Sterilization method of euglenophyta culture solution

Abstract

The invention relates to the technical field of euglenophyta culture, and discloses a sterilization method of an euglenophyta culture solution. The sterilization method of the euglenophyta culture solution comprises the steps of (1) taking an algae solution at a logarithmic growth phase, and centrifuging to obtain a supernatant; (2) resuspending algae cells through acidic heterotrophism or euglenophyta culture mediums, centrifuging, removing the supernatant, and then resuspending the algae cells through the acidic heterotrophism culture medium or the euglenophyta culture medium; (3) adding an antibiotic into a culture solution; (4) light culturing; and (5) selecting an inoculated algae solution survived after being processed through the step (4), and inoculating into a fresh sterile heterotrophism culture medium or a fresh sterile euglenophyta culture medium for culturing. According to the sterilization method of the euglenophyta culture solution provided by the invention, on the basis of adjusting a low pH value for a common culture medium, the antibiotic is added, so that the growth of infectious microbes can be remarkably inhibited, the growth of euglenophyta is promoted, the euglenophyta can quickly enter an exponential growth phase, higher growth density is achieved within a short time, and the dominant position of a population of the euglenophyta is maintained.

Description

technical field [0001] The invention relates to the technical field of Euglena culturing, in particular to a method for sterilizing a Euglena culture liquid. Background technique [0002] Euglena has quite high biological activity and has a good market prospect. However, the current level of industrial development is still relatively low, mainly because the existing Euglena culturing technology relies on high-cost and complex medium and culture methods, and the culture medium is rich in various nutrients, which makes it very easy to grow in The large-scale culture is contaminated by miscellaneous bacteria, which can lead to the failure of the entire batch of culture in severe cases, resulting in higher production costs and longer periods, which severely limits the scale and yield of large-scale culture. [0003] Under normal circumstances, the cultivation methods of Euglena are autotrophic and heterotrophic. The growth rate of autotrophic culture is slow, which is not cond...

AI Technical Summary

Problems solved by technology

The growth rate of autotrophic culture is slow, which is not conducive to the improvement of biomass and the accumulation of biologically active substances; currently, heterotrophic culture mostly uses artificial medium rich in nutrients such as proteoglycans, which contains organic substances such as yeast extract, beef extract, and peptone. Although the growth rate of euglena in this medium is fast, the price of raw materials is high, and it is very easy to be polluted by microorganisms such as bacteria, algae and protozoa in the open or large-scale cultivation process, which greatly reduces the production of euglena. Time may lead to the failure of the entire batch of large-scale cultivation, making it difficult to realize industrial production

[0004] The currently reported algae liquid sterilization methods mainly include: picking a single algal cell under a microscope, washing it repeatedly with a sterile algae medium, and then inoculating it in the medium. This method is complicated to operate and has high technical requirements for operators. , requires certain experience in algae classification, and at the same time, it is difficult to cultivate a single algal cell, and it is not easy to survive; use chemical substances to kill bacteria, including detergents such as sodium dodecylsulfonate (SDS), but chemical substances will also Cause damage to algae cells, different algae have different tolerances to detergents, some algae cells are very sensitive to this, euglena is quite sensitive to various detergents; using antibiotics to specifically kill bacteria, this It is the simplest and widely used method. The disadvantage is that different antibiotics have different effects on bacteria. Some antibiotics cannot completely kill bacteria, and some antibiotics can also cause damage to algae cells at the same time.

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