大连平岛天然产物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 1. Intestinal residues used in research are dried and crushed intestinal residues powder instead of wet intestinal residues from the production line, which increases the energy consumption of drying and increases the production cost;
[0011] 2. There is no pretreatment of intestinal residue, which affects the enzymatic hydrolysis efficiency;
[0012] 3. No enzymes for enzymatic hydrolysis of intestinal residues were found for systematic screening;
[0013] 4. Enzymolysis with single enzyme only, no compound enzyme enzymolysis;
[0014] 5. The yield of enzymatic hydrolysis is low, only 67.34% (for protein)
[0015] 6. The molecular weight of the enzymatic hydrolysis product has not been determined, so the digestion and absorption rate of the product cannot be evaluated

Method used

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  • Method for producing oligopeptide for feed from intestinal residues

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0037] Specific implementation mode one: please refer to figure 1 , the specific embodiment adopts the following technical scheme: a kind of intestine dregs is the method for raw material production oligopeptide for feed, and its preparation method is:

[0038] a. Take 50g of intestinal residue, add 400ml of water, and stir to disperse;

[0039] b. Put the ingredients into the wall breaking machine and beat them into slurry;

[0040] c. Put the slurry into a beaker and heat it in a water bath to 40°C±1°C;

[0041] d. Use 10% NaOH seasoning solution pH7.5±0.1;

[0042] e. Add 0.263g of compound protease (2.5% total protein content), 0.08g of L-cysteine ​​hydrochloride (0.08%, w / v), and stir for 3.5h;

[0043] f. The pH of the feed liquid is adjusted to 5.5;

[0044] g. Heat up to 70°C and inactivate the enzyme for 20 minutes;

[0045] h. The feed liquid was centrifuged at 4000rpm for 10min;

[0046] i. The clear liquid is concentrated to a solid content of about 35% by a ...

specific Embodiment approach 2

[0049] Specific embodiment two: the preparation method of this specific embodiment is:

[0050] 1. Get 10kg of intestinal residue, add 100kg of water, stir and disperse;

[0051] II. Grinding the ingredients into a slurry through a colloidal mill;

[0052] III. Pour the slurry into an enzymatic hydrolysis tank and heat to 45°C±1°C;

[0053] IV. The feed liquid is adjusted to pH 8.0±0.1 with 10% NaOH;

[0054] V. Add 63.12g of compound protease (3.0% total protein), 55.0g of L-cysteine ​​hydrochloride (0.05%, w / v), and stir for 4 hours;

[0055] VI. The pH of the feed liquid is adjusted to 6.0;

[0056] VII. Heat up to 75°C and inactivate the enzyme for 15 minutes;

[0057] VIII, the feed liquid is filtered through the plate frame to get clear;

[0058] IX, the clear liquid is concentrated under reduced pressure to about 40% solid content;

[0059] X. Spray-dry the concentrate to obtain feed-grade oligopeptides.

specific Embodiment approach 3

[0060] Specific embodiment three: the preparation method of this specific embodiment is:

[0061] A. Take 100kg of intestinal residue, add 1500L of water, stir and disperse;

[0062] B. Grinding the ingredients into a slurry through a colloidal mill;

[0063] C. Pour the slurry into an enzymatic hydrolysis tank and heat to 50°C±1°C;

[0064] D. The feed liquid is adjusted to pH 8.5±0.1 with 10% NaOH;

[0065] E. Add 1.052 kg of compound protease (5.0% total protein), 1.6 g of L-cysteine ​​hydrochloride (0.1%, w / v), and stir for 5 hours;

[0066] F. The pH of the feed liquid is adjusted to 6.5;

[0067] G. Raise the temperature of the feed liquid to 80°C, and inactivate the enzyme for 10 minutes;

[0068] H. The feed liquid is filtered through the plate frame to get clear;

[0069] 1, the clear liquid is concentrated under reduced pressure to about 50% solid content;

[0070] J. Spray-dry the concentrate to obtain feed-grade oligopeptides.

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Abstract

The method discloses a method for producing oligopeptide for feed from intestinal residues and relates to the technical field of feed production. The method comprises adding a proper amount of water into intestinal residues, carrying out grinding through a colloid mill to obtain slurry, carrying out enzymolysis on the slurry through a compound protease and an enzymolysis accelerator, and carrying out filtration, concentration and spray drying to obtain the oligopeptide for feed. The method has simple processes, low investment in equipment, a high yield (greater than 90%), excellent product quality (oligopeptide content / total protein content of greater than or equal to 90%) and no three waste discharge and is suitable for promotion and use in small intestine deep processing enterprises.

Description

technical field [0001] The invention relates to the technical field of feed production, in particular to a method for producing oligopeptides for feed using intestinal residues as raw materials. Background technique [0002] Pig small intestine contains many useful substances, common ones are casing, plum blossom sausage, sodium heparin, dermatan sulfate, sulfatmonoesterase, Guanxinshu, etc. Hongyun et al., 2012), the solid matter remaining after extracting the above active ingredients is called intestinal residue. The content of protein in intestinal dregs varies with the process of extracting the substance, generally 20%-40% (Jiang Yan, 2001). [0003] There are many pig small intestine processing enterprises in my country, mainly processing casings and extracting crude heparin sodium. The annual production of intestinal residues is nearly one million tons. This is a very good protein resource for feed. If it can be comprehensively utilized, its resources can be fully uti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A23K20/147A23K10/14
CPCC12P21/06
Inventor 黄磊付佳奇刘心雨宋志凤
Owner 大连平岛天然产物科技有限公司
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