Method for preparing flower substrate from agricultural wastes
A technology for agricultural waste and flowers, applied in botany equipment and methods, planting substrates, preparation of organic fertilizers, etc., can solve the problems of long decomposing cycle, low nutrient content of fermented products, and difficulty in meeting the long-term growth of flowers, so as to avoid Pollution, increasing economic benefits, and reducing production costs
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Embodiment 1
[0026] (1) Preparation of corn flour extract: pulverize the dried corn with a pulverizer and pass it through a 20-mesh sieve. The obtained corn flour and phosphate buffer are mixed in a mass ratio of 1:2, and stirred at room temperature for 180 Minutes, let stand for 2 days, collect solid-phase corn flour precipitation and corn flour extract for use;
[0027] (2) Preparation of inoculum: using Saccharomyces cerevisiae ( Saccharomyces ), Aspergillus niger ( Aspergillus niger ), Trichoderma viride ( Trichoderma viride ), Acinetobacter ( Acinetobacter sp), Bacillus subtilis ( Bacillus subtilis ), Bacillus thuringiensis ( Bacillus thuringiensisBerliner ) Is the inoculation of microorganisms. The fungus is cultivated in potato dextrose liquid medium, and the bacteria is cultivated in beef extract peptone liquid medium, and 1% of the inoculum is connected to the corn meal extract. The total number of viable bacteria reaches 20 billion / ml. The number of bacteria is 1 billion / ml, ...
Embodiment 2
[0032] (1) Preparation of corn flour extract: pulverize the dried corn with a pulverizer and pass through a 20-mesh sieve, and mix the corn flour and phosphate buffer at a mass ratio of 1:1.5, and stir at room temperature for 150 Let stand for 2 days, collect solid corn flour and corn flour extract for use;
[0033] (2) Preparation of inoculum: using Saccharomyces cerevisiae ( Saccharomyces ), Aspergillus niger ( Aspergillus niger ), Trichoderma viride ( Trichoderma viride ), Acinetobacter ( Acinetobacter sp), Bacillus subtilis ( Bacillus subtilis ), Bacillus thuringiensis ( Bacillus thuringiensisBerliner ) Is the inoculation of microorganisms. The fungus is cultivated in potato dextrose liquid medium, and the bacteria is cultivated in beef extract peptone liquid medium, and 0.5% of the inoculum amount is connected to the corn flour extract. The total number of viable bacteria reaches 20 billion / ml. The number of bacteria is 1 billion / ml, Aspergillus niger is 2 billion / ml,...
Embodiment 3
[0038] (1) Preparation of corn flour extract: pulverize the dried corn with a pulverizer and pass through a 20-mesh sieve. The corn flour and phosphate buffer obtained are mixed at a mass ratio of 1:1, and stirred at room temperature for 120 Let stand for 2 days, collect solid corn flour and corn flour extract for use;
[0039] (2) Preparation of inoculum: using Saccharomyces cerevisiae ( Saccharomyces ), Aspergillus niger ( Aspergillus niger ), Trichoderma viride ( Trichoderma viride ), Acinetobacter ( Acinetobacter sp), Bacillus subtilis ( Bacillus subtilis ), Bacillus thuringiensis ( Bacillus thuringiensisBerliner ) Is the inoculation of microorganisms, cultured with beef extract peptone liquid medium, inoculated with corn meal extract at 0.6% inoculum, the total number of viable bacteria reaches 20 billion / ml, of which the number of live bacteria of Saccharomyces cerevisiae is 1 billion / ml and Aspergillus niger 1 billion / ml, Trichoderma viride 5 billion / ml, Acinetobact...
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