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Serum-free medium for in-vitro culture of epidermal melanocytes

A technology of serum-free medium and melanocytes, applied in the field of serum-free medium, can solve the problem of lack of stability of serum batches

Inactive Publication Date: 2017-03-22
严志海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to provide a new formulation of serum-free medium for in vitro culture of epidermal melanocytes, which solves the problems that prions may be contained in the serum and the batches of serum lack stability, etc.

Method used

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  • Serum-free medium for in-vitro culture of epidermal melanocytes
  • Serum-free medium for in-vitro culture of epidermal melanocytes

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Effect test

Embodiment 1

[0021] Embodiment 1 A kind of serum-free medium for culturing epidermal melanocytes in vitro

[0022] A serum-free medium for culturing epidermal melanocytes in vitro consists of the following components and their concentrations: human transferrin 15mg / L, insulin 10mg / L, glutamine 1mg / L, β-fibroblast growth factor 0.1ug / L, epidermal growth factor 5ug / L, epinephrine 50ug / L, streptomycin 1mg / L, calcium chloride 10mg / L, ferric nitrate 0.1mg / L, sodium selenite 1ug / L, copper sulfate 1ug / L, zinc sulfate 0.1mg / L, vitamin E 0.1mg / L, ethanolamine 0.1mg / L, phosphoethanol 0.1mg / L and dibutyryl cyclic adenosine monophosphate 6.2ug / L, the solvent is Ham's F12 basal medium.

Embodiment 2

[0023] Embodiment 2 A kind of serum-free medium for in vitro culture of epidermal melanocytes

[0024] A serum-free medium for in vitro culture of epidermal melanocytes consists of the following components and their concentrations: human transferrin 100mg / L, insulin 30mg / L, glutamine 10mg / L, β-fibroblast growth factor 5ug / L, epidermal growth factor 10ug / L, epinephrine 300ug / L, streptomycin 30mg / L, calcium chloride 50mg / L, iron nitrate 1mg / L, sodium selenite 10ug / L, copper sulfate 10ug / L , zinc sulfate 1mg / L, vitamin E 30mg / L, ethanolamine 50mg / L, phosphoethanol 0.1mg / L and dibutyryl cyclic adenosine monophosphate 5.3ug / L, the solvent is Ham's F12 basal medium.

Embodiment 3

[0025] Embodiment 3 A kind of serum-free medium for in vitro culture of epidermal melanocytes

[0026] A serum-free medium for culturing epidermal melanocytes in vitro consists of the following components and their concentrations: human transferrin 25mg / L, insulin 18mg / L, β-fibroblast growth factor 1ug / L, epidermal growth factor 6.5ug / L, epinephrine 75ug / L, streptomycin 8mg / L, calcium chloride 15mg / L, ferric nitrate 0.8mg / L, sodium selenite 4ug / L, copper sulfate 1.5ug / L, zinc sulfate 0.6 mg / L, vitamin E 9mg / L, ethanolamine 3mg / L, phosphoethanol 0.1mg / L and bisbutyryl cyclic adenosine monophosphate 5.4ug / L, the solvent is Ham's F12 basal medium.

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Abstract

The invention belongs to the field of cell culture media and, particularly relates to a serum-free medium for in-vitro culture of epidermal melanocytes. The serum-free medium is prepared from a basic medium, human transferrin, insulin, glutamine, Beta-fibroblast growth factor, epidermal growth factor, epinephrine, streptomycin, calcium chloride, iron nitrate, sodium selenite, copper sulfate, zinc sulfate, vitamin E, ethanolamine, ethanol phosphate and dibutyacyladenosine cyclophosphate. The ability of the serum-free medium to culture and breed epidermal melanocytes is equivalent to that of prior melanocyte media; the problems are solved that serum in serum media may bring prion and the batch of serum is instable; meanwhile, the serum-free medium can promote melanocytes to secrete melanin.

Description

technical field [0001] The invention belongs to the field of cell culture medium, in particular to a serum-free culture medium for culturing epidermal melanocytes in vitro. Background technique [0002] Skin color is primarily determined by the combination of pigments manufactured by the skin and natural pigments contained in the top layers of the skin. The epidermis on the surface of the epithelial layer of the skin contains epidermal melanocytes that produce skin pigment (melanin). In people with hypopigmented or amelanotic skin conditions, the melanocytes in the skin are missing, damaged, or nonfunctional, resulting in white spots on various parts of the body. [0003] Vitiligo is a special skin pigmentation disease characterized by white spots and loss of epidermal melanocytes. Current treatments include photosensitizers (eg, psoralen) combined with UVA irradiation, narrow-band UVB irradiation, or topical corticosteroids (adrenal), but have low efficacy and some side e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0626C12N2500/12C12N2500/14C12N2500/20C12N2500/22C12N2500/24C12N2500/25C12N2500/32C12N2500/38C12N2500/40C12N2500/42C12N2500/46C12N2500/90C12N2501/11C12N2501/115C12N2501/81
Inventor 严志海
Owner 严志海
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